A5083829536- Receptor Mechanisms and Signaling
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroscience and Neuropharmacology Research
- Zebrafish Biomedical Research Applications
- Erythrocyte Function and Pathophysiology
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Neuropeptides and Animal Physiology
- RNA regulation and disease
- Lipid Membrane Structure and Behavior
- Neuroscience of respiration and sleep
- Folate and B Vitamins Research
- Neurogenesis and neuroplasticity mechanisms
- Neurobiology and Insect Physiology Research
- Hearing Loss and Rehabilitation
- CRISPR and Genetic Engineering
- Cellular transport and secretion
- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Cannabis and Cannabinoid Research
- Tryptophan and brain disorders
- Nerve injury and regeneration
- interferon and immune responses
ShanghaiTech University
2017-2025
Shantou University
2023-2025
Fudan University
2025
South China University of Technology
2022-2023
China Academy of Space Technology
2023
Shanghai Ninth People's Hospital
2022
Shanghai Jiao Tong University
2022
Harvard University
2012-2019
Guangzhou Institutes of Biomedicine and Health
2019
Chinese Academy of Sciences
2019
Actin and spectrin play important roles in neurons, but their organization axons dendrites remains unclear. We used stochastic optical reconstruction microscopy to study the of actin, spectrin, associated proteins neurons. formed ringlike structures that wrapped around circumference were evenly spaced along axonal shafts with a periodicity ~180 190 nanometers. This periodic structure was not observed dendrites, which instead contained long actin filaments running dendritic shafts. Adducin,...
Imaging membranes in live cells with nanometer-scale resolution promises to reveal ultrastructural dynamics of organelles that are essential for cellular functions. In this work, we identified photoswitchable membrane probes and obtained super-resolution fluorescence images membranes. We demonstrated the photoswitching capabilities eight commonly used probes, each specific plasma membrane, mitochondria, endoplasmic recticulum (ER) or lysosomes. These small-molecule readily label high probe...
Actin, spectrin, and associated molecules form a periodic sub-membrane lattice structure in axons. How this membrane skeleton is developed why it preferentially forms axons are unknown. Here, we studied the developmental mechanism of structure. We found that emerged early during axon development propagated from proximal regions to distal ends Components initial segment were recruited late development. Formation was regulated by local concentration βII which higher than dendrites. Increasing...
Abstract Hearing loss is the most common sensory disorder. While gene therapy has emerged as a promising treatment of inherited diseases like hearing loss, it dependent on identification delivery vectors. Adeno-associated virus (AAV) vector-mediated been approved in US for treating rare eye disease but no safe and efficient vectors have identified that can target diverse types inner ear cells. Here, we identify an AAV variant, AAV-inner (AAV-ie), mouse ear. Our results show AAV-ie transduces...
Messenger RNA (mRNA) encoding base editors, along with single guide RNAs (sgRNAs), have emerged as a promising therapeutic approach for various disorders. However, there is still insufficient exploration in achieving targeted and efficient delivery of mRNA sgRNA to multiple organs while ensuring high biocompatibility stability vivo. To address this challenge, we synthesized library 108 poly(β-amino) esters (PBAEs) by incorporating 100% hydrophobic side chains end-caps varying amines. These...
Many animals are capable of changing gait with speed locomotion. The neural basis control and its dependence on not fully understood. Mice normally use a single “trotting” while running at all speeds, either over ground or treadmill. Transgenic mouse mutants in which the trotting is replaced by hopping also lack speed-dependent change gait. Here we describe transgenic model V2a interneurons have been ablated targeted expression diphtheria toxin A chain (DTA) under Chx10 gene promoter...
The V2a class of Chx10-expressing interneurons has been implicated in frequency-dependent control left–right phase during locomotion the mouse. We have used Chx10::CFP mouse line to further investigate properties and locomotion-related activity isolated neonatal spinal cord. can be divided into three classes, based on their tonic, phasic, or delayed-onset responses step depolarization. Electrical coupling is found only between neurons same helps synchronize neuronal within class. Serotonin...
Super-resolution fluorescence imaging reveals a previously unknown novel structure of spectrin in inner ear hair cells.
We explored the organization of spinal central pattern generator (CPG) for locomotion by analysing activity interneurons and motoneurons during spontaneous deletions occurring fictive in isolated neonatal mouse cord, following earlier work on locomotor cat. In most were non-resetting, with rhythmic resuming after an integer number cycles. Flexor extensor showed marked asymmetry: flexor accompanied sustained ipsilateral activity, whereas bursting was not perturbed deletions. Rhythmic one side...
Abstract Human adipose-derived stem cells (hADSCs) are increasingly presumed to be a prospective cell source for replacement therapy in various degenerative and/or traumatic diseases. The potential of trans-differentiating hADSCs into motor neuron indisputably provides an alternative way spinal cord injury (SCI) treatment. In the present study, stepwise and efficient hADSC trans-differentiation protocol with retinoic acid (RA), sonic hedgehog (SHH), neurotrophic factors were developed. With...
Rationale: As a hallmark of various heart diseases, cardiac fibrosis ultimately leads to end-stage failure. Anti-fibrosis is potential therapeutic strategy for Long noncoding RNAs (lncRNAs) have emerged as critical regulators diseases that promise serve targets. However, few lncRNAs been directly implicated in fibrosis. Methods: The lncRNA expression profiles were assessed by microarray fibrotic and remote ventricular tissues mice with myocardial infarction. mechanisms functional...
Abstract The cochlea consists of multiple types cells, including hair supporting cells and spiral ganglion neurons, is responsible for converting mechanical forces into electric signals that enable hearing. Genetic environmental factors can result in dysfunctions cochlear auditory systems. In recent years, gene therapy has emerged as a promising treatment animal deafness models. One major challenge the to effectively deliver genes specific cochleae. Here, we screened identified an AAV-ie...
Abstract Targeted delivery of mRNA with lipid nanoparticles (LNPs) holds great potential for treating pulmonary diseases. However, the lack rational design principles efficient lung‐homing lipids hinders prevalence therapeutics in this organ. Herein, combinatorial screening structure‐function analysis is applied to rationalize strategy nonpermanently charged lung‐targeted ionizable lipids. It discovered that carrying N‐methyl and secondary amine groups heads, three tails originated from...
The persistent sodium current (I(Na(P))) has been implicated in the regulation of synaptic integration, intrinsic membrane properties, and rhythm generation many types neurons. We characterized I(Na(P)) commissural interneurons (CINs) neonatal (postnatal days 0-3) mouse spinal cord; it is activated at subthreshold potentials, inactivates slowly, can be blocked by low concentrations riluzole. role locomotor pattern was examined applying riluzole during fictive locomotion induced NMDA,...
Axon growth requires long-range transport of organelles, but how these cargoes recruit their motors and traffic is regulated are not fully resolved. In this paper, we identify a new pathway based on the class III PI3-kinase (PIK3C3), ankyrin-B (AnkB), dynactin, which promotes fast axonal synaptic vesicles, mitochondria, endosomes, lysosomes. We show that dynactin associates with cargo through AnkB interactions both subunit p62 phosphatidylinositol 3-phosphate (PtdIns(3)P) lipids generated by...
Axon degeneration sculpts neuronal connectivity patterns during development and is an early hallmark of several adult-onset neurodegenerative disorders. Substantial progress has been made in identifying effector mechanisms driving axon fragmentation, but less known about the upstream signaling pathways that initiate this process. Here, we investigate behavior actin-spectrin-based Membrane-associated Periodic Skeleton (MPS), effects actin spectrin manipulations sensory degeneration. We show...
Mitochondria and peroxisomes are independent but functionally closely related organelles. A few proteins have been characterized as dual-organelle locating with distinct or similar roles on mitochondria peroxisomes. MARCH5 is a mitochondria-associated ubiquitin ligase best known for its regulatory role in quality control, fission, fusion. Here, we used proximity tagging system, PUP-IT, identified new interacting of MARCH5. Our data uncover that protein interacts several peroxisomal proteins....
Cell-based ex vivo gene therapy in solid organs, especially the liver, has proven technically challenging. Here, we report a feasible strategy for clinical application of hepatocyte therapy. We first generated high-quality autologous hepatocytes through large-scale expansion patient-derived hepatocytes. Moreover, proliferating hepatocytes, together with AAV2.7m8 variant identified screening, enabled CRISPR-Cas9-mediated targeted integration efficiently, achieving functional correction...
Commissural interneurons (CINs) send their axons across the midline to innervate contralateral targets and have been implicated in coordination of left-right limb movements during locomotion. In neonatal mouse spinal cord, we studied firing properties responses serotonin (5-HT) two classes CINs: those whose turn caudally after crossing (dCINs) bifurcate (adCINs). During NMDA 5-HT-induced locomotor-like activity, a majority lumbar (L2) dCINs fired rhythmically with ventral root-recorded motor...
Self-assembling supramolecular nanofibers, common in the natural world, are of fundamental interest and technical importance to both nanotechnology materials science. Despite important advances, synthetic nanofibers still lack structural functional diversity biological molecules, controlled assembly one type molecule into a variety fibrous structures with wide-ranging attributes remains challenging. Here, we harness low-complexity (LC) sequence domain fused sarcoma (FUS) protein, an...