A5037747202- Advanced biosensing and bioanalysis techniques
- Distributed systems and fault tolerance
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- MicroRNA in disease regulation
- Energetic Materials and Combustion
- DNA and Nucleic Acid Chemistry
- Traffic Prediction and Management Techniques
- Software System Performance and Reliability
- Thermal and Kinetic Analysis
- Service-Oriented Architecture and Web Services
- Plant Stress Responses and Tolerance
- Transportation Planning and Optimization
- Molecular Junctions and Nanostructures
- Evaluation Methods in Various Fields
- Safety and Risk Management
- Nanopore and Nanochannel Transport Studies
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Traffic control and management
- Advanced Data Storage Technologies
- Thermochemical Biomass Conversion Processes
Chinese Academy of Sciences
2018-2025
Suzhou Institute of Biomedical Engineering and Technology
2018-2025
University of Science and Technology of China
2018-2025
Research Center for Eco-Environmental Sciences
2025
University of Chinese Academy of Sciences
2025
Heilongjiang Provincial Academy of Agricultural Sciences
2019-2025
Suzhou University of Science and Technology
2025
Foshan University
2019-2024
Gansu Provincial Hospital
2023
Didi Chuxing (China)
2020-2023
Nucleic acids, including circulating tumor DNA (ctDNA), microRNA, and virus DNA/RNA, have been widely applied as potential disease biomarkers for early clinical diagnosis. In this study, we present a concept of nanostructures transitions the construction bipedal walking nanomachine, which integrates dual signal amplification direct nucleic acid assay. hairpins transition is developed to facilitate generation multiple target sequences; meanwhile, subsequent dumbbell-wheel controlled achieve...
In yeast, Sir2 family proteins (sirtuins) regulate gene silencing, recombination, DNA repair, and aging via histone deacetylation. Most of the seven mammalian sirtuins (Sirt1–Sirt7) have been implicated as NAD + -dependent protein deacetylases with targets ranging from transcriptional regulators to metabolic enzymes. We report that neural-specific deletion sirtuin 6 (Sirt6) in mice leads postnatal growth retardation due somatotropic attenuation through low hormone (GH) insulin-like factor 1...
Classical nonhomologous DNA end-joining (C-NHEJ), which is a major double-strand break (DSB) repair pathway in mammalian cells, plays dominant role joining DSBs during Ig heavy chain (IgH) class switch recombination (CSR) activated B lymphocytes. However, cells deficient for one or more requisite C-NHEJ factors, such as ligase 4 (Lig4) XRCC4, CSR occurs by distinct alternative (A-EJ) pathway. A-EJ also has been implicated found oncogenic chromosomal translocations. 3 (Lig3) and its cofactor...
DNAs are one of the most fundamental molecules for life. Quantification specific sequences is great importance biological research and clinical diagnosis. In order to determine extremely low abundant DNAs, we herein develop a novel electrochemical genosensor taking advantage smart bipedal DNA walking machine. Magnetic nanomaterials first employed enrich target DNA. Strand displacement amplification initiated by then designed on surface nanomaterials, products which can be used trigger an...
A three-dimensional DNA tetrahedral nanostructure is constructed to support a walker strand on top and multiple track strands around it via the assembly of triplex-forming oligonucleotide (TFO). This design facilitates regeneration sensing interface by simply adjusting pH conditions. On basis supported walking nanomachine, ultrasensitive electrochemical analysis miRNA (miR-141) achieved. Target assists formation three-way junction nanostructure. It contains duplex region (hybridized strands)...
Circulating tumor DNA (ctDNA) serves as a powerful noninvasive and viable biomarker for the diagnosis of cancers. The abundance ctDNA in patients with advanced stages is significantly higher than that early stages. Herein, ratiometric electrochemical biosensor detection developed by smart design probes recycles DNAzyme activation. conformational variation structures leads to changes two types species. This enzyme-free sensing strategy promotes excellent amplification efficiency upon target...
Manganese dioxide (MnO2) nanosheets are emerging for biomedical applications with excellent physical and chemical properties. Adsorption of DNA on MnO2 is important biosensing, bioimaging, therapy. Nevertheless, current fundamental understanding about the interaction preliminary. Herein, UV-vis absorption spectra applied to systematically explore biointerfacial between factors salt concentration, pH value, temperature, length. The results offer insights into investigation DNA-MnO2...
China is currently at a crucial stage of systematically advancing the construction sponge cities and completely implementing "synergetic enhancement pollution carbon emission reduction." The reduction benefits during operational phase source control facilities, as well synergistic path must be crucially understood scientifically. Taking 30 city projects in Wuxi, Jiangsu Province examples, we analyzed benefits, costs, determined relationship among them phase. Additionally, proposed three by...
Abstract 3D hybrids of interconnected porous carbon (PC) nanosheets/vertically aligned polyaniline (Pani) nanowires are prepared by a cost‐effective facile approach and used as high‐performance supercapacitor electrodes. The PC/Pani obtained simple in situ growth Pani on PC with high specific surface area good electrochemical performance fabricated via one‐step thermal pyrolysis potassium citrate. show an excellent capacitance 512 F g −1 at 1 A the three‐electrode configuration, which is...
Low temperature is one of the main environmental factors that limits crop growth, development, and production. Medicago falcata an important leguminous herb widely distributed worldwide. M. related to alfalfa but more tolerant low than alfalfa. Understanding tolerance mechanism for genetic improvement alfalfa.In this study, we explored transcriptomic changes in roots low-temperature-treated plants by combining SMRT sequencing NGS technologies. A total 115,153 nonredundant sequences were...
Herein, we have developed an ultrasensitive self-powered biosensor for miRNA assay based on biofuel cells. The system is composed of indium tin oxide cathode and graphene oxide/gold nanoparticle/glucose oxidase anode. Redox probe [Fe(CN)6]3- entrapped inside porous Fe3O4 nanoparticles by DNA. However, in the presence target miRNA, hybridization reaction occurs between DNA, which initiates release [Fe(CN)6]3-. Moreover, duplex specific nuclease further employed to trigger recycling...
Bright carbon nanodot–DNA nanoconjugates are prepared for target miRNA induced concatenated hybridization chain reaction.
MicroRNAs (MiRNAs) are valuable biomarkers for the diagnosis and prognosis of diseases. The development reliable assays is an urgent pursuit. We herein fabricate a novel electrochemical sensing strategy based on conformation transitions DNA nanostructures click chemistry. Duplex-specific nuclease (DSN)-catalyzed reaction first used disintegration triangular pyramid frustum (DNA TPF). A triangle formed, which in turn assists strain-promoted alkyne-azide cycloaddition (SPAAC) to localize...
Highly sensitive and selective detection of microRNA (miRNA) is becoming more important in the discovery, diagnosis, prognosis various diseases. Herein, we develop a three-dimensional DNA nanostructure based electrochemical platform for duplicate miRNA amplified by nicking endonuclease. Target first helps construction three-way junction structures on surfaces gold nanoparticles. After endonuclease-powered cleavage reactions, single-stranded DNAs labeled with species are released. These...