A5003567441- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Parvovirus B19 Infection Studies
- Immune Cell Function and Interaction
- Genetic Neurodegenerative Diseases
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Neuroscience of respiration and sleep
- Lymphoma Diagnosis and Treatment
- HIV Research and Treatment
- Drug Transport and Resistance Mechanisms
- Neuroendocrine regulation and behavior
- Systemic Sclerosis and Related Diseases
- Neuroscience and Neuropharmacology Research
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Phytochemistry and Biological Activities
- Neurological and metabolic disorders
- Immune Response and Inflammation
- RNA modifications and cancer
- Parkinson's Disease and Spinal Disorders
- Biological and pharmacological studies of plants
- Nutrition and Health in Aging
- Therapeutic Uses of Natural Elements
University of California, Davis
2021-2025
Nagoya University
2019-2024
Hyoja Geriatric Hospital
1992-2000
Tokyo National Hospital
1998
Teikyo University
1998
Kobe University
1998
Nagoya City University
1996-1997
Institute of Chemistry
1995
Peking University
1995
North Carolina State University
1995
Enhancers are a crucial regulator of differential gene expression programs. the cis-regulatory sequences determining target genes’ spatiotemporal and quantitative expression. Here, we show that Kaposi’s sarcoma-associated herpesvirus (KSHV) terminal repeats fulfill enhancer definition for KSHV inducible promoters. The is occupied by latency-associated nuclear antigen (LANA) its interacting proteins, such as CHD4. Neighboring repeat (TR) fragments to lytic promoters drastically enhanced...
Sensing of pathogens’ components is a fundamental cellular immune response. Pathogens have therefore evolved strategies to evade such responses. KSHV LANA multifunctional protein and plays an essential role in maintaining the latent infection by tethering viral genomic DNA host chromosome. We adopted inducible knockdown approach found that depletion induced rapid degradation DNA, which mediated innate sensors autophagy pathway. These observations suggest may play hiding episome from sensors....
Kaposi sarcoma-associated herpesvirus (KSHV) establishes a latent infection in the cell nucleus, but where KSHV episomal genomes are tethered and mechanisms underlying lytic reactivation unclear. Here, we study nuclear microenvironment of episomes show that latency-lytic replication switch is regulated via viral long non-coding (lnc)RNA-CHD4 (chromodomain helicase DNA binding protein 4) interaction. localize with CHD4 ADNP proteins, components cellular ChAHP complex. The proteins occupy...
Epstein-Barr virus (EBV) is a ubiquitous human lymphotropic herpesvirus that causally associated with several malignancies. In addition to latent factors, lytic replication contributes cancer development. this study, we examined whether the gene BNRF1, which conserved among gamma-herpesviruses, has an important role in lymphomagenesis. We found lymphoblastoid cell lines (LCLs) established by BNRF1-knockout EBV exhibited remarkably lower pathogenicity mice xenograft model than LCLs produced...
The 3D architecture of chromatin allows for efficient arrangement, expression, and replication genetic material. genomes all organisms studied to date have been found be organized through some form tiered domain structures.
Viral infection induces dynamic changes in transcriptional profiles. Virus-induced and antiviral responses are intertwined during the infection. Epstein-Barr virus (EBV) is a human gammaherpesvirus that provides model of herpesvirus latency. To measure transcriptome establishment EBV latency, we infected EBV-negative Akata cells with EBV-EGFP performed sequencing (RNA-seq) at 0, 2, 4, 7, 10, 14 days after We found transient downregulation mitotic division-related genes, reflecting...
Hypersomnia occurs frequently in patients with myotonic dystrophy (MyD). We performed a quantitative immunohistochemical study of serotonin (5—HT) containing neurons linked to hypersomnia the dorsal raphe nucleus (DRN) and superior central (SCN) 8 MyD, 5 whom showed hypersomnia, 12 age-matched controls. The densities 5—HT DRN SCN were significantly lower MyD than without These data suggest that loss is associated presence MyD.
Kaposi’s sarcoma-associated herpesvirus (KSHV) is an oncogenic double-stranded DNA virus and the etiologic agent of sarcoma hyperinflammatory lymphoproliferative disorders. Understanding mechanism by which KSHV increases infected cell population crucial for curing KSHV-associated diseases. Using scRNA-seq, we demonstrate that preferentially infects CD14 + monocytes, sustains viral lytic replication through interleukin-6 (vIL-6), activates STAT1 3, induces inflammatory gene expression...
We investigated the effects of dietary diosgenin (Dio), a plant-derived sapogenin, on indomethacin (Indo)-induced intestinal inflammation and alterations in bile secretion rats. In anesthetized rats, secretion, inflammation, blood chemistry were assessed 3 days after two subcutaneous injections Indo given 24 h apart. Dio (> 80 mg.kg-1.day-1) pretreatment significantly inhibited weight food intake decreases inflammation. This protective effect was confirmed by examination gross histological...
Abstract Kaposi’s sarcoma-associated herpesvirus (KSHV) is the causative agent of sarcoma (KS) and two human lymphoproliferative diseases: primary effusion lymphoma AIDS-related multicentric Castleman’s disease. KSHV-encoded latency-associated nuclear antigen (LANA) expressed in KSHV-infected cancer cells responsible for maintaining viral genomes infected cells. Thus, LANA an attractive target therapeutic intervention KSHV-associated diseases. Here, we devised a gene therapy vector using...
Abstract Kaposi’s sarcoma-associated herpesvirus (KSHV) genome contains a terminal repeats (TR) sequence. Previous studies demonstrated that KSHV TR functions as gene enhancer for inducible lytic promoters. Gene enhancers anchor bromodomain-containing protein 4 (BRD4) at specific genomic region, where BRD4 interacts flexibly with transcription-related proteins through its intrinsically disordered domain and exerts transcription regulatory function. Here, we generated recombinant reduced copy...
To clarify the possible relation between extent of involvement catecholaminergic neurons and presence alveolar hypoventilation in patients with myotonic dystrophy (MyD).Respiratory insufficiency has been reported frequently MyD patients. Recent data support hypothesis that this respiratory failure results from a primary dysfunction CNS.The authors performed quantitative immunoreactive study tyrosine hydroxylase (TH+) linked to dorsal central medullary nucleus (DCMN), ventral (VCMN),...
Temporally controlled gene expression is necessary for the propagation of herpesviruses. To achieve this, herpesviruses encode several transcriptional regulators. In Epstein-Barr virus, BcRF1 associates with five viral proteins (BDLF4, BGLF3, BFRF2, BVLF1, and BDLF3.5) to form late (L) regulatory complex, which called preinitiation complex (vPIC), on TATT-containing promoters. However, regulation vPIC has been largely unexplored. this study, we performed two screens using a kinase inhibitor...
Abstract Background Viruses must adapt to the environment of their host cells establish infection and persist. Diverse mammalian cells, including virus-infected release extracellular vesicles such as exosomes containing proteins miRNAs, use these mediate intercellular communication. However, roles in viral remain unclear. Results We screened identify those responsible for exosome-mediated enhancement Epstein–Barr virus (EBV) infection. identified BGLF2 protein encapsulated exosomes, which...
Respiratory insufficiency occurs frequently in patients with myotonic dystrophy (MyD). We have performed a quantitative study of neurons linked to respiratory function the dorsal central medullary nucleus (DCMN), ventral (VCMN), and subtrigeminal (SMN) seven MyD eight age-matched controls. Alveolar hypoventilation type occurred three but not remaining or The densities DCMN, VCMN, SMN were significantly lower than without These data suggest neuronal loss is associated presence may be an...
Kaposi sarcoma-associated herpesvirus (KSHV) inflammatory cytokine syndrome (KICS) is a newly described chronic disease condition caused by KSHV infection and characterized high viral load sustained elevations of serum KSHV-encoded IL-6 (vIL-6) human (hIL-6). KICS has significant immortality greater risks other complications, including malignancies. Although prolonged vIL-6 exposure persistent expected to have key roles in subsequent development, the biological effects remain elusive. Using...
The objective of this study was to characterize the bile secretion, including composition biliary acids, salt pool size, and transcytotic vesicle transport, in a rat model subacute intestinal inflammation induced by indomethacin. Indomethacin treatment significantly decreased acid-independent flow secretion acid cholesterol, while increasing phospholipid output vivo. Although indomethacin did not change size vivo, alpha- beta-muricholic acids were deceased hyodeoxycholic deoxycholic...
Kaposi sarcoma-associated herpesvirus (KSHV) inflammatory cytokine syndrome (KICS) is a newly described chronic disease condition caused by KSHV infection and characterized high viral load sustained elevations of serum KSHV-encoded IL-6 (vIL-6) human (hIL-6). KICS has significant immortality possesses greater risks having other complications, which include malignancies. Although prolonged vIL-6 exposure persistent expected to have key roles in subsequent development, the biological effects...