A5008120184- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Medical Imaging Techniques and Applications
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Pancreatic and Hepatic Oncology Research
- Radiopharmaceutical Chemistry and Applications
- CAR-T cell therapy research
- Ultrasound and Hyperthermia Applications
- Herpesvirus Infections and Treatments
- Nanoplatforms for cancer theranostics
- Cytokine Signaling Pathways and Interactions
- Galectins and Cancer Biology
- Dysphagia Assessment and Management
- Estrogen and related hormone effects
- Chemokine receptors and signaling
- Advanced MRI Techniques and Applications
- Angiogenesis and VEGF in Cancer
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- MRI in cancer diagnosis
- Pediatric health and respiratory diseases
- Photodynamic Therapy Research Studies
- Trace Elements in Health
- Thallium and Germanium Studies
University of Alabama at Birmingham
2011-2022
St. Vincent's Birmingham
2014
Pediatric Infectious Diseases Society
2006
University of Pennsylvania
1995
PURPOSE: To use the eye position recordings of observers as they read chest images to advance understanding mechanism "satisfaction search," a phenomenon in which detection one abnormality interferes with other abnormalities. MATERIALS AND METHODS: Eight radiologists examined that contained simulated pulmonary nodules and native Accuracy detecting abnormalities position, gaze duration, total search time were recorded. RESULTS: Nodule detectability was lower on abnormality-containing than it...
The study goal was to examine the relationship between nab-paclitaxel delivery and SPARC (secreted protein acidic rich in cysteine) expression pancreatic tumor xenografts determine antistromal effect of nab-paclitaxel, which may affect vascular perfusion. SPARC-positive -negative mice bearing Panc02 (n = 5-6/group) were injected with IRDye 800CW (IR800)-labeled nab-paclitaxel. After 24 hours, tumors collected stained DL650-labeled anti-SPARC antibody, correlation distributions examined....
Chronic obstructive pulmonary disease (COPD) is the third leading cause of death in US. The majority COPD patients have symptoms chronic bronchitis, which lacks specific therapies. A major impediment to therapeutic development has been absence animal models that recapitulate key clinical and pathologic features human disease. Ferrets are well suited for investigation significance respiratory diseases, given prior data indicating similarities airway physiology submucosal gland distribution....
Objectives To determine whether volumetric contrast‐enhanced ultrasound (US) imaging has the potential to monitor changes in renal perfusion after vascular injury. Methods Volumetric US uses a series of planar image acquisitions, capturing nonlinear second harmonic signal from microbubble contrast agents flowing vasculature. Tissue parameters (peak intensity [I PK ], time peak [T wash‐in rate [WIR], and area under curve [AUC]) were derived time‐intensity data collected during vitro flow...
Abstract The selectins and their ligands mediate leukocyte rolling on endothelial cells, the initial step in emigration cascade leading to infiltration of tissue. These adhesion molecules have been shown be key promoters acute events; however, roles development long-term inflammatory responses, including those that occur during chronic diseases such as systemic lupus erythematosus, are unclear. To assess participation P-selectin disorders, we studied progression erythematosus-like disease...
The selectins, along with very late antigen-4 and CD44, have been implicated in mediating leukocyte rolling interactions that lead to joint recruitment inflammation during the pathogenesis of rheumatoid arthritis. Previously, we showed P-selectin deficiency mice resulted accelerated onset murine collagen-immunized arthritis model. Here, report deficient either E-selectin or (E/P-selectin mutant) also exhibit development compared wild type CIA model, suggesting these adhesion molecules...
Abstract The goal of the study was to assess efficacy combined extracellular matrix metalloprotease inducer (EMMPRIN)- and death receptor 5 (DR5)-targeted therapy for pancreatic adenocarcinoma in orthotopic mouse models with multimodal imaging. Cytotoxicity anti-EMMPRIN antibody anti-DR5 (TRA-8) MIA PaCa-2 PANC-1 cell lines measured by ATPlite assay vitro. distributions Cy5.5-labeled TRA-8 Cy3-labeled 2 were analyzed fluorescence imaging Groups 1 12 severe immunodeficient mice bearing...
TRA-8, a monoclonal antibody targeting death receptor, has demonstrated high therapeutic effect for triple negative breast cancer (TNBC) in preclinical models. Tamoxifen, the standard of care ERα-positive cancer, induces apoptosis via ERβ, which commonly presents TNBC cells. The current study investigates combination effects TRA-8 and tamoxifen TNBC. In vitro assays were implemented with two ERβ-positive cell lines, SUM159 2LMP, vivo therapy studies followed using orthotopic tumor mouse IC50...
The objective of this study was to evaluate extracellular matrix metalloproteinase (EMMPRIN) as a novel target in orthotopic pancreatic cancer murine models. MIA PaCa-2 human tumor cells were implanted groups 1 and 3-7, whereas EMMPRIN knockdown group 2. Dosing with anti-EMMPRIN antibody started immediately after implantation for 1-3 (residual model) at 21 days cell 4-7 (established model). Groups 3, 5, 7 treated anti-EMMRPIN (0.2-1.0 mg) twice weekly 2-3 weeks, the other served control. In...
Purpose: To evaluate binding of P-selectin targeted microbubbles (MB) in tumor vasculature; a whole-body imaging and biodistribution study was performed bearing mouse model.Methods: Antibodies were radiolabeled with Tc-99 m using the HYNIC method. labeled anti-P-selectin antibodies avidin-bound to lipid-shelled, perfluorocarbon gas-filled MB intravenously injected into mice MDA-MB-231 breast tumors. Whole-body at 5 min (n = 12) 60 4) gamma counter. m-labeled IgG bound IgG-control-MB group 12...
ABSTRACT Previous studies have described in vitro serial passage of a Δγ 1 34.5 herpes simplex virus type (HSV-1) strain SK-N-SH neuroblastoma cells and selection mutants that acquired the ability to infect replicate this previously nonpermissive cell line. Here we describe mutant HSV-1 by vivo passage, which prolongs survival two separate experimental murine brain tumor models. Two conditionally replication-competent viruses, M002, expresses interleukin-12, its parent virus, R3659, were...
Purpose To assess the early response of triple-negative breast-cancer (TNBC) following TRA-8 and carboplatin therapy using DWI MRS in 2LMP SUM159 mouse models. Materials Methods Four groups (n = 5/group) each model were untreated or treated with carboplatin, TRA-8, combination, respectively. applied on 0, 3, 7 days after initiation, all tumors collected thereafter for terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining. The changes intratumoral apparent...
DNA damage repair and tumor hypoxia contribute to intratumoral cellular molecular heterogeneity affect radiation response. The goal of this study is investigate anti-HER2-induced radiosensitization the microenvironment enhance fractionated radiotherapy in models HER2+ breast cancer. This monitored through vitro vivo studies phosphorylated γ-H2AX, [18F]-fluoromisonidazole (FMISO)-PET, transcriptomic analysis. In vitro, cancer cell lines were treated with trastuzumab prior double-strand breaks...
Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) has been limitedly used for orthotopic pancreatic tumor xenografts due to severe respiratory motion artifact in the abdominal area. Orthotopic models offer advantages over subcutaneous ones, because those can reflect primary microenvironment affecting blood supply, neovascularization, and cell invasion. We have recently established a protocol of DCE-MRI mouse by securing tumors with an orthogonally bent plastic board prevent...
Using a series of potential biomarkers relevant to mechanisms protein synthesis, we observed that estrogen receptor (ER)-positive breast tumor cells exist in two distinct yet interconvertible phenotypic states (of roughly equal proportion) which differ the degree differentiation and use IRES-mediated translation. Nascently translated IGF1R cytoplasm positively correlated with IRES activity undifferentiated phenotype, while epitope accessibility RACK1, an integral component 40S ribosomal...
Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) has been limitedly used for orthotopic pancreatic tumor xenografts due to severe respiratory motion artifact in the abdominal area. Orthotopic models offer advantages over subcutaneous ones, because those can reflect primary microenvironment affecting blood supply, neovascularization, and cell invasion. We have recently established a protocol of DCE-MRI mouse by securing tumors with an orthogonally bent plastic board prevent...
COPD patients exhibit reduced CFTR ion transport activity, mucus hypersecretion, and impaired mucociliary clearance (MCC). dysfunction in is associated with chronic bronchitis, but not emphysema. But, research has been limited by the absence of a viable animal model that exhibits bronchitis. Here, we developed ferret bronchitis via cigarette smoke exposure. (WCS) exposure for 6 months. In vivo activity was measured Nasal Potential Difference (NPD) MCC assayed radiographic Tc99. Six months...