A5040370543- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Forensic Toxicology and Drug Analysis
- Analytical chemistry methods development
- Genetic Syndromes and Imprinting
- Alcohol Consumption and Health Effects
- Birth, Development, and Health
- Mass Spectrometry Techniques and Applications
- CRISPR and Genetic Engineering
- Chemical Synthesis and Reactions
- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Renal and related cancers
- Stress Responses and Cortisol
- Analytical Chemistry and Chromatography
- Genetics and Neurodevelopmental Disorders
- Cancer Genomics and Diagnostics
- Pesticide Residue Analysis and Safety
- Genomics and Chromatin Dynamics
- Molecular spectroscopy and chirality
- Asymmetric Synthesis and Catalysis
- Cannabis and Cannabinoid Research
- Synthetic Organic Chemistry Methods
- Pregnancy and preeclampsia studies
Hamamatsu University School of Medicine
2016-2025
Albert Einstein College of Medicine
2015-2024
Texas A&M University
2023-2024
Yokohama City Seibu Hospital
2024
St. Marianna University School of Medicine
2024
University of Utah
2024
Kanagawa Children's Medical Center
2024
Yeshiva University
2009-2023
Mitchell Institute
2023
Teikyo University
2020
The distribution of cytosine methylation in 6.2 Mb the mouse genome was tested using cohybridization genomic representations from a methylation-sensitive restriction enzyme and its methylation-insensitive isoschizomer. This assay, termed HELP ( H paII tiny fragment E nrichment by L igation-mediated P CR), allows both intragenomic profiling intergenomic comparisons methylation. profile shows most to be contiguous methylated sequence with occasional clusters hypomethylated loci, usually but...
DNA methylation and the Polycomb repression system are epigenetic mechanisms that play important roles in maintaining transcriptional repression. Recent evidence suggests can attenuate binding of protein components to chromatin thus plays a role determining their genomic targeting. However, whether this is context regulation unclear. By genome-wide mapping Repressive Complex 2-signature histone mark, H3K27me3, severely hypomethylated mouse somatic cells, we show hypomethylation leads...
One in eleven people is affected by chronic kidney disease, a condition characterized fibrosis and progressive loss of function. Epidemiological studies indicate that adverse intrauterine postnatal environments have long-lasting role disease development. Epigenetic information represents plausible carrier for mediating this programming effect. Here we demonstrate genome-wide cytosine methylation patterns healthy tubule samples obtained from patients show significant differences.We identify...
Deep penetration by substrates through the size-restricted channels of an apo-ferritin cage results in size-selective olefin hydrogenation at Pd nanocluster core (see picture). The encapsulated zero-valent cluster is synthesized situ chemical reduction PdII ions cage.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTrialkylsilyl triflates. 5. A stereoselective aldol-type condensation of enol silyl ethers and acetals catalyzed by trimethylsilyl trifluoromethanesulfonateS. Murata, M. Suzuki, R. NoyoriCite this: J. Am. Chem. Soc. 1980, 102, 9, 3248–3249Publication Date (Print):April 1, 1980Publication History Published online1 May 2002Published inissue 1 April 1980https://doi.org/10.1021/ja00529a062RIGHTS & PERMISSIONSArticle Views1631Altmetric-Citations249LEARN...
Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive their scientific medical utilities might prove limited. To evaluate characteristics of NT-ESCs, we established more than 150 NT-ESC lines adult somatic several mouse strains. Here, show that these were able...
Many genome-wide assays involve the generation of a subset (or representation) genome following restriction enzyme digestion. The use enzymes sensitive to cytosine methylation allows high-throughput analysis this epigenetic regulatory process. We show that dual-adapter approach us generate genomic representations includes fragments <200 bp in size, previously not possible when using standard single adapter. By expanding representation smaller HpaII or MspI, we increase by these isoschizomers...
Even though pancreatic ductal adenocarcinoma (PDAC) is associated with fibrotic stroma, the molecular pathways regulating formation of cancer fibroblasts (CAFs) are not well elucidated. An epigenomic analysis patient-derived and de-novo generated CAFs demonstrated widespread loss cytosine methylation that was overexpression various inflammatory transcripts including CXCR4. Co-culture neoplastic cells led to increased invasiveness abrogated by inhibition Metabolite tracing revealed lactate...
Although a combination of genomic and epigenetic alterations are implicated in the multistep transformation normal squamous esophageal epithelium to Barrett esophagus, dysplasia, adenocarcinoma, combinatorial effect these changes is unknown. By integrating genome-wide DNA methylation, copy number, transcriptomic datasets obtained from endoscopic biopsies neoplastic progression within same individual, we uniquely able define molecular events associated esophagus. We find that previously...
While DNA methylation plays a role in T-helper (Th) cell maturation, its potential dysregulation the non-atopic Th1-polarized systemic inflammation observed obesity-associated asthma is unknown. We studied epigenome-wide peripheral blood mononuclear cells (PBMCs) from 8 obese asthmatic pre-adolescent children and compared it to PBMCs with alone, obesity alone healthy controls. Differentially methylated loci implicated certain biologically relevant molecules pathways. had distinctive...
The Smchd1 gene encodes a large protein with homology to the SMC family of proteins involved in chromosome condensation and cohesion. Previous studies have found that has an important role CpG island (CGI) methylation on inactive X (Xi) stable silencing some Xi genes. In this study, using genome-wide expression analysis, we showed is required for around 10% genes Xi, apparently independent CGI hypomethylation, and, moreover, these nonrandomly occur clusters. Additionally, at cluster four...
DNA mutational events are increasingly being identified in autism spectrum disorder (ASD), but the potential additional role of dysregulation epigenome pathogenesis condition remains unclear. The is interest as a possible mediator environmental effects during development, encoding cellular memory reflected by altered function progeny cells. Advanced maternal age (AMA) associated with an increased risk having child ASD for reasons that not understood. To explore whether AMA involves covert...
Abstract Male and female brains differ significantly in both health disease, yet the brain has been understudied. Sex-hormone fluctuations make particularly dynamic are likely to confer female-specific risks for neuropsychiatric disorders. The molecular mechanisms underlying nature of structure function unknown. Here we show that neuronal chromatin organization ventral hippocampus mouse fluctuates with oestrous cycle. We find organizational changes associated transcriptional activity genes...
DNA methylation patterns in the genome both reflect and help to mediate transcriptional regulatory processes. The digital nature of methylation, present or absent on each allele, makes this assay capable quantifying events subpopulations cells, whereas genome-wide chromatin studies lack same quantitative capacity. Testing throughout is possible using whole-genome bisulfite sequencing (WGBS), but high costs associated with have made it impractical for involving more than limited numbers...
Although clear cell renal carcinoma (ccRCC) has been shown to result in widespread aberrant cytosine methylation and loss of 5-hydroxymethylcytosine (5hmC), the prognostic impact therapeutic targeting this epigenetic aberrancy not fully explored. Analysis 576 primary ccRCC samples demonstrated that 5hmC was strongly associated with aggressive clinicopathologic features an independent adverse factor. Loss also predicted reduced progression-free survival after resection nonmetastatic disease....
Cellular differentiation is marked by temporally and spatially coordinated gene expression regulated at multiple levels. DNA methylation represents a universal mechanism to control chromatin organization its accessibility. Cytosine of CpG dinucleotides regulates binding methylation-sensitive DNA-binding transcription factors within regulatory regions transcription, including promoters distal enhancers. Ocular lens an advantageous model system examine these processes as comprises only two...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProstaglandin synthesis. 16. The three-component coupling synthesis of prostaglandinsM. Suzuki, A. Yanagisawa, and Ryori. NoyoriCite this: J. Am. Chem. Soc. 1988, 110, 14, 4718–4726Publication Date (Print):July 1, 1988Publication History Published online1 May 2002Published inissue 1 July 1988https://pubs.acs.org/doi/10.1021/ja00222a033https://doi.org/10.1021/ja00222a033research-articleACS PublicationsRequest reuse permissionsArticle...
Optical reflectivity spectra of (La, Sr)2CuO4 and YBa2Cu3Oy have been measured over a wide spectral range as high 40 eV. Although overall features the observed are explained well by results available band calculations, differ significantly from calculation in low-energy region below 5 eV, where spectrum would presumably be dominated transitions between states involving Cu 3d O 2p or charge-transfer (CT) excitation states. In contrast to calculation, absorption that probably corresponds Cu–O...