A5081891279- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- Biofuel production and bioconversion
- Antioxidant Activity and Oxidative Stress
- Microplastics and Plastic Pollution
- Metabolomics and Mass Spectrometry Studies
- Biocrusts and Microbial Ecology
- Aquatic Ecosystems and Phytoplankton Dynamics
- CRISPR and Genetic Engineering
- Marine and coastal ecosystems
- Enzyme Production and Characterization
- Plant biochemistry and biosynthesis
- Microbial Metabolites in Food Biotechnology
- Plant Gene Expression Analysis
- biodegradable polymer synthesis and properties
- Catalysis for Biomass Conversion
- Lignin and Wood Chemistry
- Cancer, Hypoxia, and Metabolism
- Fermentation and Sensory Analysis
- Spaceflight effects on biology
- Phytase and its Applications
- Chemistry and Chemical Engineering
- Biomedical and Engineering Education
Washington University in St. Louis
2021-2025
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2018-2022
Tianjin University
2014-2022
Ministry of Education of the People's Republic of China
2018-2022
Xuzhou University of Technology
2010
More than 70 million tons of poly(ethylene terephthalate) (PET) are manufactured worldwide every year. The accumulation PET waste has become a global pollution concern, motivating the urgent development technologies to valorize post-consumer PET. chemocatalytic and enzymatic approaches for depolymerizing its corresponding monomers opens up new opportunities upcycling through biological transformation. Here, we identify Rhodococcus jostii strain (RPET) that can directly use hydrolysate as...
Docosahexaenoic acid (DHA, C22:6) and odd-chain fatty acids (OCFAs, C15:0 C17:0) have attracted great interest, since they been widely used in food therapeutic industries, as well chemical industry, such biodiesel production improvement. The oil-producing heterotrophic microalgae Schizochytrium sp. 31 is one of main DHA-producing strains. Recently, it was found that can also synthesize OCFAs; however, contents titers DHA OCFAs are still low, which limit its practical application. In this...
Photosynthetic cyanobacteria have attracted a significant attention as promising chassis to produce renewable fuels and chemicals due their capability utilizing solar energy CO2. Notably, the enhancing supply of key precursors like malonyl-CoA would benefit production many bio-compounds. Nevertheless, lacking genetic tools in cyanobacteria, especially knockdown strategies for essential pathways, has seriously restricted attempts re-direct carbon flux from central carbohydrate metabolism...
Cyanobacterial carbohydrates, such as sucrose, have been considered potential renewable feedstock to support the production of fuels and chemicals. However, separation purification processes these carbohydrates will increase cost Co-culture fermentation has proposed an efficient economical way utilize cyanobacterial carbohydrates. studies on application co-culture systems achieve green biosynthesis platform chemicals are still rare.In this study, we successfully achieved one-step conversion...
In-space biomanufacturing provides a sustainable solution to facilitate long-term, self-sufficient human habitation in extraterrestrial environments. However, its dependence on Earth-supplied feedstocks renders in-space economically nonviable. Here, we develop process termed alternative feedstock-driven in-situ (AF-ISM) alleviate Earth-based resupply of feedstocks. Specifically, investigate three (AF)—Martian and Lunar regolith, post-consumer polyethylene terephthalate, fecal waste—to an...
In cyanobacteria, photomixotrophic growth is considered as a promising strategy to achieve both high cell density and product accumulation. However, the conversion of glucose acetyl coenzyme A (acetyl-CoA) in native glycolytic pathway insufficient, which decreases carbon utilization productivity engineered cyanobacteria under conditions. To increase flux from key intracellular precursor acetyl-CoA Synechocystis sp. PCC 6803 (hereafter, 6803) conditions, synthetic nonoxidative cyclic...
In this study, we evaluated suitable selected markers and optimized transformation protocols to develop a new genetic methodology for DHA-producing C. cohnii. Additionally, ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), potentially involved in CO2 fixation under autotrophic conditions, was as the target construction of gene knockdown mutant. Our results show that constructs were successfully inserted into cohnii chromosome by homologous recombination. Comparative analysis showed...
The heterotrophic microalga Crypthecodinium cohnii has attracted considerable attention due to its capability of accumulating lipids with a high fraction docosahexaenoic acid (DHA). In our previous study, ethanolamine (ETA) was identified as an effective chemical modulator for lipid accumulation in C. cohnii. this gain better understanding the metabolism and mechanism positive effects ETA, metabolic flux analysis performed using 13C-labeled glucose without 1 mM ETA modulator. distribution...
Rhodococcus opacus is a nonmodel bacterium that well suited for valorizing lignin. Despite recent advances in our systems-level understanding of its versatile metabolism, studies gene functions at single level are still lagging. Elucidating organisms challenging due to limited genetic engineering tools convenient use. To address this issue, we developed simple repression system based on CRISPR interference (CRISPRi). This uses T7 RNA polymerase express small guide RNA, demonstrating improved...
Docosahexaenoic acid (DHA, 22:6n-3) plays significant roles in enhancing human health and preventing diseases. The heterotrophic marine dinoflagellate
Abstract Background Docosahexaenoic acid (DHA) is essential for human diet. However, high production cost of DHA using C . cohnii makes it currently less competitive commercially, which mainly caused by low productivity. In recent years, repeated fed-batch strategies have been evaluated increasing the many fermentation products. The reduction in terms stability culture system was one major challenges fermentation. possible mechanisms responsible decreased are so far investigated, restricting...
Abstract Rhodococcus opacus PD630 has considerable potential as a platform for valorizing lignin due to its innate “biological funneling” pathways. However, the transcriptional regulation of aromatic catabolic pathways and mechanisms controlling operons in response different mixtures are still underexplored. Here, we identified studied transcription factors degradation using GFP-based sensors comprehensive deletion analyses. Our results demonstrate that funneling phenol, guaiacol,...
Lycium barbarum contains high levels of zeaxanthin, which is produced by the conversion -carotene into zeaxanthin. -Carotene hydroxylase catalyzes this reaction. We cloned a cDNA (chyb) encoding (Chyb) from L. leaf. A 939-bp full-length sequence was determined with 3′-rapid amplification end assay deduced Chyb protein (34.8 kDa) theoretical isoelectric point 8.36. bioinformatics analysis showed that located in chloroplast. Further, to investigate catalytic activity Chyb, complementation...