A5100386899- Influenza Virus Research Studies
- Respiratory viral infections research
- Immune Response and Inflammation
- interferon and immune responses
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Advanced Drug Delivery Systems
- Tuberculosis Research and Epidemiology
- vaccines and immunoinformatics approaches
- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- Metabolomics and Mass Spectrometry Studies
- Monoclonal and Polyclonal Antibodies Research
- Plant-based Medicinal Research
- Traditional Chinese Medicine Analysis
- Hepatitis B Virus Studies
- Viral Infections and Outbreaks Research
- Acute Myeloid Leukemia Research
- Vector-borne infectious diseases
- RNA modifications and cancer
- Traditional Chinese Medicine Studies
Bengbu Medical College
2023-2025
Shenyang Medical College
2024-2025
Nanjing University of Chinese Medicine
2013-2024
Heilongjiang University of Chinese Medicine
2022
Hunan Normal University
2009-2021
Guangxi University
2020
Zhongnan Hospital of Wuhan University
2020
Wuhan University
2020
China Pharmaceutical University
2009-2018
Harvard University
2015
ABSTRACT Influenza is a highly infectious disease characterized by recurrent annual epidemics and unpredictable major worldwide pandemics. Rapid spread of the pathogenic avian H5N1 strain escalating human infections virus have set off alarm for global pandemic. To provide an urgently needed alternative treatment modality influenza, we generated recombinant fusion protein composed sialidase catalytic domain derived from Actinomyces viscosus fused with cell surface-anchoring sequence. The to...
Increasing resistance to currently available influenza antivirals highlights the need develop alternate approaches for prevention and/or treatment of influenza. DAS181 (Fludase), a novel sialidase fusion protein that enzymatically removes sialic acids on respiratory epithelium, exhibits potent antiviral activity against A and B viruses. Here, we use mouse model evaluate efficacy highly pathogenic avian H5N1 virus. When used treat mice daily beginning 1 day before infection with...
Background The recent emergence of a novel pandemic influenza A(H1N1) strain in humans exemplifies the rapid and unpredictable nature virus evolution need for effective therapeutics vaccines to control such outbreaks. However, resistance antivirals can be formidable problem as evidenced by currently widespread oseltamivir- adamantane-resistant seasonal A viruses (IFV). Additional antiviral approaches with mechanisms action are needed combat resistant strains. DAS181 (Fludase™) is sialidase...
Background. The first step in infection by human parainfluenza viruses (HPIVs) is binding to the surface of respiratory epithelial cells via interaction between viral receptor-binding molecules and sialic acid-containing receptors. DAS181, a recombinant sialidase protein containing catalytic domain Actinomyces viscosus sialidase, removes cell acid, we proposed that it would inhibit HPIV infection. Methods. Depletion acid receptors DAS181 was evaluated lectin-binding assays. Anti-HPIV...
Antiviral drug resistance for influenza therapies remains a concern due to the high prevalence of H1N1 2009 seasonal isolates which display H274Y associated oseltamivir-resistance. Furthermore, emergence novel raises potential that additional reassortments can occur, resulting in resistant virus. Thus, antiviral approaches are urgently needed. DAS181 (Fludase), sialidase fusion protein, has been shown have inhibitory activity against large number strains and highly pathogenic avian (HPAI)...
ABSTRACT DAS181 is a novel candidate therapeutic agent against influenza virus which functions via the mechanism of removing receptor, sialic acid (Sia), from adjacent glycan structures. and its analogues have previously been shown to be potently active multiple strains seasonal avian in several experimental models, including cell lines, mice, ferrets. Here we demonstrate that treatment leads desialylation both α2-6-linked α2-3-linked Sia ex vivo human lung tissue culture primary...
Parainfluenza virus (PIV) can cause significant morbidity after allogeneic stem cell transplantation (SCT). We report the first use of inhaled DAS181 for PIV in an SCT recipient. Symptoms, oxygenation, and pulmonary function tests improved. Nasopharyngeal samples showed a reduction viral load. should be systematically evaluated severe infection.
Abstract Parainfluenza virus ( PIV ) infections can cause serious respiratory and death in immunocompromised patients. No antiviral agents have proven efficacy against , therapy generally consists of supportive care. DAS 181, a novel sialidase fusion protein that temporarily disables airway epithelial receptors by enzymatic removal sialic acid moieties, has been shown to inhibit infection with strains vitro an animal model. We describe here the clinical course 2 patients ‐3 infection, one...
The influenza virus (IFV) infection models commonly used to evaluate antiviral agents (e.g. MDCK cell line and mice) are limited by physiological differences from the human respiratory tract in vivo. Here we report pharmacodynamics of DAS181, a sialidase fusion protein that inhibits infection, model systems well-defined airway epithelium (HAE) culture ex vivo fresh bronchial tissue, both which close mimics vivo.HAE bronchi were sialic acid removal regeneration efficiency IFV inhibition after...
The present study was designed to determine the effects of Ganoderma lucidum polysaccharides (GL-PS) on exhaustive exercise-induced oxidative stress in skeletal muscle tissues mice. mice were divided into four groups (three GL-PS administered and control group). group with distilled water (50, 100 200 mg/kg body weight per day). After 28 days, performed an swimming exercise, along determination superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) activities...
Keywords: self-double-emulsifying drug delivery system, hydroxysafflor yellow A, Caco-2 cells, bioavailability, histopathologic studies
We performed an annotation of 35 mutations in the spike protein SARS-CoV-2 Omicron variant. Our analysis indicates that has gained prominent immune evasion and potential for enhanced transmissibility. Previous modeling study revealed continued evolution both transmissibility by would compromise vaccines as tools pandemic control. To combat future variants SARS-CoV-2, world needs novel antiviral drugs are effective at curb viral spreading without introducing additional selective pressure...
Influenza viruses (IFVs) frequently achieve resistance to antiviral drugs, necessitating the development of compounds with novel mechanisms action. DAS181 (Fludase®), a sialidase fusion protein, may have reduced potential for generating drug due its host-targeting mechanism IFV strains B/Maryland/1/59 and A/Victoria/3/75 (H3N2) were subjected >30 passages under increasing selective pressure DAS181. The DAS181-selected isolates characterized in vitro mice. Despite extensive passaging,...
DAS181 (Fludase) is a sialidase fusion protein in clinical development as broad-spectrum anti-influenza virus (IFV) therapeutic agent. Previous reports by other investigators have raised the concern that desialylation of airway epithelium might increase susceptibility to Streptococcus pneumoniae infection.To address whether would lead an increased risk pneumococcal infection, we tested S. colonization after treatment human A549 cells, healthy mice, and mice challenged with lethal dose IFV...
We selected the conserved sequence in stalk region of influenza virus hemagglutinin (HA) trimmer, long alpha helix (LAH), as vaccine candidate sequence, and inserted it into major immunodominant (MIR) hepatitis B core protein (HBc), and, by using E. coli expression system, we prepared a recombinant LAH-HBc form virus-like particles (VLP). Intranasal immunization mice with this VLP plus cholera toxin subunit 0.2% (CTB * ) adjuvant could effectively elicit humoral cellular immune responses...
Ovarian cancer (OC) is the most deadly gynecological tumor. OC cells utilize cellular metabolic reprogramming to gain a survival advantage, particularly through aberrant lipid process. As primary ingredient in exogenous cannabinoids, cannabidiol (CBD) has been confirmed exhibit antitumor activity preclinical studies. However, it still unclear whether CBD can disrupt fatty acid metabolism and induce apoptosis cells. In this study, we have demonstrated that significantly inhibits proliferation...