A5101958683- Adsorption and biosorption for pollutant removal
- Enzyme Catalysis and Immobilization
- Catalytic C–H Functionalization Methods
- Asymmetric Hydrogenation and Catalysis
- Analytical chemistry methods development
- Molecular Sensors and Ion Detection
- Chemical Synthesis and Reactions
- Synthesis and Catalytic Reactions
- Microbial Metabolic Engineering and Bioproduction
- Mesoporous Materials and Catalysis
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Asymmetric Synthesis and Catalysis
- Advanced biosensing and bioanalysis techniques
- Luminescence and Fluorescent Materials
- Nanomaterials for catalytic reactions
- Water Quality Monitoring and Analysis
- Extraction and Separation Processes
- Pancreatic function and diabetes
- Chemical Synthesis and Characterization
- Natural Compounds in Disease Treatment
- Cyclopropane Reaction Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Click Chemistry and Applications
- Microbial Natural Products and Biosynthesis
Beijing Forestry University
2025
Tsinghua University
2008-2024
Beijing Tian Tan Hospital
2024
Capital Medical University
2024
Zhejiang Gongshang University
2015-2024
Center for Life Sciences
2021-2022
King Center
2021-2022
Beijing Advanced Sciences and Innovation Center
2021-2022
Ocean University of China
2020-2021
Shanghai Normal University
2019-2020
KRAS mutation occurs in nearly 30% of human cancers, yet the most prevalent and oncogenic KRAS(G12D) variant still lacks inhibitors. Herein, we designed a series potent inhibitors that can form salt bridge with KRAS's Asp12 residue. Our ITC results show these have similar binding affinity both GDP-bound GTP-bound KRAS(G12D), our crystallographic studies reveal structural basis inhibitor binding-induced switch-II pocket experimentally confirming formation between piperazine moiety residue...
Deoxygenation of phosphine oxides is great significance to synthesis phosphorus ligands and relevant catalysts, as well the sustainability chemistry. However, thermodynamic inertness P═O bonds poses a severe challenge their reduction. Previous approaches in this regard rely primarily on type bond activation with either Lewis/Brønsted acids or stoichiometric halogenating reagents under harsh conditions. Here, we wish report novel catalytic strategy for facile efficient deoxygenation via...
The first example of copper-catalyzed enantioselective dearomative azidation β-naphthols using a readily available N3-transfer reagent is reported. A series 2-hydroxy-1-naphthamides bearing complex N-substituent were converted to the corresponding products in high yields with up 96% ee, and chiral 1-azido-2-hydroxy-1-naphthoates obtained 90% ee under mild reaction conditions. azides could be further transformed into 1,2,3-triazoles smoothly via "click" reaction.
Controlling product selectivity in successive reactions of the same type is challenging owing to comparable thermodynamic and kinetic properties involved. Here, synergistic interaction two phosphoryl groups bisphosphine dioxides (BPDOs) with a bromo-phosphonium cation was studied experimentally provide practical tool for substrate–catalyst recognition. As eventual result, we have developed phosphonium-catalyzed monoreduction chiral BPDOs access an array synthetically useful monoxides (BPMOs)...
A penicillin acylase variant was designed to catalyze the enzymatic synthesis of β-lactam antibiotic cephradine under industrial conditions.
Abstract Obesity is a global issue that warrants the identification of more effective therapeutic targets and better understanding pivotal molecular pathogenesis. Annexin A1 (ANXA1) known to inhibit phospholipase A2, exhibiting anti-inflammatory activity. However, specific effects ANXA1 in obesity underlying mechanisms action remain unclear. Our study reveals levels are elevated adipose tissue individuals with obesity. Whole-body or adipocyte-specific deletion aggravates metabolic disorders....
The shoot apex of Populus alba primarily comprises the apical meristem, axillary leaf primordium, and young leaves, all which exhibit high division potential. single-cell RNA sequencing buds P. can provide deeper insights into processes cell proliferation differentiation, including key genes signaling pathways that regulate these processes. Scanning electron microscopy was used to examine structure apex, followed by analysis. A total 29,011 cells were obtained from two biological replicates....
The poor predictive accuracy of current computational enzyme design methods has led to low success rates producing highly active variants that target non-natural substrates. In this report, a quantitative assessment approach based on molecular dynamics (MD) simulations was developed eliminate false-positive designs at the stage. Taking cephradine hydrolase as an example, apparent Michaelis binding constant (K m) and catalytic efficiency (k cat/K designed were correlated with free energies...
We have developed a streamlined proteomic sample preparation protocol termed Accelerated Barocycler Lysis and Extraction (ABLE) that substantially reduces the time cost of tissue processing. ABLE is based on pressure cycling technology (PCT) for rapid solubilization reliable, controlled proteolytic digestion. Here, previously reported PCT was optimized using 1–4 mg biopsy punches from rat kidney. The denaturant urea substituted with combination sodium deoxycholate (SDC) N-propanol. produced...
Active site preorganization helps native enzymes electrostatically stabilize the transition state better than ground for their primary substrates and achieve significant rate enhancement. In this report, we hypothesize that a complex active model modeling should help to create preorganized design afford higher starting activities towards target reactions. Our matching algorithm ProdaMatch was improved by invoking effective pruning strategies sites ten scaffolds in benchmark test set were...
A new protein scaffold was identified and redesigned to catalyze the synthesis of β-lactam antibiotic cephradine.
C═C bond breaking to access the C═N remains an underdeveloped area. A new protocol for cleavage of alkenes under nonoxidative conditions produce imines via iron-catalyzed nitrene transfer reaction 4-hydroxystilbenes with aryl azides is reported. The success various sequential one-pot reactions reveals that good compatibility this method makes it very attractive synthetic applications. On basis experimental observations, a plausible mechanism also proposed.
An efficient rhodium-catalyzed redox-neutral annulations of N-phenoxyacetamides and ynones via successive double C–H bond activations has been developed. A series novel complex indenols bearing a benzofuran unit were generated with moderate to excellent regioselecetivities under mild conditions. Adding N-ethylcyclohexanamine (CyNHEt) could restrict the formation mono activation byproduct, which is not intermediate reaction demonstrated mechanistic investigations.
The Vilsmeier-Haack reaction is a powerful tool to introduce formyl groups into electron-rich arenes, but its wide application significantly restricted by stoichiometric employment of caustic POCl3. Herein, we reported catalytic version the enabled P(III)/P(V)═O cycle. This provides facile and efficient route for direct construction C1-deuterated indol-3-carboxaldehyde under mild conditions with DMF-d7 as deuterium source. products feature remarkably higher deuteration level (>99%) than...
Carbon dots (CDs) with blue fluorescence were synthesized using indole-3-butyric acid and l-tryptophan a one-step hydrothermal method. The CDs further employed as fluorescent sensor high selectivity for colorimetric ratiometric detection of tetracycline (TC) in water. limit (LOD) was found to be 0.33 μM TC R2 = 0.98387. Besides, the could applied practical water samples showed good recovery.
Copper-catalyzed nitrene transfer for amination/cyclization of tryptophols and tryptamines to generate the corresponding indole alkaloids in good excellent yields was successfully developed.