A5100718134- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- DNA and Nucleic Acid Chemistry
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- MicroRNA in disease regulation
- Immune cells in cancer
- RNA modifications and cancer
- Genomics, phytochemicals, and oxidative stress
- Nanoplatforms for cancer theranostics
- Biosensors and Analytical Detection
- Cancer-related molecular mechanisms research
- Protease and Inhibitor Mechanisms
- Calpain Protease Function and Regulation
- Digestive system and related health
- Viral gastroenteritis research and epidemiology
- Nanopore and Nanochannel Transport Studies
- Plant Virus Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Trace Elements in Health
- Plant and Fungal Interactions Research
- Biomarkers in Disease Mechanisms
- Mosquito-borne diseases and control
- Liver physiology and pathology
Guangxi Medical University
2022-2025
Henan Agricultural University
2025
Hubei University of Chinese Medicine
2019-2024
Wake Forest University
2024
Affiliated Hospital of Jining Medical University
2024
Sichuan Normal University
2024
Nanjing University of Chinese Medicine
2024
Sichuan Academy of Traditional Chinese Medicine
2023
Loyola University Chicago
2023
Xijing Hospital
2023
Since April 2010, a severe outbreak of duck viral infection, with egg drop, feed uptake decline and ovary-oviduct disease, has spread around the major duck-producing regions in China. A new virus, named BYD was isolated different areas, similar disease reproduced healthy egg-producing ducks, infecting virus. The virus re-isolated from affected ducks replicated well primary embryo fibroblasts Vero cells, causing cytopathic effect. identified as an enveloped positive-stranded RNA size...
Accurately assaying tumor-derived circulating extracellular vesicles (EVs) is fundamental in noninvasive cancer diagnosis and therapeutic monitoring but limited by challenges efficient EV isolation profiling. Here, we report a bioinspired buoyancy-driven metal-organic framework (MOF) corona that leverages on-bubble coordination dual-encoded surface-enhanced Raman scattering (SERS) nanotags to streamline rapid ultrasensitive profiling of plasma EVs single assay for diagnostics. This...
Ovarian carcinoma is caused by multiple factors, but its etiology associated with microbes and infection unknown. Using 16S rRNA high-throughput sequencing methods, the diversity composition of microbiota from ovarian cancer tissues (25 samples) normal distal fallopian tube were analyzed. High-throughput showed that richness indexes significantly decreased in compared to tubes. The ratio two phyla for Proteobacteria/Firmicutes was notably increased cancer, which revealed microbial change...
Exosomal microRNAs are essential in intercellular communications and disease progression, yet it remains challenging to quantify the expression level due their small size low abundance blood. Here, we report a "sandwich" electrochemical exosomal microRNA sensor (SEEmiR) detect target with high sensitivity specificity. In SEEmiR, neutrally charged peptide nucleic acid (PNA) enables kinetically favorable hybridization relative negatively DNA, particularly short sequence (10 nt). More...
DNA self-assembly has created various nanostructured probes deployed in biosensors, whereas their direct charge contribution to sensitive bioassays remains elusive. Here, we report a supercharged tetrahedral nanolabel-based electrochemical (eTDN) sensor for ultrasensitive detection of exosomal microRNAs (Exo-miRs). By using an "assembly before testing" strategy, there is high-efficiency recognition between the target Exo-miR and self-assembled TDN probe homogenous solution relative...
Exosomes are emerging as promising biomarkers for cancer diagnosis, yet sensitive and accurate quantification of tumor-derived exosomes remains a challenge. Here, we report an ultrasensitive specific exosome sensor (NPExo) that initially leverages hierarchical nanostructuring array primer exchange reaction (PER) quantitation cancerous exosomes. This NPExo uses high-curvature (bottom) fabricated by single-step electrodeposition to enhance capturing the target The immuno-captured thus provides...
Abstract Solid bubbles have expanded the SERS assay toolbox, but their detection performance in biofluids is still hampered by irrational design of plasmonic sensing interface. A bubble aggregate‐driven DNA‐encoded reported here that enables simultaneous, ultrasensitive, and specific multiple miRNAs blood samples for accurate cancer diagnosis. In this assay, buoyancy allows them to self‐aggregate at a droplet apex reconfiguration, form single‐layer aggregates with nanogaps, prevent coffee...
Efficient profiling of circulating extracellular vesicles (EVs) benefits noninvasive cancer diagnosis and therapeutic monitoring, but is technically hampered by tedious isolation, multistep washing, poor sensitivity. Here, we report multifunctional bubbles that enable self-separation, wash-free, single-step, ultrasensitive EV surface markers in plasma samples for early treatment monitoring lung cancer. In this assay, the buoyancy-dominated bubble electric field-resistant, allowing...
Duck egg-drop syndrome virus (DEDSV) is a newly emerging pathogenic flavivirus causing avian diseases in China. The infection occurs laying ducks characterized by severe drop egg production with fatality rate of 5–15 %. was found to be most closely related Tembusu (TMUV), an isolate from mosquitoes South-east Asia. Here, we have sequenced and the full-length genomes seven DEDSV strains, including 5′- 3′-non-coding regions (NCRs). We also report for first time ORF sequences TMUV Sitiawan...
Nonhepatic delivery of small interfering RNAs (siRNAs) remains a challenge for development RNA interference-based therapeutics. We report noncationic vector wherein linear poly(ethylene glycol) (PEG), polymer generally considered as inert and safe biologically but ineffective vector, is transformed into bottlebrush architecture. This topology provides covalently embedded siRNA with augmented nuclease stability cellular uptake. Consisting almost entirely PEG siRNA, the conjugates exhibit...
Oligonucleotide-based materials such as spherical nucleic acid (SNA) have been reported to exhibit improved penetration through the epidermis and dermis of skin upon topical application. Herein, we report a self-assembled, skin-depigmenting SNA structure, which is based bifunctional oligonucleotide amphiphile containing an antisense tyrosinase inhibitor prodrug. The two components work synergistically increase cellular uptake, enhance drug solubility, promote penetration. particles were...
Interfacial DNA self-assembly is fundamental to solid nucleic acid biosensors, whereas how improve the signal-to-noise ratio has always been a challenge, especially in charge-based electrochemical sensors because of large noise from negatively charged capture probes. Here, we report framework-reversed signal-gain strategy through background-to-signal transition for ultrasensitive and highly specific electrical detection microRNAs (miRNAs) blood. By using model enzyme-catalyzed deposition...
Notch signaling plays an emerging role in the regulation of immune cell development and function during inflammatory response. Activation ras homolog gene family member A/Rho‐associated protein kinase (ROCK) pathway promotes leukocyte accumulation tissue injury. However, it remains unknown whether regulates A/ROCK–mediated responses liver ischemia reperfusion (IR) This study investigated intracellular pathways regulated by receptors IR‐stressed vitro . In a mouse model IR‐induced injury, we...
Exosome-associated liquid biopsies are hampered by challenges in the exosomal quantification and phenotyping. Here, we present a bioinspired exosome-activated DNA molecular machine (ExoADM) with multivalent cyclic amplification that enables highly sensitive detection phenotyping of circulating exosomes. ExoADM harbors two (an exposed hidden) toehold domains actuate sequential branch migration recycling response to surface markers. Importantly, this self-powered achieves high sensitivity (33...
PEGylation of an oligonucleotide using a brush polymer can improve its biopharmaceutical characteristics, including enzymatic stability and biodistribution. Herein, we quantitatively explore the nuclease accessibility nucleic acid as function "depth" toward backbone polymer. It is found that protein decreases nucleotide located closer to backbone. Thus, by moving conjugation point from terminus strand internal position, much smaller brushes be used achieve same level steric shielding. This...
VTo investigate the effect of nuclear transcription factor Nrf2 on Ferroportin (FPN) in prostate cancer cells, and regulation mechanisms FPN cell viability, migration apoptosis cells.Empty vectors, pEGFPC1-Nrf2, pEGFPC1-FPN, Si-FPN Si-Nrf2 were transfected into line PC3. The expression mRNA protein measured by real time-PCR (RT-PCR) western blot. Cell migration, cycle tested CCK-8 assay, wound healing flow cytometry, respectively. interaction between was confirmed chromatin...
Abstract This study aims to the effects of intra-nuclear lncRNA MEG3 on progression prostate cancer and underlying mechanisms. Expressions relative molecules were detected by Quantitative real time PCR (qRT-PCR) western blot. Chromatin immunoprecipitation RNA (RIP) assays used evaluate interaction between MEG3, histone methyltransferase EZH2 Engrailed-2 (EN2). The impacts viability, proliferation invasion cells (PC3) evaluated methyl thiazolyl tetrazolium, colony formation transwell assays,...
MicroRNA (MiRNA)-based noninvasive diagnostics are hampered by the challenge in quantification of circulating miRNAs using a general strategy. Here, we present base-stacking effect-mediated ultrasensitive electrochemical miRNA sensor (BSee-miR) with universal sandwich configuration. In BSee-miR, short DNA probe (10 nucleotides) self-assembled on gold electrode surface could effectively capture target synergizing another sequence based coaxial base-stacking, which rivals fully complementary...
Abstract Background Perihematomal edema (PHE) after post-intracerebral hemorrhage (ICH) has complex pathophysiological mechanisms that are poorly understood. The complicated immune response in the post-ICH brain constitutes a crucial component of PHE pathophysiology. In this study, we aimed to characterize transcriptional profiles cell populations human tissue and explore microscopic differences between different types cells. Methods 9 patients with basal ganglia intracerebral (hematoma...
The PI3K/Akt/mTOR axis in ovarian cancer is frequently activated and implicated tumorigenesis. Specific targeting of this pathway therefore an attractive therapeutic approach for cancer. However, cells are resistant to PP242, a dual inhibitor mTORC1 mTORC2. Interestingly, blockage GLS1 with selective inhibitor, CB839, or siRNA dramatically sensitized the PP242-induced cell death, as evident from increased PARP cleavage. anti-cancer activity CB-839 PP242 was abrogated by addition TCA cycle...