This study aimed to probe into the associations among circular RNA ZFR (circ-ZFR), miR-130a/miR-107, and PTEN, investigate regulatory mechanism of circ-ZFR‒miR-130a/miR-107‒PTEN axis in gastric cancer (GC).

The aim of this study was to investigate the effects exosomes isolated from human bone marrow mesenchymal stem cells (BMSCs) on osteoarthritis (OA) and a competitive endogenous RNA (ceRNA) network.Exosomes were BMSCs characterized by transmission electron microscopy (TEM), Nanosight (NTA), western blotting. Chondrocytes treated with interleukin-1β (IL-1β) then transfected exosomes. Cell viability apoptosis determined using Counting Kit-8 (CCK-8) flow cytometry, respectively. Cells IL-1β...

Glycogen is a highly branched glucose polymer which involved in maintaining blood-sugar homeostasis. Liver glycogen contains large composite α particles made up of linked β particles. Previous studies have shown that the binding links into impaired diabetic mice. The present study reports first molecular structural characterization human-liver from non-diabetic patients, using transmission electron microscopy for morphology and size-exclusion chromatography size distribution; latter also...

Paclitaxel is an effective chemotherapeutic agent for the treatment of cancer patients. Accumulating evidence suggests that circular RNAs (circRNAs) play critical roles in occurrence and development human cancers. However, there are few studies on interactions between paclitaxel circRNAs hepatocellular carcinoma (HCC).Cell counting kit-8 (CCK-8) assay colony formation were conducted to determine cell proliferation. Cell apoptosis was assessed by flow cytometry. The expression levels circRNA...

Early embryonic lung branching morphogenesis is regulated by many growth factor-mediated pathways. Bone morphogenetic protein 4 (BMP4) one of the morphogens that stimulate epithelial in mouse explant culture. To further understand molecular mechanisms BMP4-regulated development, we studied biological role Smad-ubiquitin regulatory factor 1 (Smurf1), an ubiquitin ligase specific for BMP receptor-regulated Smads, during development. The temporo-spatial expression pattern Smurf1 was first...

Background Transcatheter closure is now the preferred treatment for congenital heart disease complicated with pulmonary arterial hypertension (CHD-PAH), but its long-term effects are not well understood. We aimed to assess safety, effectiveness, and outcome of this procedure in children CHD-PAH. Methods included 210 CHD-PAH at our hospital from 2012 2021 collected their general, laboratory, echocardiographic, hemodynamic data analysis. A logistic regression analysis identified risk factors...

The majority of stage III non-small cell lung cancer (NSCLC) is unresectable. However, effective treatment may enable patients to transition resectable NSCLC, thereby extending the progression-free survival time. current study presents case a female patient with IIIC unresectable cancer. received icotinib followed by neoadjuvant aumolertinib, resulting in significant tumor shrinkage and downstaging meet resectability criteria. Despite ~16 months targeted therapy, including resistance both...

The expression of progestin and adipoQ receptor 3 (PAQR3) is generally downregulated in multiple tumors, which associated with tumor progression poor prognosis. clinical value PAQR3 was analyzed using various databases 60 patients non-small cell lung cancer (NSCLC). In addition, counting kit-8 (CCK-8), colony formation, flow cytometry assays were used to evaluate the effect on growth NSCLC cells vitro. Gene set enrichment analysis (GSEA) performed investigate possible mechanism through...

Lipopolysaccharide (LPS) is regarded as the main pathogenic factor of periodontitis. Mesenchymal stem cell-derived small extracellular vesicles (sEVs) play a key role in variety physiological and pathological processes. This study investigated effects sEVs derived from periodontal ligament cells (PDLSCs) pretreated with LPS on macrophage polarization underlying mechanisms. PDLSCs were treated (1 µg/mL) for 24 h, harvested by gradient centrifugation method. Macrophages incubated followed...

Retinoic acid induced 14 (RAI14), also known as NORPEG, is reported being deregulated in non-small-cell lung cancer, together with having involvement its cell proliferation a super enhancer related gene.The objective of this study was to investigate the role RAI14 progression and metastasis gastric cancer explore associated mechanism.GEPIA database used analyze expression cancer. MNK45 AGS cells were transfected siRNA-RAI14 block RAI14. Cell Counting Kit 8 colony formation assays performed...

Tuberculosis (TB), is an infectious disease caused by Mycobacterium tuberculosis ( M. tuberculosis), and presents with high morbidity mortality. Alveolar macrophages play important role in TB pathogenesis although there heterogeneity functional plasticity. This study aimed to show the characteristics of alveolar from bronchioalveolar lavage fluid (BALF) active patients. Single-cell RNA sequencing (scRNA-seq) was performed on BALF cells three patients additional scRNA-seq data healthy adults...

Eukaryotic translation initiation factor 5A2 (EIF5A2) has been demonstrated to be upregulated in numerous types of human cancer and is associated with progression. However, the expression role EIF5A2 non-small cell lung (NSCLC) remains unclear. In present study, NSCLC was investigated, addition underlying molecular mechanisms by which acts. Relative levels were determined cells compared non-cancerous tissues. Short interfering (si)RNA targeted against used knock down cells. Cell...

Abstract Background Increasing evidence has indicated that circular RNAs (circRNAs) play a role in various diseases. However, the influence of circRNAs nephritis remains unknown. Methods Microarray analysis and RT-qPCR were used to detect expression circRNA. Type I IFN administrated RMC HEK293 cells establish cell model. CCK-8, MTT assay, flow cytometry assess proliferation, viability, apoptosis cells. Bioinformatics dual luciferase reporter assay interaction circ_0007059, miRNA-1278, SHP-1....

Background: Recent studies showed that sushi repeat containing protein X linked 2 (SRPX2) could participate in the development of various malignant tumors. However, its role non-small cell lung cancer (NSCLC) was unknown. The aim study to prospectively investigate SRPX2 NSCLC proliferation, migration and invasion reveal underlying mechanism. Material methods: Quantitative real-time polymerase chain reaction (qRT-PCR), western blot immunohistochemistry – IHC) were used measure detect mRNA...

Bone morphogenetic protein (BMP) 4 plays very important roles in regulating developmental processes of many organs, including lung. Smad1 is one the BMP receptor downstream signaling proteins that transduce BMP4 ligand from cell surface to nucleus. The dynamic expression patterns embryonic mouse lungs were examined using immunohistochemistry. was predominantly detected peripheral airway epithelial cells early lung tissue [embryonic day 12.5 (E12.5)], whereas mesenchymal increased during...

We previously reported a strategy for expression and purification of human Vasostatin120-180 (VAS), potent angiogenesis inhibitor in GST fusion form; however, the yield 7.2 mg per liter culture was relatively low. The aim this study to develop more efficient system improve facilitate production VAS protein soluble native form Escherichia coli. gene with optimized condons cloned into pET28a overexpressed as N-terminal His-tagged protein. Between His-tag VAS, an enterokinase recognition site...

Esophageal squamous cell carcinoma (ESCC) is a kind of malignant tumor with high incidence and mortality in the digestive system. The aim this study to explore function lnc-ABCA12-3 development ESCC its unique mechanisms. RT-PCR was applied detect gene transcription levels tissues or lines like TE-1, EC9706, HEEC cells. Western blot conducted identify protein expression mitochondrial apoptosis toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) signaling pathway. CCK-8 EdU assays were...

Circular RNAs have emerged as important regulators in the pathogenesis of human diseases, including infantile pneumonia (IP). In this study, we aimed to explore effects circ_0035292 on lipopolysaccharide (LPS)-treated Wistsar Institute (WI)-38 cells.

Background: Circular RNAs (circRNAs) may play important roles in the progression of pulmonary arterial hypertension.However, potential they childhood hypertension associated congenital heart disease (CHD) remains unclear.Methods: Thirteen human plasma samples including eight from secondary to CHD patients and five a control group were analyzed using Arraystar Human circRNA array.The relative expression levels differentially expressed circRNAs detected real-time polymerase chain reaction...