A5040864652- Infant Nutrition and Health
- Neonatal Respiratory Health Research
- Viral gastroenteritis research and epidemiology
- Clinical Nutrition and Gastroenterology
- Digestive system and related health
- Inflammatory Bowel Disease
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Neonatal and Maternal Infections
- Vitamin C and Antioxidants Research
- Antimicrobial Resistance in Staphylococcus
- Systemic Lupus Erythematosus Research
- Celiac Disease Research and Management
- Streptococcal Infections and Treatments
- Long-Term Effects of COVID-19
- Sepsis Diagnosis and Treatment
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Infant Health and Development
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Probiotics and Fermented Foods
Seattle Children's Hospital
2023-2024
University of Washington
2023-2024
MedStar Georgetown University Hospital
2020-2022
Georgetown University
2020-2022
Georgetown University Medical Center
2022
University of Illinois Urbana-Champaign
2012-2018
Background: Secretory immunoglobulin A (sIgA) plays an important role in antiviral protective immunity. Although salivary testing has been used for many viral infections, including severe acute respiratory syndrome (SARS) and Middle East Respiratory Syndrome (MERS), its use not yet well established with the SARS coronavirus 2 (SARS-CoV-2). Quantification of IgA IgG antibodies can elucidate mucosal systemic immune responses after natural infection or vaccination. Here, we report development...
Bovine lactoferrin (bLf) reduces
Abstract Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that may involve any organ in the body. Inflammation of bowel wall as presenting symptom SLE uncommon and can lead to delays diagnosis treatment. Here, we discuss case an adolescent male who presented with weight loss, intermittent fevers, abdominal pain, vomiting, diarrhea. Initially, inflammatory (IBD) was suspected, but endoscopic evaluation did not support this diagnosis. A computed tomography scan abdomen...
To investigate serum immune/inflammatory profiles in preterm very low birthweight (BW) infants enrolled a randomized trial of daily supplementation with multi-strain probiotic. A central hypothesis is probiotics will promote T cell homeostasis via regulatory (Treg) response and suppress inflammatory Th1/Th17 cytokine to intestinal microbiota diversity, reduce inflammation, support barrier function. After parental informed consent, admitted neonatal ICU are into 2 groups (+/- probiotics;...
Staphylococcus aureus ( S. ) is a primary cause of death due to sepsis in neonatal intensive care units. Fatal characterized by systemic inflammatory response syndrome (SIRS), leading inflammation and organ dysfunction. Herein, clinically‐relevant piglet model SIRS secondary infection was developed. Colostrum‐deprived pigs had umbilical catheters placed within 12h birth were fed sow's milk replacer formula. On d7, piglets either non‐infected (n=2) or injected IV with 1ml/kg BW (strain S54F9)...
Bovine lactoferrin (bLf) reduces Staphylococcus aureus infection in premature infants and promotes the growth of Bifidobacterium infantis, a predominant infant gut species. We hypothesized bLf combination with B. infantis would reduce severity systemic S. infection. To determine effects oral administration on course Colostrum-deprived piglets consumed formulas containing 4g/l whey (CON) or (LF). Half were gavaged (109 CFU/d) resulting two additional groups (BI COMB). On d7, intravenously...
Infections in infants caused by antibiotic resistant S. aureus have limited treatment options. In a clinical trial, premature fed formula with bovine lactoferrin (bLf) had decreased blood‐borne staphylococcal infections, but the mechanism was not known. Additionally, Lf promotes growth of B. infantis , predominant species breast‐fed infant intestine. Herein, effect bLf alone or combination on course infection assessed. Colostrum‐deprived pigs umbilical catheters placed at birth and were 4g/L...
The method most commonly used to label newly divided cells is a thymidine analogue, 5‐bromo‐2′‐deoxyuridine (BrdU). BrdU detection requires extensive DNA denaturation. However, an alternative 5‐ethynyl‐2′‐deoxyuridine (EdU) can be used. EdU that has been incorporated into formed tagged by covalently bound fluorescent azide molecule allowing for highly specific recognition without disruption. We have optimized technology in vivo use pigs using confocal microscopy as novel approach quantify...
Human milk contains factors, such as lactoferrin(LF), that stimulate gastrointestinal growth and maturation. Herein, proliferative properties of bovine LF(bLF) were explored through 5‐ethynyl‐2′‐deoxyuridine(EdU) incorporation in vivo. Colostrum‐deprived pigs fed formula containing 0.4(CON), 1(LF1) or 3.6(LF3) g bLF/L. On d7 14, intraperitoneally injected with 10mM EdU(1ml/kg body weight) 2h prior to euthanasia. Proliferation was assessed ex vivo formalin‐fixed jejunal sections using...