A5084755300- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Acute Myocardial Infarction Research
- Cardiac Imaging and Diagnostics
- Aquaculture disease management and microbiota
- Sepsis Diagnosis and Treatment
- Veterinary Equine Medical Research
- Hemodynamic Monitoring and Therapy
- Heart Failure Treatment and Management
- Parathyroid Disorders and Treatments
- Herpesvirus Infections and Treatments
- Venous Thromboembolism Diagnosis and Management
- Bone health and treatments
- Clinical Laboratory Practices and Quality Control
- Cardiac electrophysiology and arrhythmias
- Bacterial Genetics and Biotechnology
- Toxoplasma gondii Research Studies
- Hemoglobinopathies and Related Disorders
- Erythropoietin and Anemia Treatment
- Plant Virus Research Studies
- Healthcare cost, quality, practices
- Iron Metabolism and Disorders
- Cytomegalovirus and herpesvirus research
- Potassium and Related Disorders
- Blood groups and transfusion
Aarhus University
1991-2024
Aarhus University Hospital
2012-2024
University of Copenhagen
1990-2023
Laboratoire de Biochimie
2016
Swedish University of Agricultural Sciences
2015
Central Denmark Region
2013
Copenhagen University Hospital
1998-2011
Rigshospitalet
2002-2011
Capital Region of Denmark
2011
Hvidovre Hospital
1990-1998
We present the complete maps of five Mycoplasma hominis genomes, including a detailed restriction map and locations number genetic loci. The fragments were resolved by field inversion gel electrophoresis or contour-clamped homogeneous-electric-field system pulsed-field electrophoresis. All ApaI, SmaI, BamHI, XhoI, SalI sites (total 21 to 33 in each strain) placed on physical map, yielding an average resolution 26 kb. constructed using three different approaches: (i) size determination DNA...
Purpose: In patients with a suspected acute myocardial infarction (AMI), to evaluate the potential for early triage based on measurement of high-sensitivity cardiac troponin T (hs-cTnT) and copeptin in blood samples collected prehospital phase.Materials methods: this retrospective study, we measured hs-cTnT ambulance form 962 AMI. The diagnostic accuracy was estimated by receiver-operating characteristic (ROC) curve area under (AUC) both biomarkers combined model. Multivariable Cox...
BACKGROUND: Dialysis catheters are a common cause of nosocomial septicaemia in haemodialysis units usually due to staphylococci, which Staphylococcus aureus is the most pathogenic. In this study, epidemiology and pathogenesis dialysis catheter-related infections were studied, methods identify patients with these evaluated. METHODS: A one-year prospective study 67 43 was performed. Details about obtained successively during catheter period, biochemical parameters expected be related infection...
Abstract Background: Patients on chronic hemodialysis are prone to develop amyloid deposits of misfolded β2-microglobulin (β2M) in osteoarticular tissues. β2M with various deletions/truncations and chemical modifications has been found together structurally intact extracts fibrils. The state the circulating population molecules not characterized previously high-resolution methods. Methods: We used immunoaffinity–liquid chromatography–mass spectrometry analysis serum samples examine whether...
Familial hypocalciuric hypercalcemia (FHH) type 1 is caused by mutations in the gene encoding calcium-sensing receptor (CASR). Recently, affecting codon 15 AP2S1 have been shown to cause FHH 3 up 26% of CASR-negative patients. Similarly, GNA11 2. We hypothesized that and are causative Danish patients with suspected these not found primary hyperparathyroidism (PHPT), which main differential diagnostic disorder.Cross-sectional study.We identified unexplained hyperparathyroid a control group...
BackgroundPazopanib can induce liver toxicity in patients with metastatic renal cell carcinoma (mRCC). We assessed the effect of a TA repeat polymorphism UGT1A1 (uridine diphosphate glucuronosyltransferase 1A1) gene encoding uridine 1A1 on toxicity, dose reductions, and patient outcomes.Patients MethodsPatients mRCC treated first-line pazopanib developing underwent genotyping for polymorphism. Liver was using Common Terminology Criteria Adverse Events, version 4.0. Progression-free survival...
The in vivo distribution of the intercellular adhesion molecule ICAM-1 was investigated renal tissue specimens obtained from 17 allotransplanted patients, nine normal kidneys (controls), seven native kidneys, patients with mesangioproliferative glomerulonephritis, and active extracapillary glomerulonephritis. Biopsies signs acute rejection showed a significant increase expression tubular epithelium (P< 0.05). In (controls) found endothelial cells; additional epithelial cells induced vitro...
Background Cardiac troponins are the preferred biomarkers for diagnosis of acute myocardial infarction. Although sex‐specific 99th percentile thresholds recommended in international guidelines, clinical effect their use is poorly investigated. The DANSPOT Study (The Danish Sex‐ and Population‐Specific upper reference limits Troponin) aims to evaluate a prospective implementation population‐ diagnostic into practice. Methods This study nationwide, multicenter, stepped‐wedge cluster‐randomized...
To determine the relative prognostic merits of C-reactive protein (CRP), cardiac troponin T (cTnT) and N-terminal pro-brain natriuretic peptide (NT-pro-BNP) for prediction all-cause death in patients with end-stage renal disease (ESRD) receiving haemodialysis.This prospective, controlled cohort study included 109 patients. Biomarkers were sampled at inclusion considered as categorical continuous variables Cox proportional hazard models.Mean follow-up ± SD was 926 385 days, during which 52...
M. hominis is commonly found as part of the normal flora in female genital tract, but several studies have shown that it may be involved a variety urogenital infections. The basis for clinical manifestations some patients has varyingly been attributed to host and factors. factors infection process are largely unknown. no cell wall outer membranes, at present seems plausible possesses genetic systems allowing bacteria vivo alter its antigenic structure on membrane surface consequently...
The homolog of the gyrB gene, which has been reported to be present in vicinity initiation site replication bacteria, was mapped on Mycoplasma hominis genome, and region subsequently sequenced. Five open reading frames were identified flanking one showed similarity that encodes LicA protein Haemophilus influenzae. organization genes no resemblance corresponding regions other bacteria sequenced so far. gyrA gene 35 kb downstream from gene.
Mycoplasma hominis contains a variable adherence‐associated ( vaa ) gene. To classify variants of the genes, we examined 42 M. isolates by PCR, DNA sequencing and immunoblotting. This uncovered existence five gene categories. Comparison types revealed modular composition Vaa proteins. The proteins constituted conserved N‐terminal part followed varying number interchangeable cassettes encoding approximately 110 amino acids with sequence boxes flanking cassettes. showed high mutual homology...
A monoclonal antibody was used to characterize a 135-kDa surface-located membrane protein (Lmp1) generally present in Mycoplasma hominis strains. The antibody, 552, applied identify the corresponding gene an expression library of M. PG21 DNA. lmp1 sequenced, and its product characterized with goal elucidating structure function Lmp1. total 7,196 bp region sequenced. An open reading frame 4,032 bp, encoding 1,344 amino acids calculated molecular weight 147,000, identified. Analysis deduced...
The effect of dressings saturated with either a standardized suspension probiotic bacteria or saline on healing traumatic distal limb wounds in horses was evaluated for 24 days, and the systemic inflammatory assessed. were divided two groups based phase healing: an incomplete (ICGB) complete granulation bed (CGB). wound area expressed as percentage at day 0 defined relative area. mean decreased faster than treated wounds. difference most obvious CGB increased rapidly from until 12 up to 30%,...
Three mutants of Mycoplasma hominis PG21 were isolated and shown to contain alterations in the size a repeat-containing gene encoding surface-localized 135-kDa antigen designated Lmp1. The by cultivating M. for 3-month period presence Lmp1-specific monoclonal antibody (MAb) 552. epitope MAb 552 was localized at repeated part protein. Lmp1 is transcriptional complex that contains 9.5 direct repeats 471 bp each. Pure cultures mutant strains obtained subcloning, three characterized. showed...
Mycoplasma hominis, an opportunistic pathogenic bacterium of humans, has a small genome 700 kb. Despite this, multiple copies gene sequences with similarities to the structural (lmp1) 135-kDa surface-located membrane protein (Lmp1) have been identified on M. hominis PG21 (lmp2, lmp3, and lmp4). The distance between lmp1-lmp2 region lmp3-lmp4 was more than 110 sequenced found contain two putative genes. 6.5 kb contained 5' unique 3' separated by 9 0.5-kb repeats 51 90% similarity 10 similar...
During haemodialysis (HD) ultrafiltration may affect the central blood volume to an extent that pressure decreases. Thoracic electrical impedance (TI) is applied monitor and we evaluated if it can be used predict HD-induced hypotension.In 12 hypotensive prone (H) 13 non-hypotensive (N) patients, heart rate were recorded during one dialysis session every 30 min, while TI, thoracic intracellular water (Th(ICW)) total body (TBI) followed 10 min. Hypotension was defined as a decrease in systolic...
Background: The serum-angiotensin I converting enzyme (s-ACE) activity is influenced by a genetic insertion/deletion (I/D) polymorphism in the ACE gene, and resulting large interindividual variation s-ACE limits use of normal reference intervals evaluation sarcoidosis. In this study, we developed new method for genotyping I/D established genotype-specific order to improve diagnostic accuracy value treatment Methods: assay based on high-resolution melting (HRM) using LCGreen + was used...