Sepsis is a systemic inflammatory response syndrome caused by infection, with high incidence and mortality. Therefore, it necessary to carry out an effective anti-infection treatment. In this work, we designed synthesized red blood cell (RBC) membrane-coated PLGA nanoparticles named γ3-RBCNPs, which target the highly expressed intercellular adhesion molecule-1 (ICAM-1) at site of infection through γ3 peptide on its surface kill Klebsiella pneumoniae ciprofloxacin encapsulated in core....

Introduction: The emergence of multidrug-resistant Klebsiella pneumoniae ( K. ) and the decline effective antibiotics lead to urgent need for new antibacterial agents. aim this study is investigate therapeutic effect antimicrobial peptides against gentamicin-resistant (RT) screen peptides. Methods: In study, RT strains were induced by gradient gentamicin, selected detecting expression levels efflux pump genes, porin biofilm formation genes combined with their effects on cells. Then four...

The gut microbiome has been considered to play an important role in inflammatory bowel disease (IBD). Our previous study reported that tacrolimus-altered microbiota elicited immunoregulatory effects both colonic mucosa and circulation, contributing increased allograft survival rate mice. Here, we aimed observe the changes tacrolimus-induced a dextran sulfate sodium (DSS)-induced colitis mouse model explore possibility efficacy of combination therapy with tacrolimus on colitis. Mice were...

Abstract The effect of low‐FODMAPs diet on irritable bowel syndrome (IBS) in Western China has not been reported. We aimed to investigate the IBS patients area and whether diet‐induced alterations microbiota could be improved through probiotics. were randomized control group, probiotics or combined group. Symptom Severity Score questionnaire (IBS‐SSS) Quality Life (IBS‐QOL) completed at baseline, 2 4 weeks evaluate severity symptoms. Fresh feces collected for analyses gut short‐chain fatty...

is an opportunistic pathogen causing severe infections. The circadian rhythm the internal mechanism of organism and plays important role in coping with changes 24-h rhythm. Disruption can lead to immune, behavioral, mental, other related disorders. Whether

STING activation is a promising application therapeutic strategy for cancer immunotherapy. In particular, MSA-2 as an oral agonist discovered to have antitumor activity. However, how improve the effect of very valuable contribution Here, we use two strategies amplify by phospholipid nanoparticles delivering mRNA and copper-modified MSA-2. We synthesized new series ionizable optimized nanoparticle (1AP24) mRNA, increasing expression protein bind more Second, (MSA-2-Cu), which induced cell...

The type III secretion system (T3SS) of Edwardsiella piscicida plays a crucial role in its pathogenesis. Our previous study indicated that the T3SS effector protein EseJ inhibits bacterium’s adhesion to epithelioma papillosum cyprini (EPC) cells, while mechanism inhibition remains elusive.

The type III secretion system effector EseJ plays a regulatory role inside bacteria. It suppresses the adherence of Edwardsiella piscicida (E. piscicida) to host epithelial cells by down regulating 1 fimbriae. In this study, we observed that more macrophages infected with ΔeseJ strain E. detached as compared those wild-type (WT) strain. Terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining and cleaved caspase-3 examination revealed detachment is due increased...

In this study, a hypothetical protein (ORF02740) secreted by Edwardsiella piscicida was identified. We renamed the ORF02740 as EvpQ, which is encoded mobile genetic element (MGE) in E. genome. The evpQ gene spaced 513 genes from type VI secretion system (T6SS) cluster. Low GC content, three tRNA, and transposase nearby define MGE that localizes genomic island. Sequence analysis reveals EvpQ shares conserved domain of C70 family cysteine protease 23.91% identity with T3SS effector AvrRpt2...

Both bacteria and autophagy are implicated in inflammatory bowel disease (IBD) pathogenesis. However, how crosstalk with signaling remains largely known, especially intestinal mucosa. This study aimed to profile the internal complex cascade their external correlation these bacteria, consequently provide a systematic precise target for future IBD diagnosis therapy. We found Ulcerative colitis (UC) patients exhibited more severe dysbiosis than Crohn’s (CD) patients, as represented by alpha...

Abstract Aeromonas salmonicida is a gram‐negative bacterium that the causative agent of furunculosis. An A. strain was isolated from diseased turbot ( Scophthalmus maximus ) with sign furunculosis North China. Based on vapA gene, further classified as subsp. masoucida RZ6S‐1. Culturing RZ6S‐1 at high temperature (28°C) obtained virulence attenuated RZ6S. Genome sequence comparison between two strains revealed loss type IV secretion system (T4SS) and III (T3SS) native plasmid pAsmB‐1 pAsmC‐1...

Type III secretion system (T3SS) facilitates survival and replication of Edwardsiella piscicida in vivo. Identifying novel T3SS effectors elucidating their functions are critical understanding the pathogenesis E. piscicida. effector EseG EseJ was highly secreted when gatekeeper-containing protein complex EsaB-EsaL-EsaM disrupted by EsaB deficiency. Based on this observation, concentrated secretomes ΔesaB strain ΔesaBΔesaN were purified loading them into SDS-PAGE gel for a short...

The type III secretion system (T3SS) is one of the most important virulence factors fish pathogen Edwardsiella piscicida It contains three translocon proteins, EseB, EseC, and EseD, required for translocation effector proteins into host cells. We have previously shown that EseB forms filamentous appendages on surface E. piscicida, these structures mediate bacterial cell-cell interactions promoting autoaggregation biofilm formation. In present study, we show but not inhibits formation At 18 h...

By studying the expression in patients and cell modeling vitro, antimicrobial peptides for Klebsiella were screened. Killing curve membrane permeability experiments are used to study antibacterial effect of vitro. Cytotoxicity-related indicators including lipopolysaccharide (LPS), capsule polysaccharide (CPS), outer protein measured. Intranasal inoculation pneumoconiosis was construct a mouse infection model, survival rate cytokine level tested. Human neutrophil peptide 1 (HNP-1) showed...

Owing to the parasitic vibration effect of cooling medium and pipes X-ray optics, decoupling method based on eutectic gallium-indium (EGaIn) alloy has become very crucial for fourth generation synchrotron radiation advanced light sources. However, there is an issue that corrosion EGaIn heat sink metal [e.g., copper (Cu) plate] results in solidification failure eliminating effect. To deal with problem, a novel anti-corrosion coating tungsten (W) presented this paper. It possesses better...

Type 1 fimbria, the short hair-like appendage assembled on bacterial surface, plays a pivotal role in adhesion and invasion

Abstract Background MSA-2, as an oral molecule for activating STING signaling pathway to cure the tumor entering clinical trials. The toxicity of MSA-2 has aroused wide concern, especially reproductive can not be ignored. Objectives We synthesized agonist (MSA-2) and its derivative manganese-MSA-2 (MSA-2-Mn) investigated toxicity. Methods evaluated effects MSA-2-Mn in female mice under administration alone on system male presence or absence combined radiation. Results suggested that have...

The photoactive anode was fabricated by hydrothermal method using the ZnO nanorod array as template. NiO nanoflakes were assembled on TiO 2 tubular arrays to form p-n junction heterostrucutures Ni substrate. water electrolysis coupled with photocatalytic decomposition of irradiation UV and UV-visible light modified anode. Under irradiation, hydrogen evolution rates NiO/TiO nanotube composites are 2.92 ml/h·cm 3.16 respectively, increased 5.4 % 15 in comparison that showed 152 172 improvement...

The atom transfer radical polymerization is successful for synthesizing the topological type PMMA with α-D-glucose/2-bromoisobutyryl bromide as initiating system, CuBr catalyzer and PMDETA ligand. synthesized by such method has a controllable relative molecular mass structure. narrow distribution of really achieves activity ATRP.