A5028369549- Endoplasmic Reticulum Stress and Disease
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Trace Elements in Health
- Biosensors and Analytical Detection
- Neuroscience and Neuropharmacology Research
- Aquaculture disease management and microbiota
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Genetic and Kidney Cyst Diseases
- Heavy metals in environment
- Neuroinflammation and Neurodegeneration Mechanisms
- Fungal and yeast genetics research
- Proteins in Food Systems
- Neurotransmitter Receptor Influence on Behavior
- RNA regulation and disease
- Autophagy in Disease and Therapy
- Spinal Cord Injury Research
- Protein Kinase Regulation and GTPase Signaling
- Immune Cell Function and Interaction
- Autism Spectrum Disorder Research
- Aluminum toxicity and tolerance in plants and animals
- Obsessive-Compulsive Spectrum Disorders
- Extracellular vesicles in disease
Qingdao Agricultural University
2023-2025
Affiliated Hospital of Nantong University
2024
Nantong University
2024
Nankai University
2005-2024
Chinese Academy of Sciences
2015-2023
Southern Medical University
2023
Institute of Botany
2012-2023
Zhujiang Hospital
2023
Southeast University
2017-2022
State Key Laboratory of Plant Genomics
2021
Abstract The systemic RNAi-defective (SID) transmembrane family member 2 (SIDT2) is a putative nucleic acid channel or transporter that plays essential roles in transport and lipid metabolism. Here, we report the cryo-electron microscopy (EM) structures of human SIDT2, which forms tightly packed dimer with extensive interactions mediated by two previously uncharacterized extracellular/luminal β-strand-rich domains unique domain (TMD). TMD each SIDT2 protomer contains eleven helices (TMs), no...
The neurobiological understanding of obsessive–compulsive disorder (OCD) includes dysregulated frontostriatal circuitry and altered monoamine transmission. Repetitive stereotyped behavior (e.g., grooming), a featured symptom in OCD, has been proposed to be associated with perturbed dopamine (DA) signaling. However, the precise brain circuits participating DA’s control over this behavioral phenotype remain elusive. Here, we identified that DA neurons substantia nigra pars compacta (SNc)...
Purpose: This study aimed to explore the impact that coping styles and social support have on mental health of medical students by constructing a corresponding structural situation model reveals complex relationship between these three factors. In doing so, it seeks help medication better manage problems. Patients Methods: The online was conducted March 6, 2021 May 2021. A total 318 participants from multiple schools were involved. general information questionnaire, simple style...
Abstract The defects in storage proteins secretion the endosperm of transgenic rice seeds often leads to endoplasmic reticulum (ER) stress, which produces floury and shrunken seeds, but mechanism this response remains unclear. We used an iTRAQ-based proteomics analysis ER-stressed due endosperm-specific suppression OsSar1 identify changes protein levels ER stress. stress changed expression 405 seed by >2.0- fold compared with wild-type control. Of these proteins, 140 were upregulated 265...
Endoplasmic reticulum-associated protein degradation (ERAD) plays an important role in endoplasmic reticulum (ER) quality control. To date, little is known about the retrotranslocation machinery plant ERAD pathway. We obtained a DERLIN-like (OsDER1) through SWATH-based quantitative proteomic analysis of ER membrane proteins extracted from ER-stressed rice (Oryza sativa) seeds. OsDER1, homolog yeast and mammal DER1, localized accumulates significantly under stress. Overexpression or...
Abstract Metallochaperones are a unique class of proteins that play crucial roles in metal homoeostasis and detoxification. However, few metallochaperones have been functionally characterised rice. Heterologous expression Heavy metal‐associated Isoprenylated Plant Protein 9 (OsHIPP9), metallochaperone, altered yeast tolerance to cadmium (Cd) copper (Cu). We investigated the physiological role OsHIPP9 was primarily expressed root exodermis xylem region enlarged vascular bundles (EVB) at...
Aeromonas species are among the main pathogens causing rainbow trout infections. Silver nanoparticles (AgNPs) have a broad spectrum of antimicrobial properties and usually produced by various green-synthesis methods. However, application commercialized AgNPs has not fully been clarified. Thus, objective this study was to evaluate antibacterial activities (range sizes 10–12 nm) on two contrasting A. salmonicida strains (I-1 I-4), isolated from trout; mechanism, histopathological alterations...
This study aims to explore the impacts of ApoB-100/SORT1-mediated immune microenvironment during acute spinal cord injury (SCI), and investigate potential mechanism. CB57BL/6 mice underwent moderate thoracic contusion establish SCI animal model, received ApoB-100 lentivirus injection interfere level. Functional recovery was assessed using Basso, Beattie, Bresnahan (BBB) score footprint analysis. Transmission electron microscopy applied observe ultrastructure injured tissue. Hematoxylin-eosin...
Abstract The endoplasmic reticulum (ER) quality control system monitors protein homeostasis and relies on the activity of many molecular chaperones. Binding immunoglobulin (BiP) is a major ER luminal chaperone that involved in most functions organelle. BiP tightly regulated by nucleotide exchange factors (NEFs). However, information about NEFs plants limited. We obtained Fes1-like (OsFes1C) through isobaric tags for relative absolute quantitation-based proteomics analysis ER-stressed rice...
Peptides derived from food protein have the potential to become antihypertensive agents with relatively few negative side effects. Herein, multiple peptides, extracted transgenic rice seed, were administered intragastrically into spontaneously hypertensive rats (SHRs) different dosages, resulting in a significant decrease systolic blood pressure (SBP). Furthermore, for period of 5 weeks, daily intragastric administration flour also significantly reduced SBP SHRs but not Wistar Kyoto...
Maize, as a glycophyte, is hypersensitive to salinity, but the salt response mechanism of maize remains unclear. In this study, physiological, biochemical, and molecular responses two contrasting inbred lines, salt-tolerant QXH0121 salt-sensitive QXN233 were investigated in stress. Under stress, tolerant line exhibited good performance, while sensitive line, there negative effects on growth leaves roots. The most important finding was that could reshift Na+ from shoots into long roots,...
Abstract The nucleic acid transport properties of the systemic RNAi-defective (SID) 1 family make them attractive targets for developing RNA-based therapeutics and drugs. However, molecular basis double-stranded (ds) RNA recognition by SID1 remains elusive. Here, we report cryo-EM structures Caenorhabditis elegans (c) alone in complex with dsRNA, both at a resolution 2.2 Å. dimeric cSID1 interacts two dsRNA molecules simultaneously. is located interface between β-strand rich domain (BRD)1...
The Arabidopsis thaliana aluminum-activated malate transporter 9 (AtALMT9) functions as a vacuolar chloride channel that regulates the stomatal aperture. Here, we present cryoelectron microscopy (cryo-EM) structures of AtALMT9 in three distinct states. forms dimer, and pore is lined with four positively charged rings. apo-AtALMT9 state shows putative endogenous citrate obstructing pore, where two W120 constriction residues enclose gate radius approximately 1.8 Å, representing an open state....
The cryo-EM structure of the human Meckelin reveals a unique protein fold.
Human RBM25 (RNA-binding motif protein 25) is a novel splicing factor that contains PWI domain, newly identified RNA/DNA-binding and regulates Bcl-x pre-mRNA alternative splicing. The flanking basic region has been suggested to serve as co-operative partner of the domain in binding nucleic acids, but structure this unknown. In present paper, we report crystal its region. revealed comprise conserved four-helix bundle, forms two α-helices associates with helix H4 domain. These interactions...
PKCι/λ has been proposed to be crucial in the early expression of long-term potentiation (LTP). Here, we further investigate potential role learning and memory by generating conditional knockout mice specifically lacking hippocampal CA1 pyramidal cells. Surprisingly, cKO show normal LTP memory. Further close-up observation reveals compensation for PKMζ mice. This was not observed under basal conditions, but detected either after induction or learning-associated behavioral training....
Rice glutelins are initially synthesized as 57-kDa precursors at the endoplasmic reticulum (ER) and ultimately transported into protein storage vacuoles. However, sequence motifs that affect proglutelin folding, assembly, their export from ER remain poorly defined. In this study, we characterized a mutant with nine amino acids deleted in GluA2 protein, which resulted specific accumulation of GluA precursor. The constitute well-conserved (LVYIIQGRG) all residues motif necessary for GluA2....
<b><i>Objectives:</i></b> The aim of this investigation was to study the underlying mechanism an adenosine A<sub>1</sub> receptor agonist 2-chloro-N6 cyclopentyladenosine (CCPA) inhibiting cardiomyocyte hypertrophy induced by angiotensin II (AngII). <b><i>Methods:</i></b> Neonatal rat cardiomyocytes were treated with AngII generate a model. Cardiomyocyte cultures randomized into 5 groups: control; AngII; + cyclosporin A (CsA); CCPA,...
Enhanced beta oscillations within the cortico-basal ganglia-thalamic (CBT) network are correlated with motor deficits in Parkinson's disease (PD), whose generation has been associated recently amplified dynamics striatum. However, how distinct striatal cell subtypes interact to orchestrate remains largely unknown. Here, we show that optogenetic suppression of dopaminergic control over dorsal striatum (DS) elevates power local field potentials (LFPs) selectively at band (12-25 Hz),...
An alkalophilic methanogen T13 was isolated by anaerobic techniques of Hungate from cow muck in Hongyuan county,Sichuan,China enriched culture for at 15 ℃.Its optimum pH producing methane 8.5,and the temperature growth observed range 8~45 ℃.The visible particulate.Cells were aggregated and gram-positive.The colony pale yellow,mulberry-like fluoresced.T13 could grow using acetate,methanol or methylamine as sole carbon source,and it sensitive to chloramphenicol gentamicin.The NaCl...
Abstract Weissella ceti , one of Gram-positive lactic‐acid bacteria, as a newly emerging pathogen can cause the rainbow trout ( Oncorhynchus mykiss ) with Weissellosis. Until now, it is not easy to identify W. from other pathogens by symptoms fish infected especially at primary stage infection, and thus method identification was needed determine conveniently quickly this pathogen. In study, loop-mediated isothermal amplification (LAMP) detection has higher specificity sensitivity for . The...
Weissella ceti , a Gram‐positive lactic acid bacterium, has emerged as novel pathogen causing Weissellosis in rainbow trout ( Oncorhynchus mykiss ). Until now, it not been easy to distinguish W. from other pathogens by the symptoms of infected trout, especially at primary stage infection. Thus, convenient and rapid identification method is needed determine this pathogen. The current study demonstrated loop-mediated isothermal amplification (LAMP) superior specificity sensitivity for ....