A5044495612- Spectroscopy Techniques in Biomedical and Chemical Research
- Neurogenetic and Muscular Disorders Research
- Genetic Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Total Knee Arthroplasty Outcomes
- Digital Holography and Microscopy
- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Ubiquitin and proteasome pathways
- Peripheral Neuropathies and Disorders
- Optical Coherence Tomography Applications
- Muscle Physiology and Disorders
- Advanced Fluorescence Microscopy Techniques
- Knee injuries and reconstruction techniques
- Mitochondrial Function and Pathology
- Spectroscopy and Chemometric Analyses
- Muscle activation and electromyography studies
- Photoacoustic and Ultrasonic Imaging
- Peripheral Nerve Disorders
- Hereditary Neurological Disorders
- Gait Recognition and Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Photoreceptor and optogenetics research
- Diabetic Foot Ulcer Assessment and Management
- Genetics and Neurodevelopmental Disorders
The University of Tokyo
2012-2024
Oita University
2018-2024
Hamamatsu University School of Medicine
2022-2024
Toranomon Hospital
2016-2017
Center for Global Health
2017
National Center for Global Health and Medicine
2017
National Defense Medical College
2012-2017
Mita Hospital
2017
Yokohama City University
2017
Yokohama City University Medical Center
2017
Amyloid β protein (Aβ), a pathogenic molecule associated with Alzheimer's disease, is produced by γ-secretase, which cleaves the β-carboxyl terminal fragment (βCTF) of β-amyloid precursor in middle its transmembrane domain. How cleavage proceeds within membrane has long been enigmatic. We hypothesized previously that βCTF cleaved first at membrane–cytoplasm boundary, producing two Aβs, Aβ 48 and 49 , are processed further releasing three residues each step to produce 42 40 respectively. To...
Label-free optical imaging is valuable in biology and medicine because of its non-destructive nature. Quantitative phase (QPI) molecular vibrational (MVI) are the two most successful label-free methods, providing morphological biochemical information, respectively. These techniques have enabled numerous applications as they matured over past few decades; however, their contrasts inherently complementary difficult to integrate due reliance on different light–matter interactions. Here we...
Gait movies recorded in daily clinical practice are usually not filmed with specific devices, which prevents neurologists benefitting from leveraging gait analysis technologies. Here we propose a novel unsupervised approach to quantifying features and extract cadence normal parkinsonian home video camera by applying OpenPose, deep learning-based 2D-pose estimator that can obtain joint coordinates pictures or videos monocular camera.Our proposed method consisted of two distinct phases:...
Huntington disease and its related autosomal-dominant polyglutamine (pQ) neurodegenerative diseases are characterized by intraneuronal accumulation of protein aggregates. Studies on aggregates have revealed the importance ubiquitin-proteasome system as front line quality control (PQC) machinery against aberrant proteins. Recently, we shown that autophagy-lysosomal is also involved in cytoplasmic aggregate degradation, but nucleus lacked this activity. Consequently, relies entirely for PQC....
Ubiquitin-like 3 (UBL3) acts as a post-translational modification (PTM) factor and regulates protein sorting into small extracellular vesicles (sEVs). sEVs have been reported vectors for the pathology propagation of neurodegenerative diseases, such α-synucleinopathies. Alpha-synuclein (α-syn) has widely studied its involvement in However, it is still unknown whether UBL3 interacts with α-syn, influenced by drugs or compounds. In this study, we investigated interaction between any ensuing...
Research Article21 February 2012Open Access Altered γ-secretase activity in mild cognitive impairment and Alzheimer's disease Nobuto Kakuda Immuno-Biological Laboratories Co., Fujioka, Japan Faculty of Life Medical Sciences, Department Neuropathology, Doshisha University, Kyoto, New Energy Industrial Technology Development Organization (NEDO), Kanagawa, Search for more papers by this author Mikio Shoji Neurology, Institute Brain Science, Hirosaki University Graduate School Medicine,...
Abstract An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial medical settings. A standard benchtop platform, equipped with e.g., bright-field phase-contrast modes, of importance convenience for various users because the wide-field label-free properties allow morphological imaging without need specific sample preparation. However, these microscopes never have capability acquiring molecular contrast a...
<h3>Objective:</h3> To determine the clinical features of myositis patients with histopathologic finding CD8-positive T cells invading non-necrotic muscle fibers expressing major histocompatibility complex class 1 (CD8-MHC-1 complex), which is shared by polymyositis (PM) and inclusion body (IBM), in relation to p62 immunostaining pattern fibers. <h3>Methods:</h3> All 93 CD8-MHC-1 who were referred our hospital from 1993 2015 classified on basis European Neuromuscular Center (ENMC) diagnostic...
To report the ocular characteristics of neuronal intranuclear inclusion disease (NIID)-related retinopathy with expansion CGG repeats in NOTCH2NLC gene.Seven patients from six families (aged 66-81 years) diagnosed adult-onset NIID were studied. Ophthalmologic examinations, including best-corrected visual acuity (BCVA), Goldmann perimetry, fundus photography, autofluorescence (FAF) imaging, optical coherence tomography (OCT), and full-field electroretinography (ERGs), performed. The gene was...
Endocannabinoid 2-arachidonoylglycerol (2-AG) has been implicated in habituation to stress, and its augmentation reduces stress-induced anxiety-like behavior. Chronic restraint stress (CRS) changes the 2-AG levels some gross brain areas, such as forebrain. However, detailed spatial distribution of by CRS processing-related anatomical structures anterior cingulate cortex (ACC), caudate putamen (CP), nucleus accumbens (NAc), piriform (PIR) are still unclear. In this study, mice were restrained...
Abstract Introduction: A reliable electrophysiological marker for clinical trials is increasingly needed in spinal and bulbar muscular atrophy (SBMA). We previously developed a quantitative analysis method surface electromyography (SEMG), the clustering index (CI) method. Our purpose was to test utility of CI evaluating lower motor neuron involvement SBMA patients. Methods: Subjects included 29 patients 27 healthy controls. The recording electrode placed over abductor digiti minimi (ADM)...
Abstract Ubiquitin-like 3 (UBL3) acts as a post-translational modification (PTM) factor and regulates protein sorting into small extracellular vesicles (sEVs). sEVs have been reported vectors for the pathology propagation of neurodegenerative diseases, such α-synucleinopathies. Alpha-synuclein (α-syn) has widely studied its involvement in However, it is still unknown whether UBL3 interacts with α-syn, influenced by drugs or compounds. In this study, we investigated interaction between any...
Expansion of polyglutamine (pQ) chain by expanded CAG repeat causes dominantly inherited neurodegeneration such as Huntington disease, dentatorubral-pallidoluysian atrophy (DRPLA), and numbers other spinocerebellar ataxias. Expanded pQ disrupts the stability pQ-harboring protein increases its susceptibility to aggregation. Aggregated is recognized ubiquitin proteasome system, enzyme ligase covalently attaches ubiquitin, which serves a degradation signal proteasome. However, accumulation...
Abstract Objective Gait movies recorded in daily clinical practice are usually not filmed with specific devices, which prevents neurologists benefitting from leveraging gait analysis technologies. Here we propose a novel unsupervised approach to quantifying features and extract cadence normal parkinsonian home video camera by applying OpenPose, deep learning–based 2D-pose estimator that can obtain joint coordinates pictures or videos monocular camera. Methods Our proposed method consisted of...
To delineate molecular and clinical characteristics of 3 families with PRNP P105L mutation, a variant Gerstmann-Sträussler-Scheinker syndrome whose main motor symptoms were parkinsonism and/or involuntary movements.The causative mutation was first determined in the affected patients family 1 using whole-exome sequencing, then mutational analysis extended to 2 3. The features these summarized. Haplotype performed high-density single nucleotide polymorphism array.The sequencing revealed that...
Ubiquitin-like 3 (UBL3) is a membrane-anchored protein that plays crucial role in sorting proteins into small extracellular vesicles. Aggregations of alpha-synuclein (α-syn) are associated with the pathology neurodegenerative diseases such as Parkinson's disease. Recently, interaction between UBL3 and α-syn was discovered, potential implications clearing excess from neurons its disease spread. However, regulator can mediate remains unclear. In this study, using split gaussian luciferase...
Halogenated volatile anesthetics are frequently used for inhaled anesthesia in clinical practice. No appropriate biological method has been available visualizing their localization action. Therefore, despite frequent use, the mechanism of action these drugs not fully investigated. We measured coherent anti-Stokes Raman scattering (CARS) spectra sevoflurane and isoflurane, two most representative anesthetics, determined low-frequency vibrational modes without nonresonant background...
The spectral resolution of broadband Fourier-transform coherent anti-Stokes Raman spectroscopy is limited by the maximum optical path length difference that can be scanned within a short time in an interferometer. However, alternatives to exist which bypass this limitation with certain assumptions. We apply one such approach using interferometers delay line (low Fourier resolution) and large (high resolution). With method, we demonstrate closely spaced vibrational bands possible...
Abstract Ubiquitin-like 3 (UBL3) is a membrane-anchored protein that has been discovered to function as post-translational modifier, helping sort proteins into small extracellular vesicles (sEVs). Aggregations of alpha-synuclein (α-syn) are associated with the pathology neurodegenerative diseases such Parkinson’s disease (PD). The aggregation and toxicity α-syn can be influenced by its interactions specific proteins. Recently, between UBL3 was uncovered. It believed play role in eliminating...