A5056977170- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Single-cell and spatial transcriptomics
- Renal Transplantation Outcomes and Treatments
- RNA Research and Splicing
- Mathematical Biology Tumor Growth
- Renal Diseases and Glomerulopathies
- Genomics and Phylogenetic Studies
- Evolution and Genetic Dynamics
- Glioma Diagnosis and Treatment
- Genetics and Neurodevelopmental Disorders
- Autism Spectrum Disorder Research
- Scientific Computing and Data Management
- Youth Development and Social Support
- Gene Regulatory Network Analysis
- Mycobacterium research and diagnosis
- Complement system in diseases
- Career Development and Diversity
- RNA regulation and disease
- Chromosomal and Genetic Variations
- Teaching and Learning Programming
- Gene expression and cancer classification
- Cancer-related gene regulation
Institute for Systems Biology
2024
InSysBio (Russia)
2024
Albert Einstein College of Medicine
2010-2015
Genomics (United Kingdom)
2013
Yeshiva University
2012
Buck Institute for Research on Aging
2006-2008
The University of Texas Health Science Center at San Antonio
2004
Differences in DNA repair capacity have been hypothesized to underlie the great range of maximum lifespans among mammals. However, measurements individual activities cells and animals not substantiated such a relationship because utilization pathways animals--depending on habitats, anatomical characteristics, life styles--varies greatly between mammalian species. Recent advances high-throughput genomics, combination with increased knowledge genetic involved genome maintenance, now enable...
DNA mutational events are increasingly being identified in autism spectrum disorder (ASD), but the potential additional role of dysregulation epigenome pathogenesis condition remains unclear. The is interest as a possible mediator environmental effects during development, encoding cellular memory reflected by altered function progeny cells. Advanced maternal age (AMA) associated with an increased risk having child ASD for reasons that not understood. To explore whether AMA involves covert...
Using a transgenic mouse model harboring mutation reporter gene that can be efficiently recovered from genomic DNA, we previously demonstrated mutations accumulate in aging mice tissue-specific manner. Applying recently developed, similar reporter-based assay Drosophila melanogaster, now show the frequency at lacZ locus somatic tissue of flies is about three times as high tissues, with much higher fraction large genome rearrangements. Similar to mice, fly also function age, but they do so...
Summary Background This study investigated the mechanisms involved in development of donor-specific antibody (DSA) and/or C4d-negative transplant glomerulopathy (TGP) by allograft gene expression profiles using microarrays. Design, Setting, Participants, & Measurements cohort was conducted kidney recipients. Patients were eligible for inclusion if they required a clinically indicated biopsy at any time point after their transplant. They then classified according to histopathology...
Abstract Consequential STEM experiences in informal settings can address issues of equity by fully engaging historically marginalized high school students complex socio-scientific issues. However, inclusive and effective programs are demand, there is little research on what specific aspects, context, timeframes most important when scaling these experiences. Using a mixed method approach, this study demonstrates that make significant gains, the short long term, through in-person remote...
Poor prognosis and drug resistance in glioblastoma (GBM) can result from cellular heterogeneity treatment-induced shifts phenotypic states of tumor cells, including dedifferentiation into glioma stem-like cells (GSCs). This rare tumorigenic cell subpopulation resists temozolomide, undergoes proneural-to-mesenchymal transition (PMT) to evade therapy, drives recurrence. Through inference transcriptional regulatory networks (TRNs) patient-derived GSCs (PD-GSCs) at single-cell resolution, we...
Infection with Plasmodium falciparum can lead to a range of severe minimal symptoms, occasionally resulting in death young children or nonimmune adults. In areas high transmission, older and adults generally suffer only mild asymptomatic malaria infections rarely develop disease. The immune features underlying this apparent immunity disease remain elusive. To gain insight into host responses associated malaria, we conducted longitudinal study five who first presented and, 1 month later,...
Recently, great progress has been made in single cell genomics and transcriptomics. Here, we present an integrative method, termed single-cell transcriptogenomics (SCTG), which whole exome sequencing RNA-seq is performed concurrently on cells. This methodology enables one to track germline somatic variants directly from the genome transcriptome individual Mouse embryonic fibroblasts were treated with powerful mutagen ethylnitrosourea (ENU) subjected SCTG. Interestingly, while found be...
ABSTRACT Mycobacterium haemophilum is an emerging pathogen associated with a variety of clinical syndromes, most commonly skin infections in immunocompromised individuals. M. exhibits unique requirement for iron supplementation to support its growth culture, but the basis this property and how it may shape pathogenesis unclear. Using combination Illumina, PacBio, Sanger sequencing, complete genome sequence was determined. Guided by sequence, experiments were performed define requirements ....
Abstract Background The BLM DNA helicase plays a vital role in maintaining genome stability. Mutations cause Bloom syndrome, rare disorder associated with cancer predisposition and premature aging. Humans mice blm mutations have increased frequencies of spontaneous mutagenesis, but the molecular basis this increase is not well understood. In addition, effect aging on mutagenesis mutants has been characterized. To address this, we used lacZ reporter system wild-type several mutant strains...
Affymetrix Human Gene 1.0-ST arrays were used to assess the gene expression profiles of kidney transplant patients who presented with donor-specific antibodies (DSAs) but showed normal biopsy histopathology and did not develop antibody-mediated rejection (AMR). Biopsy whole-blood for these DSA-positive, AMR-negative (DSA +/AMR-) compared both AMR-positive +/AMR +) as well DSA-negative -) controls. While individual changes across sample groups relatively subtle, gene-set enrichment analysis...
DNA mutational events are increasingly being identified in autism spectrum disorder (ASD), but the potential additional role of dysregulation epigenome pathogenesis condition remains unclear. The is interest as a possible mediator environmental effects during development, encoding cellular memory reflected by altered function progeny cells. Advanced maternal age (AMA) associated with an increased risk having child ASD for reasons that not understood. To explore whether AMA involves covert...
Systematic, high-throughput studies of mouse phenotypes have been hampered by the inability to analyze individual animal data from a multitude sources in an integrated manner. Studies generally make comparisons at level genotype or treatment thereby excluding associations that may be subtle involve compound phenotypes. Additionally, lack integrated, standardized ontologies and methodologies for exchange has inhibited scientific collaboration discovery. Here we introduce Mouse Phenotype...
ABSTRACT Poor prognosis and drug resistance in glioblastoma (GBM) can result from cellular heterogeneity treatment-induced shifts phenotypic states of tumor cells, including dedifferentiation into glioma stem-like cells (GSCs). This rare tumorigenic cell subpopulation resists temozolomide, undergoes proneural-to-mesenchymal transition (PMT) to evade therapy, drives recurrence. Through inference transcriptional regulatory networks (TRNs) patient-derived GSCs (PD-GSCs) at single-cell...
The use of sequencing technologies has revolutionized the field genomics, allowing us to study structural and functional variations within genome base pair level. These can also be used probe associated epigenome, where DNA-binding proteins alter integrity genome, restricting or enabling localized gene expression in a heritable fashion. By using assays that identify binding location these proteins, so called 'epigenetic marks' discover correlate molecular functions phenotypes being studied....