A5083203391- Mechanisms of cancer metastasis
- Cancer Research and Treatments
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Kruppel-like factors research
- Ferroptosis and cancer prognosis
- RNA Research and Splicing
- Chemical Reactions and Isotopes
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Cancer-related gene regulation
- Colorectal Cancer Treatments and Studies
- HER2/EGFR in Cancer Research
- Cancer Genomics and Diagnostics
- Apelin-related biomedical research
- Renal and related cancers
- Fibroblast Growth Factor Research
- Pancreatitis Pathology and Treatment
- Genetic factors in colorectal cancer
- Protein Tyrosine Phosphatases
- T-cell and B-cell Immunology
- Wnt/β-catenin signaling in development and cancer
- Breast Cancer Treatment Studies
University of Alabama at Birmingham
2008-2024
Czech Academy of Sciences, Institute of Microbiology
2003-2011
University of Florida
2004
University of Alabama
2004
Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal human malignancies, with an overall 5-year survival rate <5%. Genetic analysis PDAC patient samples has shown that specific disease-associated mutations are correlated histologically defined stages neoplastic progression in epithelium. Activating KRAS almost uniformly present early-stage disease, subsequent inactivating p16INK4A, p53, and SMAD4 occurring more advanced lesions. In this study, we have tested...
Monoclonal antibodies (mAb) to epidermal growth factor receptor (EGFr) inhibit tumor cell proliferation and enhance cytotoxicity of chemotherapeutic agents. The purpose this study was investigate the interaction anti-EGFr antibody C225 combined with radiotherapy (RT) on EGFr expressing A431 human epidermoid cancer cells.Cell proliferation, apoptosis, expression phosphorylation, clonogenic survival were assayed in vitro. inhibition immunohistochemistry analysis apoptosis assessed vivo.C225...
Thyroid receptor‐interacting protein 13 (TRIP13), a of the AAA‐ATPase family, is upregulated in various human cancers, including colorectal cancer (CRC). This study focused on inhibition TRIP13‐induced CRC progression and signalling by DCZ0415, small molecule targeting TRIP13. It demonstrated potent antitumour activity TRIP13‐deregulated cell lines, regardless their p53, KRAS, BRAF, epidermal growth factor receptor or microsatellite instability status. The treatment cells with DCZ0415...
Abstract Background Treatment with regorafenib, a multiple-kinase inhibitor, to manage metastatic colorectal cancers (mCRCs) shows modest improvement in overall survival but is associated severe toxicities. Thus, reduce regorafenib-induced toxicity, we used regorafenib at low concentration along dual JAK/HDAC small-molecule inhibitor (JAK/HDACi) leverage the advantages of both JAK and HDAC inhibition enhance antitumor activity. The therapeutic efficacy safety combination treatment was...
Gene knockout experiments in mice have suggested a hierarchical model of early B cell commitment wherein E2A proteins (E47 and E12) activate factor (Ebf1), which turn activates expression the factor, Pax5. In IL-7 receptor alpha (IL-7Ralpha) mice, development is blocked before B-lineage at prepro-B stage adult animals. IL-7Ralpha(-/-) cells, E47 expressed yet insufficient to transcriptionally putative downstream target gene, Ebf1. this study, we show that further increases cells fails Ebf1,...
The identification of colorectal cancer (CRC) molecular targets is needed for the development drugs that improve patient survival. We investigated functional role phosphoribosylaminoimidazole carboxylase, succinocarboxamide synthetase (PAICS), a de novo purine biosynthetic enzyme involved in DNA synthesis, CRC progression and metastasis by using cell animal models. Its clinical utility was assessed human samples. expression PAICS regulated miR-128 transcriptionally activated Myc cells....
Overexpression of TRIP13, a member the AAA‐ATPase family, is linked with various cancers, but its role in metastasis unknown colorectal cancer (CRC). In current study, we investigated TRIP13 experimental and involvement regulation WNT/β‐catenin EGFR signaling pathways. Evaluation formalin‐fixed paraffin‐embedded (FFPE) frozen tissues adenomas CRCs, along their corresponding normal samples, showed that was gradually increased phenotypic expression from adenoma to carcinoma overexpression CRCs...
Deposition, remodeling, and signaling of the extracellular matrix facilitate tumor growth metastasis. Here, we demonstrated that an enzyme, collagen prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), which is involved in synthesis deposition, had elevated expression colorectal cancers (CRCs) as compared to normal colonic tissues. The P4HA1 CRCs was independent patient's age, race/ethnicity, gender, pathologic stage grade, location, microsatellite instability (MSI) p53 status. By modulating...
Triple negative breast cancers (TNBCs) are molecularly heterogeneous, and the link between their aggressiveness with African ancestry is not established. We investigated primary TNBCs for gene expression among self-reported race (SRR) groups of American (AA,
: Triple-negative breast cancer (TNBC) patients of various ethnic groups often have discrete clinical presentations and outcomes. Women African descent a disproportionately higher chance developing TNBCs. The aim the current study was to establish transcriptome TNBCs from Kenyan (KE) women Bantu origin compare it those African-Americans (AA) Caucasians (CA) for identifying KE TNBC-specific molecular determinants progression potential biomarkers Pathology-confirmed TNBC tissues (n = 15) age...
Thyroid hormone receptor-interacting protein 13 (TRIP13) is involved in cancer progression, but its role pancreatic ductal adenocarcinoma (PDAC) unknown. Thus, we assessed the expression, functional role, and mechanism of action TRIP13 PDAC. We further examined efficacy inhibitor, DCZ0415, alone or combination with gemcitabine on malignant phenotypes, tumor immune response. found that was overexpressed human PDACs relative to corresponding normal tissues. knockdown treatment PDAC cells...
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer with extremely poor prognosis. There urgent need to identify new therapeutic targets and also understand the mechanism of PDAC progression that leads aggressiveness disease. To find targets, we analyzed data related transcriptome sequencing found overexpression de novo purine metabolic enzyme phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS). Immunohistochemical analysis tissues showed high expression PAICS...
Abstract In the US, majority of cancer samples analyzed are from white people, leading to biases in racial and ethnic treatment outcomes. Colorectal (CRC) incidence mortality rates high Alabama African Americans (AAs) Oklahoma American Indians (AIs). We hypothesized that differences between groups may partially explain these disparities. Thus, we compared transcriptomic profiles CRCs AAs, AIs, people both states. Compared AAs showed (a) higher expression cytokines vesicle trafficking toward...
Colorectal cancer (CRC) is the fifth most diagnosed in Sub-Saharan Africa. In Kenya, CRC incidence rates tripled from 1997 to 2017. Moi Teaching and Referral Hospital, University, there has been an increase cases, notably for younger patients. A suggested pathobiology this gut microbiome dysbiosis. Since, Kenyan patient population, studies are rare, a need better understanding of how dysbiosis influences epidemiology Kenya. single-center study, focus was on profiling patients healthy...
Because survival of patients with metastatic colorectal cancer remain poor, there is an urgent need to identify potential novel druggable targets that are associated progression. One such target, basic leucine zipper and W2 domains 2 (BZW2), involved in regulation protein translation, its overexpression human malignancy. Thus, we investigated the expression BZW2, assessed role activation WNT/β-catenin signaling, identified downstream molecules, demonstrated involvement metastasis cancer. In...
Rare diseases are underrepresented in biomedical research, leading to insufficient awareness. Zhu-Tokita-Takenouchi-Kim (ZTTK) syndrome is a rare disease caused by genetic alterations that result heterozygous loss-of-function of SON. While ZTTK patients suffer from numerous symptoms, the lack model organisms hampers our understanding SON and this complex syndrome. Here, we developed Son haploinsufficiency (Son+/−) mice as identified indispensable roles organ development hematopoiesis. Son+/−...
The cyclin D1/bcl-1 proto-oncogene is one of a series genes encoding proteins which regulate the cell cycle and are involved in multistep process tumorigenesis. Translocation D1 common event B lymphoma, amplification occurs breast, esophageal, hepatocellular, head/neck carcinomas. human promoter contains an 18-base pair purine-pyrimidine rich motif with three C·G interruptions. This potential target for purine·purine·pyrimidine triplex formation. We have designed G-rich antiparallel forming...
Temporally controlled expression of genes in transgenic mice has advanced our understanding many physiological processes. One the more common modes acutely altering gene levels involves doxycycline‐regulated “tet‐on/tet‐off” systems. There been concern that administration doxycycline itself might compromise aspects wound repair. Here we report oral ad libitum (2 mg/ml drinking water) to does not alter dermal or epidermal healing. The healing both full‐ and partial‐thickness skin wounds...
PurposeAlthough there is an established role for microbiome dysbiosis in the pathobiology of colorectal cancer (CRC), CRC patients various race/ethnicities demonstrate distinct clinical behaviors. Thus, we investigated Egyptian, African American (AA), and European (EA) patients.Patients methodsCRCs their corresponding normal tissues from Egyptian (n = 17) Alexandria University Hospital, Egypt, AA 18) EA 19) at Alabama Birmingham were collected. DNA was isolated frozen tissues, composition...