A5041913075- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- Ferroptosis and cancer prognosis
- Bladder and Urothelial Cancer Treatments
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Medical Imaging Techniques and Applications
- Circadian rhythm and melatonin
- RNA Research and Splicing
- Trace Elements in Health
- Neonatal and fetal brain pathology
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Hepatocellular Carcinoma Treatment and Prognosis
- Immune cells in cancer
- Lung Cancer Diagnosis and Treatment
- Iron Metabolism and Disorders
- Prostate Cancer Treatment and Research
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- PI3K/AKT/mTOR signaling in cancer
- Heavy Metal Exposure and Toxicity
- Urinary and Genital Oncology Studies
Shanghai Medical College of Fudan University
2017-2025
Eye & ENT Hospital of Fudan University
2025
Fudan University
2012-2024
University of California, San Francisco
2015-2024
Zhongshan Hospital
2017-2024
BGI Research
2024
BGI Group (China)
2024
Nanjing Medical University
2023
Taizhou People's Hospital
2023
Nantong University
2023
Ischemic stroke (IS) is a detrimental neurological disease with limited treatments options. It has been challenging to define the roles of brain cell subsets in IS onset and progression due cellular heterogeneity CNS. Here, we employed single-cell RNA sequencing (scRNA-seq) comprehensively map populations mouse model MCAO (middle cerebral artery occlusion). We identified 17 principal clusters cell-type specific gene expression patterns as well subpopulations their functions various pathways....
Abstract Despite the potential adverse effects of maternal sleep deprivation (MSD) on physiological and behavioral aspects offspring, mechanisms remain poorly understood. The present study was intended to investigate roles microglia neurodevelopment cognition in young offspring rats with prenatal deprivation. Pregnant Wistar received 72 h last trimester gestation their prepuberty male were given intraperitoneal injection or without minocycline. results showed number Iba1 + increased, that...
Abstract Prostate cancer (PCa) is the second most prevalent malignancy in males across world. A greater knowledge of relationship between protein abundance and drug responses would benefit precision treatment for PCa. Herein, we establish 35 Chinese PCa primary cell models to capture specific characteristics among patients, including gene mutations, mRNA/protein/surface distributions, pharmaceutical responses. The multi-omics analyses identify Anterior Gradient 2 (AGR2) as a pre-operative...
Aberrations in the Hedgehog (Hh) signaling pathway are significantly prevailed various cancers, including B-cell lymphoma. A critical facet of Hh signal transduction involves dynamic regulation suppressor fused homolog (SUFU)-glioma-associated oncogene (GLI) complex within kinesin family member 7 (KIF7)-supported ciliary tip compartment. However, specific post-translational modifications SUFU-GLI this context have remained largely unexplored. Our study reveals a novel regulatory mechanism...
Members of the metallothionein (MT) family are short, cysteine-rich proteins involved in metal metabolism and detoxification, suggesting that MT protect cells from damage caused by electrophilic carcinogens thereby constitute a critical surveillance system against carcinogenesis. However, roles human hepatocellular carcinoma (HCC) not fully understood. We identified member family, termed MT1M. MT1M is expressed various normal tissues with highest level liver. expression can be induced heavy...
How multicellular organisms respond to and are impacted by severe hypothermic stress is largely unknown. From C. elegans screens for mutants abnormally responding cold-warming stimuli, we identify a molecular genetic pathway comprising ISY-1, conserved uncharacterized protein, ZIP-10, bZIP-type transcription factor. ISY-1 gatekeeps the ZIP-10 transcriptional program regulating microRNA mir-60. Downstream of mir-60, zip-10 levels rapidly specifically increase upon transient exposure....
Abstract Background State‐of‐art non‐invasive diagnosis processes for bladder cancer (BLCA) harbour shortcomings such as low sensitivity and specificity, unable to distinguish between high‐ (HG) low‐grade (LG) tumours, well inability differentiate muscle‐invasive (MIBC) non‐muscle‐invasive (NMIBC). This study investigates a comprehensive characterization of the entire DNA methylation (DNAm) landscape BLCA determine relevant biomarkers BLCA. Methods A total 304 samples from 224 donors were...
Abstract Background Psoriasis patients have relatively infrequent cutaneous viral infections compared to atopic dermatitis patients. Increased expression of four antiviral proteins ( MX 1, BST 2, ISG 15 and OAS 2) has been reported in psoriatic skin genetic studies psoriasis identified susceptibility genes pathways. Objective To determine if is associated with pervasive blood. Methods We performed RNA sequencing on samples 18 subjects chronic plaque 16 healthy controls. examined the a...
Abstract Inflammatory breast cancer (IBC) is the most aggressive form of cancer. Although it a rare subtype, IBC responsible for roughly 10% deaths. In order to obtain better understanding genomic landscape and intratumor heterogeneity (ITH) in IBC, we conducted whole-exome sequencing 16 tissue samples (12 tumor four normal samples) from six hormone-receptor-positive patients, analyzed somatic mutations copy number aberrations, inferred subclonal structures demonstrate ITH. Our results...
Natural products play a crucial role in new drug development, but their druggability is often limited by uncertain molecular targets and insufficient research on mechanisms of action. In this study, we developed RPL19-TRAPKI-seq method, combining CRISPR/Cas9 TRAP technologies, to investigate these mechanisms. We identified validated seven ribosomal large subunit surface proteins suitable for TRAP, selecting RPL19 its high enrichment. successfully established stable cell line expressing...
To examine the efficacy and safety of dupilumab treatment in moderate-to-severe atopic dermatitis (AD) patients with comorbid allergic rhinitis (AR). A total 26 received subcutaneous every 2 weeks were followed up for 16 weeks. The assessment was evaluated based on clinical parameters 4 weeks, including Eczema Area Severity Index (EASI), Pruritus Numeric Rating Scale (P-NRS), Dermatology Life Quality (DLQI) severity AD symptoms, nasal symptom scores (TNSS), visual analogue scale (VAS) Mini...
IL-2 is an important cytokine produced in T cells response to Ag or mitogen stimulation. It regulated at both transcriptional and posttranscriptional levels. One of the key regulators mRNA stability NF90. Upon cell activation, NF90 translocates from nucleus into cytoplasm, where it binds AU-rich element-containing 3' untranslated regions stabilizes it. Our previous work showed that CD28 costimulation activated AKT phosphorylate Ser(647) caused undergo nuclear export stabilize mRNA. Phorbol...
We collected clinical data from 308 prostate cancer (PCa) patients to investigate the characteristics and independent risk factors of bone metastasis (BM) establish a prediction model for BM PCa determine necessity scans.Univariate multivariate analyses were performed based on age, biopsy Gleason score (BGS), tumor stage (cTx), total specific antigen (tPSA), free (fPSA), fPSA/tPSA, volume, alkaline phosphatase (ALP), serum calcium phosphorus.Moreover, 80 had PI-RADS v2 analysed...
Controlling translation initiation is an efficient way to regulate gene expression at the post-transcriptional level. However, current knowledge regarding regulatory proteins and their modes of controlling still limited. In this study, we employed tandem affinity purification mass spectrometry screen for unknown associated with machinery. Ubiquitin specific peptidase 9, X-linked (USP9X), was identified as a novel binding partner, that interacts eukaryotic factor 4B (eIF4B) in...
Abstract: Non-small cell lung cancer (NSCLC) patients with anaplastic lymphoma kinase ( ALK ) rearrangement benefit from treatment inhibitors. Therefore, the identification of druggable fusions is necessary for NSCLC treatment. More than 90 fusion partners have been reported in patients, but striatin gene STRN -ALK has rarely reported. Moreover, response - treated inhibitors remains to be explored. A 64-year-old Chinese male no history smoking or alcohol consumption was diagnosed as stage...
Induction of hypothermia during hibernation/torpor enables certain mammals to survive under extreme environmental conditions. However, pharmacological induction in most remains a huge challenge. Here we show that natural product P57 promptly induces and decreases energy expenditure mice. Mechanistically, inhibits the kinase activity pyridoxal (PDXK), key metabolic enzyme vitamin B6 catalyzing phosphorylation (PL), resulting accumulation PL hypothalamus cause hypothermia. The induced by is...
The Drosophila homolog of cAMP-response element-binding protein (CREB), dCREB2, exists with serine 231, equivalent to mammalian 133, in a predominantly phosphorylated state. Thus, unlike the protein, primary regulation dCREB2 may occur at different step from 231 phosphorylation. Although bacterially expressed bound element sites, extracts was unable do so unless treated phosphatase. Phosphorylation recombinant by casein kinase (CK) I or II, but not calcium-calmodulin II A, inhibited DNA...
Abstract Background The 8th edition of the American Joint Committee on Cancer (AJCC) staging system for gallbladder carcinoma (GBC) came into force since 2018. However, prognostic precision this has not been properly assessed. This study aimed to evaluate latest and suggest modifications improve its precision. Methods Data patients with GBC was included from Surveillance, Epidemiology End Results (SEER) database (2004–2015) multicenter (2010–2017). Baseline clinicopathologic characteristics...
Chinese Giant Salamanders (Andrias davidianus) have experienced an 80% population decline since the 1950s. Captive salamander farms in China might provide a source for reintroduction projects involving this species. To recommendations on rearing individuals reintroduction, we studied effect of water temperature growth captive-reared juvenile (2–6 yr) A. davidianus and compared their body condition with wild specimens. We reared 240 salamanders two groups, exposed them to different...
Wilson disease (WD) is a rare autosomal recessive genetic disorder that associated with various mutations in the ATP7B gene. Although variants are frequently identified, exact mutation patterns remain unknown because of absence pedigree studies, and functional consequences individual to be clarified. In this study, we recruited 65 clinically diagnosed WD patients from 60 unrelated families. Pedigree analysis showed besides several homozygous (8/65, 12.3%), compound heterozygous (43/65,...