A5043963486- Cancer Research and Treatments
- Medical Imaging Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- Glycosylation and Glycoproteins Research
- Microbial Natural Products and Biosynthesis
- Meteorological Phenomena and Simulations
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Galectins and Cancer Biology
Shanghai Medical College of Fudan University
2023-2025
Natural products play a crucial role in new drug development, but their druggability is often limited by uncertain molecular targets and insufficient research on mechanisms of action. In this study, we developed RPL19-TRAPKI-seq method, combining CRISPR/Cas9 TRAP technologies, to investigate these mechanisms. We identified validated seven ribosomal large subunit surface proteins suitable for TRAP, selecting RPL19 its high enrichment. successfully established stable cell line expressing...
Abstract Aberrant N-linked glycosylation is a prominent feature of cancers. Perturbance oligosaccharide structure on cell surfaces directly affects key processes in tumor development and progression. In spite the critical role played by glycans biology, discovery small molecules that specifically disturbs still under investigation. To identify more saccharide-structure-perturbing compounds, repurposed drug screen using library consisting 1530 FDA-approved drugs was performed. Interestingly,...
<p>(a) Western blot analysis of protein expression pattern PD-L1 in HEK293T cells after the exposure to indicated concentrations penfluridol for 24 hours. (b) Protein B16-F10 treatment with 12 hours as determined by western blot. (c) The toxicity effect on (top) and B16-OVA (bottom) cells. (d) MAN1A1 treated siRNA.</p>
<p>(a) The stability of proteins was determined by cellular thermal shift at different concentrations penfluridol.</p>
<p>Supplementary Figure S1-S4 Supplementary Table S1</p>
<p>Supplementary Figure S1-S4 Supplementary Table S1</p>
<p>(a) Western blot analysis of glycoprotein patterns in MDA-MB-231 cells after treatment with the indicated concentrations penfluridol for 24 hours. Red arrowhead, immature glycoproteins. (b) protein expression pattern ACE2 HEK293T (c) In cells, FLAG-tagged was treated hours, collected by immunoprecipitation, subjected to SDS-PAGE, and analyzed western blot. (d) Pearson correlation coefficient three independent groups through N-glycan profiling.</p>
<p>(a) The stability of proteins was determined by cellular thermal shift at different concentrations penfluridol.</p>
<div>Abstract<p>Aberrant N-linked glycosylation is a prominent feature of cancers. Perturbance oligosaccharide structure on cell surfaces directly affects key processes in tumor development and progression. In spite the critical role played by glycans biology, discovery small molecules that specifically disturbs still under investigation. To identify more saccharide-structure-perturbing compounds, repurposed drug screen using library consisting 1530 FDA-approved drugs was...
<div>Abstract<p>Aberrant N-linked glycosylation is a prominent feature of cancers. Perturbance oligosaccharide structure on cell surfaces directly affects key processes in tumor development and progression. In spite the critical role played by glycans biology, discovery small molecules that specifically disturbs still under investigation. To identify more saccharide-structure-perturbing compounds, repurposed drug screen using library consisting 1530 FDA-approved drugs was...
<p>(a) Western blot analysis of protein expression pattern PD-L1 in HEK293T cells after the exposure to indicated concentrations penfluridol for 24 hours. (b) Protein B16-F10 treatment with 12 hours as determined by western blot. (c) The toxicity effect on (top) and B16-OVA (bottom) cells. (d) MAN1A1 treated siRNA.</p>
<p>(a) qPCR analysis of the GRP78 and CHOP expression in MDA-MB-231 cells after treatment with indicated concentrations for 6 hours. (b) Western blot Integrin β1 pattern drugs 24 (c) protein treated siRNA.</p>
<p>(a) Western blot analysis of glycoprotein patterns in MDA-MB-231 cells after treatment with the indicated concentrations penfluridol for 24 hours. Red arrowhead, immature glycoproteins. (b) protein expression pattern ACE2 HEK293T (c) In cells, FLAG-tagged was treated hours, collected by immunoprecipitation, subjected to SDS-PAGE, and analyzed western blot. (d) Pearson correlation coefficient three independent groups through N-glycan profiling.</p>
<p>(a) qPCR analysis of the GRP78 and CHOP expression in MDA-MB-231 cells after treatment with indicated concentrations for 6 hours. (b) Western blot Integrin β1 pattern drugs 24 (c) protein treated siRNA.</p>