A5100642294- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Catalysis for Biomass Conversion
- Advanced Algorithms and Applications
- Advanced Sensor and Control Systems
- Industrial Technology and Control Systems
- Circular RNAs in diseases
- Chaos control and synchronization
- Nanowire Synthesis and Applications
- Analog and Mixed-Signal Circuit Design
- Scheduling and Optimization Algorithms
- Vibration and Dynamic Analysis
- Optimization and Search Problems
- Semiconductor Quantum Structures and Devices
- Quantum Dots Synthesis And Properties
- Advanced Manufacturing and Logistics Optimization
- Advancements in Semiconductor Devices and Circuit Design
- Nonlinear Dynamics and Pattern Formation
- Chemical Synthesis and Analysis
- Statistical Mechanics and Entropy
- Complex Systems and Time Series Analysis
- Fuzzy Logic and Control Systems
- Fermentation and Sensory Analysis
- MicroRNA in disease regulation
Shandong Academy of Sciences
2018-2025
Qilu University of Technology
2016-2025
The Central Hospital of Shaoyang
2025
Second Xiangya Hospital of Central South University
2025
Central South University
2025
Central Hospital of Zibo
2024-2025
Lanzhou University
2025
First Hospital of Lanzhou University
2025
Gansu Provincial Center for Disease Control and Prevention
2025
Hohai University
2024
Abstract Despite the well‐recognized merits of simultaneous saccharification and co‐fermentation (SSCF) on relieving sugar product inhibition cellulase activity, a practical concomitance difficulty xylose with inhibitors in pretreated lignocellulose feedstock prohibits essential application SSCF for cellulosic ethanol fermentation. To maximize potentials production, dry biorefining approach was proposed starting from acid pretreatment, disk milling, biodetoxification feedstock. The...
The cost-effective production of second-generation bioethanol, which is made from lignocellulosic materials, has to face the following two problems: co-fermenting xylose with glucose and enhancing strain's tolerance inhibitors. Based on our previous study, wild-type diploid Saccharomyces cerevisiae strain BSIF robustness good metabolism genetic background was used as a chassis for constructing efficient xylose-fermenting industrial strains. performance resulting strains in fermentation media...
Prolyl thioesters have shown significantly lower reactivities in native chemical ligation (NCL) comparison to that of the alanyl thioester. This report describes a mild and efficient internal activation protocol peptidyl prolyl NCL without using any thiol-based additives, where introduction 4-mercaptan substituent on C-terminal proline improves reactivity via formation bicyclic thiolactone intermediate. The kinetic data indicate reaction rate is comparable reported thioesters, mechanistic...
N-Glycosylation represents an essential type of posttranslational modification for proteins. However, deciphering the functions N-glycosylation remains a challenge due to lack analytical and biochemical methods accurately differentiate protein glycoforms with various intact glycans. Here we report our synthesis evaluation homogeneously glycosylated interleukin-17A (IL-17A), based on synthetic approach combining solid-phase (glyco)peptides, chemoenzymatic glycan segments, chemical ligations....
Summary To develop a suitable S accharomyces cerevisiae industrial strain as chassis cell for ethanol production using lignocellulosic materials, 32 wild‐type strains were evaluated their glucose fermenting ability, tolerance to the stresses they might encounter in hydrolysate fermentation and genetic background pentose metabolism. The BSIF , isolated from tropical fruit T hailand, was selected out of distinctly different studied its promising characteristics. maximal specific growth rate...
The economical and efficient commercial production of second-generation bioethanol requires fermentation microorganisms capable entirely rapidly utilizing all sugars in lignocellulosic hydrolysates. In this study, we developed a recombinant
Conversion of various lignocellulosic materials into bioethanol is growing in demand but greatly depends on feedstock availability. Dairy cow manure an agricultural waste widely distributed worldwide. This study investigated the induction cellulases by and conversion ethanol. Alkaline NaOH pretreatment improved accessibility lignocellulose to enzymes followed enzymatic hydrolysis using Penicillium oxalicum cellulases. The ethanol yields from pretreated anaerobically digested were 0.19 0.13...
Abstract It has been suggested that circular RNAs play critical roles in natural growth and disease development. Nevertheless, whether the were related Hirschsprung's (HSCR) remains unknown. Thus, we discovered cir‐CCDC66 was downregulated HSCR compared with normal gut tissues. The reduction might inhibit cells’ proliferation migration vitro. Then, found DCX transcript putative competing endogenous RNA. Furthermore, function of as a sponge for miR‐488‐3p to regulate RNA expression...
Efficient utilization of both glucose and xylose from lignocellulosic biomass would be economically beneficial for biofuel production. Recombinant Saccharomyces cerevisiae strains with essential genes metabolic networks metabolism can ferment xylose; however, the efficiency fermentation is much lower than that glucose, preferred carbon source yeast. Implications our previous work suggest activation sensing system may benefit metabolism. Here, we show deleting cAMP phosphodiesterase PDE1 PDE2...
For bioethanol production from lignocellulosic materials, an ideal microorganism must possess both excellent xylose utilization and a high tolerance to inhibitory compounds. However, these two traits often exhibit antagonism in recombinant xylose-utilizing Saccharomyces cerevisiae strains. In this study, we developed quantitative metric using aggregated parameter evaluate the degree of applied it three strains (LF1, LF1-6M, 6M-15), which had been iteratively evolved hydrolyzate environments....
<title>Abstract</title> <bold>Background</bold> The genetic etiology of epilepsy is highly heterogeneous and complex. Copy number variation sequencing (CNV-seq) whole exome (WES) have emerged as effective tools for identifying causes in patients with unexplained epilepsy. This study aimed to investigate the etiology, evaluate diagnostic utility concurrent CNV-seq WES, provide evidence precision medicine counseling. <bold>Methods</bold> We conducted a retrospective cohort 283 undergoing WES...
Circular RNAs (circRNAs) are a novel class of noncoding (ncRNAs), which have been shown to participate in intracellular RNA regulatory networks and play vital roles many pathological processes. Recently, circular RNA_PRKCI (circ-PRKCI) has reported regulate cell proliferation, migration invasion several human cancers. Hirschsprung disease (HSCR) is well-known congenital gut motility disorder roots the aberrance cranial-caudal neural crest migration. In this study, we investigated whether...
The carrier recombination dynamics in ZnSe nanowires (NWs) remain poorly understood despite more than a decade of research since their inception 2001. Herein, through comprehensive pump fluence‐ and temperature‐dependent two‐photon excitation (TPE) study, clear picture the relaxation pathways, intrinsic lifetimes, exciton oscillator strengths, exciton‐phonon interactions is presented for this NW system. Contrary to common perception that higher intensities needed achieve two‐photon‐excited...
Summary Exorbitant outputs of waste xylose mother liquor (WXML) and corncob residue from commercial‐scale production xylitol create environmental problems. To reduce the wastes, a Saccharomyces cerevisiae strain tolerant to WXML was conferred with abilities express genes reductase, xylose‐specific transporter enzymes pentose phosphate pathway. This showed high capacity produce in glucose as co‐substrate. Additionally, simultaneous saccharification fermentation (SSF) process designed use...
Peptidyl thioesters or their surrogates with C-terminal β-branched hydrophobic amino acid residues usually exhibit poor reactivities in ligation reactions. Thus, activation using exogenous additives is required to ensure an acceptable reaction efficiency. Herein, we report a traceless at Val-Xaa sites under mild thiol additive-free conditions, whereby the introduction of β-mercaptan on valine residue effectively activates otherwise unreactive N-acyl-benzimidazolinone (Nbz), and enables use...
This brief presents a sub-microwatt oversampling successive approximation register (SAR) analog-to-digital converter (ADC) dedicated to Internet of Things sensing applications. Several techniques are adopted in this design enable ultra-low power consumption while achieving 15-bit accuracy. The ground sampling technique minimizes DAC switching energy without requiring common mode voltage comparator with dynamic bit trial settings achieves low noise efficient manner using two-stage...
Abstract During second‐generation bioethanol production from lignocellulosic biomass, the desired traits for fermenting microorganisms, such as Saccharomyces cerevisiae , are high xylose utilization and robustness to inhibitors in hydrolysates. However, observed previously, these two easily showed antagonism, one rising other falling, C6/C5 co‐fermenting S. strain. In this study, LF1 obtained our previous study is an engineered budding yeast strain with a superior co‐fermentation capacity of...