A5111694935- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Virus-based gene therapy research
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Galectins and Cancer Biology
- Pneumonia and Respiratory Infections
- Immunotherapy and Immune Responses
- Streptococcal Infections and Treatments
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Antimicrobial Peptides and Activities
- Genomics and Phylogenetic Studies
- Chemical Synthesis and Analysis
- Extracellular vesicles in disease
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Bacterial Genetics and Biotechnology
- MicroRNA in disease regulation
Centre of Excellence in Molecular Biology
2014-2024
University of the Punjab
2014-2024
University of Agriculture Faisalabad
2024
University of Alberta
1987-2000
UDP-3-<i>O</i>-(<i>R</i>-3-hydroxymyristoyl)-<i>N</i>-acetylglucosamine deacetylase (LpxC) catalyzes the second step in biosynthesis of lipid A, a unique amphiphilic molecule found outer membranes virtually all Gram-negative bacteria. Since A is required for bacterial growth, inhibitors LpxC have potential utility as antibiotics. The enzymes biosynthesis, including LpxC, are encoded by single copy genes sequenced genomes. We now cloned, overexpressed, and purified from hyperthermophile...
Green algae, Chlamydomonas reinhardtii, with low cultivation cost, absence of endotoxins and insusceptibility to human pathogens is emerging as a potential system for the future production recombinant proteins. The recent development molecular tools enabling protein expression in algae chloroplast has provided new research advance opportunities developing low-cost therapeutic In present study, was evaluated an anti-cancerous protein, Interleukin 29 (IL29). IL29 gene cloned into vector...
Recombinant consensus interferon (CIFN) is a therapeutic protein with molecular weight of 19.5 kDa having broad spectrum antiviral activity. human CIFN (rhCIFN) has previously been expressed in Escherichia coli using isopropyl-β-d-thiogalactopyranoside (IPTG), non-metabolizable and expensive compound, as inducer. For economical commercial-scale recombinant production, it greatly needed to increase the product yield limited time frame reduce processing cost. To cost production rhCIFN E. coli,...
Interleukin-2 has emerged as a potent protein-based drug to treat various cancers, AIDS, and autoimmune diseases. Despite its immense requirement, the production procedures are inefficient meet demand. Therefore, efficient must be adopted improve protein yield decrease procedural loss. This study analyzed cytoplasmic periplasmic IL-2 expression for increased significant biological activity. The is focused on cloning into pET-SUMO pET-28a vector that expresses in soluble form inclusion...
The trisaccharide βDGlcNAc(1 → 2)αDMan(1 6)βDMan (2) and the tetrasaccharide 6)[αDMan(1 3)]βDMan (3) have been chemically synthesized as their 8-methoxycarbonyloctyl glycosides. Compounds 2 3, both partial structures of naturally occurring asparagine linked oligosaccharides, are designed to function selective substrates in N-acetylglucosaminytransferase assays.
Recombinant human consensus interferon (rh-cIFN) is an artificially engineered (IFN) developed by recombining and reordering the protein sequences that exist in standard IFN. This recombination resulted into a drug has potential to work better than natural, In this study, we described optimized conditions for high-level expression recovery of biologically active IFN from inclusion bodies (IBs). A synthetic gene coding 166 amino acids was cloned under T7 promoter. Escherichia coli strain...
Biological activity of human interleukin-6 (IL-6) is associated with a vast number diseases such as rheumatoid arthritis, sepsis, and severe inflammatory diseases. In this study, IL-6 cDNA was isolated from library that constructed mRNA derived placental tissues. Subsequently, the complete cloned expressed in BL21DE3 cells. The recombinant (rhIL-6) protein form an insoluble inclusion body. Inclusion bodies were solubilized under denaturing conditions purified by immobilized metal affinity...
Interleukin-6 a pleiotropic cytokine involved in wide range of biological activities. So the large-scale production biologically active recombinant human interleukin-6 is important for its structural and functional studies. Here, we report an optimized method shake flask fermentation simplified high-yield purification procedure interleukin-6. This expression not only involves optimization condition but also single step as well two-step denaturing one-step refolding process. approach replaces...
Pakistan has one of the highest burdens pneumococcal diseases in world, but unfortunately studies this demanding research area are limited region. Pneumococcal surface protein A (PspA) is next generation vaccine candidate as locates on Streptococcus pneumoniae surface. Its gene, pspA, might be encoded by all pneumococci, and proven immunogenicity. The molecular characterization PspA, serotype distribution antibiotic susceptibility important for regional diversity studies.In study, we...
Recent advancement in fermentation technologies resulted the increased yields of recombinant proteins biopharmaceutical and medicinal importance. Consequently, there is an important task to develop simple easily scalable methods that can facilitate production high-quality protein. Most recent reports described expression human IL-1 receptor antagonist (rhIL-1Ra) Escherichia coli using isopropyl-β-d-thiogalacto pyranoside (IPTG), a nonmetabolizable expensive compound, as inducer. In this...
In certain low-income nations, the hepatitis Delta virus and B (HBV) pose a serious medical burden, where prevalence of surface antigen (HBsAg) is greater than 8%. Especially in rural places, irregular diagnostic exams are main restriction reason for underestimation. Utilizing serum samples from Pakistani isolate, an internal ELISA quick identification anti-HDV was created, effectiveness test compared to commercial kit. HDV-positive were collected, highly antigenic domain HDAg derived them....
Therapeutic proteins need to be produced in large quantities as they continuously gain higher shares pharmaceutical market. Human beta interferon (hIFN-β) is used against different diseases due its antiviral, antiproliferative and immunomodulatory nature. Considering clinical applications, development of a simple, cost-effective easily scalable production system produce rhIFN-β-1b protein high yield quality needed. Attempts develop improved methods are ongoing. Majority previous reports...
Folate, an essential nutrient crucial for DNA synthesis, cell division, and fetal neural tubedevelopment, remains a global health concern due to deficiencies in certain populations. Toaddress this issue, we investigated the synergistic interaction between Spirulina, nutrient-rich microalga, two strains of folic acid-producing bacteria, Bacillus subtilis-1 subtilis-2, enhance folate production. Spirulinahas high nutritional content, combined with production capabilities selected offered...
Abstract Scientists have implemented protein‐PEGylation technology for boosting‐up the pharmacokinetics and stability of recombinant therapeutic proteins. In present study, (a) matrix‐assisted PEGylation was compared with solution‐phase (b) performed different ion exchange resins impact chromatography medium on yield purity PEGylated product. DEAE Sepharose CL 6B, Fracto gel, Macro cap Q were column purification cIFN. A MSC‐PEG 12.0 KDa selected. cIFN bound to medium, PEG solution passed...
Previously, we have reported cloning of human epidermal growth factor gene from Huh-7 cells and its extracellular expression in Pichia pastoris. The presented work is a detailed report regarding molecular characterization cells-derived hEGF expressed pastoris with special reference to glycosylation profiling bioactivity studies. Densitometric scanning SDS-PAGE separated proteins recombinant strain indicated that about 84% the were glycosylated. Size exclusion chromatography using Superdex 75...