A5112039916- Hearing, Cochlea, Tinnitus, Genetics
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- Organ Transplantation Techniques and Outcomes
- Liver Disease Diagnosis and Treatment
- Vestibular and auditory disorders
- Circular RNAs in diseases
- Ear Surgery and Otitis Media
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Aquaculture disease management and microbiota
- Endoplasmic Reticulum Stress and Disease
- Virus-based gene therapy research
- Autophagy in Disease and Therapy
- Immune cells in cancer
- Hippo pathway signaling and YAP/TAZ
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Invertebrate Immune Response Mechanisms
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Hearing Loss and Rehabilitation
- interferon and immune responses
- Liver Disease and Transplantation
Nanjing Drum Tower Hospital
2016-2025
Zhongda Hospital Southeast University
2023-2025
Jiangjin Central Hospital
2025
Chongqing University
2025
Jiangsu Province Hospital
2013-2024
Affiliated Hospital of Jiangsu University
2021-2024
Nanjing Medical University
2015-2024
Xi’an University
2018-2024
East China Normal University
2023-2024
Shanghai Electric (China)
2024
Topological photonic states, inspired by robust chiral edge states in topological insulators, have recently been demonstrated a few systems, including an array of coupled on-chip ring resonators at communication wavelengths. However, the intrinsic difference between electrons and photons determines that protection time-reversal-invariant systems does not share same robustness as its counterpart electronic insulators. Here, designer surface plasmon platform consisting tunable metallic...
Aminoglycosides are toxic to sensory hair cells (HCs). Macroautophagy/autophagy is an essential and highly conserved self-digestion pathway that plays important roles in the maintenance of cellular function viability under stress. However, role autophagy aminoglycoside-induced HC injury unknown. Here, we first found activity was significantly increased, including enhanced autophagosome-lysosome fusion, both cochlear HCs HEI-OC-1 after neomycin or gentamicin injury, suggesting might be...
Nonalcoholic steatohepatitis (NASH) is a chronic liver disease associated with dysregulation of metabolism and inflammation. G-protein coupled bile acid receptor 1 (TGR5) cell surface that involved in multiple metabolic pathways. However, the functions TGR5 regulating macrophage innate immune activation NASH remain unclear. Here, we found expression was decreased tissues from humans mice NASH. Compared to wild type (WT) mice, TGR5-knockout (TGR5-/-) exhibited exacerbated damage, increased...
Non-alcoholic steatohepatitis (NASH) is associated with the dysregulation of lipid metabolism and hepatic inflammation, though underlying mechanisms remain unclear. We aimed to investigate role X-box binding protein-1 (XBP1) in progression NASH.
Abstract Mutations in OTOFERLIN ( OTOF ) lead to the autosomal recessive deafness 9 (DFNB9). The efficacy of adeno‐associated virus (AAV)‐mediated gene replacement therapy is extensively validated Otof ‐deficient mice. However, clinical safety and AAV‐ not reported. Here, generated using good manufacturing practice its mouse non‐human primates order determine optimal injection dose, volume, administration route for trials. Subsequently, delivered into one cochlea a 5‐year‐old deaf patient...
Oxidative stress-mediated ferroptosis and macrophage-related inflammation play an important role in various liver diseases. Here, we explored if how hepatocyte regulates macrophage stimulator of interferon genes (STING) activation the development spontaneous damage, fibrosis, tumorigenesis.We used a transforming growth factor-beta-activated kinase 1 (TAK1) deficiency-induced model tumorigenesis to investigate its impact on STING signalling. Primary hepatocytes macrophages were for vitro...
Background Pacific white shrimp (Litopenaeus vannamei), the major species of farmed shrimps in world, has been attracting extensive studies, which require more and genome background knowledge. The now available transcriptome data L. vannamei are insufficient for research requirements, have not adequately assembled annotated. Methodology/Principal Findings This is first study that used a next-generation high-throughput DNA sequencing technique, Solexa/Illumina GA II method, to analyze from...
Although the roles of metabolic stress in organ ischemia-reperfusion injury (IRI) have been well recognized, question whether and how these responses regulate innate immune activation against IR remains unclear. In a murine liver partial warm ischemia mode, we showed that prolonged triggered endoplasmic reticulum (ER) response, particularly, activating transcription factor 6 (ATF6) branch, Kupffer cells (KCs) altered their responsiveness Toll-like receptor (TLR) stimulation. Ischemia-primed...
Abstract Atg7 is an indispensable factor that plays a role in canonical nonselective autophagy. Here we show genetic ablation of outer hair cells (OHCs) mice caused stereocilium damage, somatic electromotility disturbances, and presynaptic ribbon degeneration over time, which led to the gradual wholesale loss OHCs subsequent early-onset profound hearing loss. Impaired autophagy disrupted OHC mitochondrial function triggered accumulation dysfunctional mitochondria would otherwise be...
XBP1 modulates the macrophage proinflammatory response, but its function in stimulator of interferon genes (STING) activation and liver fibrosis is unknown. X-box binding protein 1 (XBP1) has been shown to promote nucleotide-binding oligomerization domain, leucine-rich repeat pyrin domain-containing 3 (NLRP3) steatohepatitis. Herein, we aimed explore underlying mechanism regulation STING signalling subsequent NLRP3 during fibrosis.XBP1 expression was measured human fibrotic tissue samples....
Abstract OTOF mutations are the principal causes of auditory neuropathy. There reports on Otof ‐related gene therapy in mice, but there is no preclinical research drug evaluations. Here, Anc80L65 and mouse hair cell‐specific Myo15 promoter (mMyo15) used to selectively effectively deliver human cells mice nonhuman primates evaluate efficacy safety drugs. A new dual‐AAV ‐OTOF‐ hybrid strategy transfer full‐length generated, which can stably restore hearing adult p.Q939*/Q939* with profound...
Stromal cell-derived exosomes have demonstrated their value in the field of biomedical engineering. However, low production and specific requirements different diseases limited practical efficacy these restricted wider applications. Here, we presented a method to culture genetically engineered mesenchymal stromal cells (MSC) that overexpressed hepatocyte growth factor (HGF) microfluidics harvest mass HGF for wound healing. The microfluidic chips were featured with herringbone grooves...
Abstract Background Inner ear supporting cells (SCs) in the neonatal mouse cochlea are a potential source for hair cell (HC) regeneration, but several studies have shown that regeneration ability of SCs decreases dramatically as mice age and lost HCs cannot be regenerated adult mice. To better understand how might used to regenerate HCs, it is important gene expression profile changes at different ages. Methods Here, we Sox2 GFP/+ isolate Sox2+ postnatal day (P)3, P7, P14, P30 via flow...
Abstract Tobacco smoke (TS) is the most important single risk factor for bladder cancer. Epithelial–mesenchymal transition (EMT) a transdifferentiation process, involved in initiation of TS-related Cancer stem cells (CSCs) have an essential role progression many tumors including However, molecular mechanisms TS exposure induced urocystic EMT and acquisition CSCs properties remains undefined. Wnt/ β -catenin pathway critical maintenance CSCs. The aim our present study was to investigate...
Calorie restriction plus dietary advice is suggested as a preventive strategy for individuals with obesity and prediabetes; however, optimal diet still debatable. We aimed to compare the effects of Mediterranean (MD) Chinese diets high or low in plants on body weight glucose homeostasis among high-risk Chinese.In this parallel-arm randomized controlled trial, 253 adults aged 25 60 years mass index ≥ 24.0 kg/m2 fasting blood 5.6 mmol/L were randomly assigned 3 isocaloric-restricted diets: MD...
The nuclear factor-kappa B (NF-κB) pathways play important roles in innate immune responses. IκB is the main cytoplasmic inhibitor of NF-κB. In this study, we identified LvCactus gene from Litopenaeus vannamei, which first cloned homologue subphylum Crustacea. contains six predicted ankyrin repeats, show similarities to those Cactus proteins insects. localizes cytoplasm and interacts with LvDorsal, an L. vannamei Drosophila melanogaster Dorsal belonging class II NF-κB family, prevent its...
ARHGEF6 belongs to the family of guanine nucleotide exchange factors (GEFs) for Rho GTPases, and it specifically activates GTPases CDC42 RAC1. Arhgef6 is X-linked intellectual disability gene also known as XLID46, clinical features patients carrying mutations include and, in some cases, sensorineural hearing loss. act molecular switches many cellular processes. Their activities are regulated by binding or hydrolysis GTP, which facilitated GTPase-activating proteins, respectively. RAC1 have...
Notch signaling plays an emerging role in the regulation of immune cell development and function during inflammatory response. Activation ras homolog gene family member A/Rho‐associated protein kinase (ROCK) pathway promotes leukocyte accumulation tissue injury. However, it remains unknown whether regulates A/ROCK–mediated responses liver ischemia reperfusion (IR) This study investigated intracellular pathways regulated by receptors IR‐stressed vitro . In a mouse model IR‐induced injury, we...