A5036760119- Probiotics and Fermented Foods
- Gut microbiota and health
- Digestive system and related health
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Advanced Topology and Set Theory
- Pediatric health and respiratory diseases
- Infant Nutrition and Health
- Microscopic Colitis
- Microbial Metabolites in Food Biotechnology
- Bacterial Identification and Susceptibility Testing
- Diphtheria, Corynebacterium, and Tetanus
- Nutrition, Genetics, and Disease
- Microbial Community Ecology and Physiology
- Oral microbiology and periodontitis research
- Urinary Tract Infections Management
- Reproductive tract infections research
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- Advanced Banach Space Theory
- Cancer Research and Treatments
- Mathematical and Theoretical Analysis
- Human Health and Disease
- Mathematical Dynamics and Fractals
- Effects of Radiation Exposure
Federal Medical-Biological Agency
2023-2025
Pirogov Russian National Research Medical University
2015-2025
Ministry of Health of the Russian Federation
2008-2024
Federal Agency for Health and Social Development
2008
Lomonosov Moscow State University
2008
Central Economics and Mathematics Institute
1974-1994
Gorno-Altaisk State University
1979
Moscow State Pedagogical University
1979
Geological Institute
1967
Introduction Bacteroides fragilis (Bf)’s T6SS locus has been characterized and shown to have functional activity in competition experiments. It demonstrated that symbiont nontoxigenic Bf strains a more effective “weapon” effect on pathogenic Bf, which is realized through the of effector-immune (E-I) protein pairs. Intensive study structure led an understanding certain issues related its activity, but exact regulatory mechanisms E-I pair remain unclear. Proteomic annotation components...
Certain Bifidobacterium strains have been shown to inhibit inflammatory responses in intestinal epithelial cells. However, the precise mechanisms of these effects, including chemical nature active compounds, remain be elucidated. Here partial characterization anti-inflammatory properties isolated from feces healthy infants is reported. It was found that conditioned media (CM) all studied are capable attenuating tumor necrosis factor-α (TNF-α) and lipopolysaccharide- (LPS) induced HT-29 cell...
Culture-based study of the faecal microbiome in two adult female subjects revealed presence obligately anaerobic, non-spore-forming, rod-shaped, non-motile, Gram-negative bacterial strains that represent novel species. The first strain, designated 627 T , was a fastidious, slow-growing, indole-positive bacterium with non-fermentative type metabolism. strain characterized by production acetic and succinic acids as metabolic end products, prevalence iso-C 15 : 0 fatty acid menaquinones MK-10...
Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces healthy human subjects. The strains, designated as 585-1T and 668, characterized by mesophilic fermentative metabolism, production d-lactic acid, succinic acid acetic end products d-glucose fermentation, prevalence C18 : 1ω9, 1ω9 aldehyde, C16 0 1ω7c fatty acids, presence glycine, glutamic lysine, alanine aspartic in petidoglycan peptide moiety lack...
A novel obligately anaerobic, non-spore-forming, rod-shaped, non-motile Gram-reaction-negative bacterium was isolated from infant faeces. The strain, designated NSB1(T), able to grow on rich media at 30-37 °C, in the presence of up 2 % (w/v) Oxgall and NaCl. Cells strain NSB1(T) produced catalase, but not urease indole. Aesculin hydrolysed. utilize d-glucose, lactose, maltose, mannose raffinose as electron donors. When grown main metabolic end products were propionic acetic acids, with a...
Members of genus Bifidobacterium are Gram-positive bacteria, representing a large part the human infant microbiota and moderately common in adults. However, our knowledge about their diversity, intraspecific phylogeny long-term persistence humans is still limited. longum generally considered to be most prevalent species intestinal microbiota. In this work we studied whole genome sequences 28 strains B. longum, including 8 described paper. Part these were isolated from healthy children during...
Microorganisms and their hosts communicate with each other by secreting numerous components. This cross-kingdom cell-to-cell signaling involves proteins small molecules, such as metabolites. These compounds can be secreted across the membrane via transporters may also packaged in outer vesicles (OMVs). Among components, volatile (VOCs) are of particular interest, including butyrate propionate, which have proven effects on intestinal, immune, stem cells. Besides short fatty acids, groups...
A study of species distribution numerically predominant Bacteroidales order isolates in feces healthy people aged 1-33 years was accomplished using a combination amplified ribosomal DNA restriction analysis (ARDRA) and 16S (rDNA) sequencing. It found that the majority all age groups belonged to B. xylanisolvens, vulgatus, uniformis. Members genera Alistipes, Parabacteroides, Odoribacter, Barnesiella, Prevotella were also detected frequently.
ABSTRACT Inflammatory bowel diseases are characterized by chronic intestinal inflammation and alterations in the gut microbiota composition. Bacteroides fragilis , which secretes outer membrane vesicles (OMVs) with polysaccharide A (PSA), can moderate inflammatory response possibly alter In this study, we created a murine model of sodium dextran sulfate (DSS)-induced colitis treated it B. OMVs. We monitored efficiency OMV therapy determining disease activity index (DAI) performing...
Inflammatory bowel disease (IBD) is highly prevalent globally and, in the majority of cases, remains asymptomatic during its initial stages. The gastrointestinal microbiota secretes volatile organic compounds (VOCs), and their composition alters IBD. examination VOCs could prove beneficial complementing diagnostic techniques to facilitate early identification IBD risk. In this protocol, a model sodium dextran sulfate (DSS)-induced colitis rats was successfully implemented for non-invasive...
Three novel strains of Gram-stain-negative, obligately anaerobic, spore-forming straight or slightly curved rods with pointed ends occurring singly in pairs were isolated from the faeces healthy human children. The characterized by mesophilic fermentative metabolism and production acetate, ethanol H2 as end metabolic products. Strains ASD3451 ASD5720T motile, fermented lactose raffinose, weakly maltose. Strain ASD4241T was non-motile did not ferment carbohydrates listed above but starch....
A strain of obligately anaerobic, Gram-stain-negative rods was isolated from human faeces and characterized both phenotypically genotypically. Phylogenetic analysis based on 16S rRNA gene whole-genome sequences revealed the to represent a member genus Prevotella, distant species with validly published names, closest relationship Prevotella oryzae. The moderately saccharolytic proteolytic. predominant menaquinones were MK-13 MK-12. major cellular long-chain fatty acids anteiso-C15 : 0 iso-C15...
Bifidobacteria and Bacteroides-like bacteria are strictly anaerobic nonpathogenic members of human intestinal microflora. Here we describe an analysis the species subspecies composition these bacterial populations in healthy children using a combination culture molecular methods at two different time points. It was found that B. bifidum longum most common dominant taxons infants aged between 8 16 months. The majority carried several Bifidobacterium strains belonging to species. Examination...
The members of genus Bifidobacterium represent a significant part intestinal microbiota in adults and predominate infants. Species repertoire the bifidobacteria is known to be subjected major changes with age; however, many details this process are still elucidated.Our aim was study diversity their qualitative quantitative composition characteristics during growing up using MALDI-TOF mass-spectrometric analysis ofpure bacterial cultures.A cross-sectional performed 93 healthy people ages from...
A study of the faecal microbiome in three healthy female rhesus macaques revealed presence a novel obligately anaerobic, chemoorganoheterotrophic, non-sporing, coccoid, non-motile, Gram-stain-positive bacterial species. Three strains this species, designated as M108T, M916-1/1, and M919-2/1, were non-haemolytic, H2S-positive, catalase-positive, bile- NaCl-sensitive required peptone for growth. Strains also asaccharolytic, able to utilize sulfite, thiosulfate elemental sulfur electron...
Bifidobacteria constitute a significant part of healthy intestinal microbiota in adults and infants present promising platform for construction genetically modified probiotic agents treatment gastrointestinal disorders. In this study, three strains Bifidobacterium longum were constructed that express secrete biologically active single-chain antibodies against human TNF-α Clostridium difficile exotoxin A. Anti-TNF-α scFv antibody D2E7 was produced at the level 25 μg L(-1) broth culture mostly...
We prove that a dyadic space of weight contains the Cantor cube if and only it cannot be represented as countable union closed subsets with weights less than . A similar result has been independently obtained by Gerlits. That solves problem posed Pelczynski. In particular case when is, in addition, Dugundji space, recently solved Haydon. Further, follows any whose is not sum countably many smaller cardinals can continuously mapped onto Tihonov This true, particular, regular cardinal, was...
A strain of obligately anaerobic, spore-forming, Gram-positive rods was isolated from child faeces and characterized both phenotypically genotypically. Phylogenetic analysis based on 16S rRNA gene whole genome sequencing revealed the to represent a member family Ruminococcaceae distant described species genera. The moderately saccharolytic with mannose as preferred substrate produced lactic acid, acetic acid H2 end products. major cellular long-chain fatty acids were C16 : 0 aldehyde....