A5042645070- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Neonatal Respiratory Health Research
- RNA modifications and cancer
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Genetic Syndromes and Imprinting
- Infant Nutrition and Health
- Genomics and Chromatin Dynamics
- Hemoglobinopathies and Related Disorders
- Complement system in diseases
- Erythrocyte Function and Pathophysiology
- Immune cells in cancer
- Online Learning and Analytics
- Effects of Radiation Exposure
- Intergenerational Family Dynamics and Caregiving
- Childhood Cancer Survivors' Quality of Life
- Sex and Gender in Healthcare
- Health, Environment, Cognitive Aging
- Voice and Speech Disorders
- Cancer, Stress, Anesthesia, and Immune Response
- Platelet Disorders and Treatments
- Stress Responses and Cortisol
- Lipid metabolism and biosynthesis
- Family Support in Illness
- Infant Development and Preterm Care
Albert Einstein College of Medicine
2008-2025
Montefiore Medical Center
2021-2025
British Heart Foundation
2015-2021
University of Edinburgh
2012-2021
Queen's Medical Centre
2020-2021
The Queen's Medical Research Institute
2012-2018
Foundation Center
2015
University of Delhi
2015
University of Colorado Denver
2013
University of Cambridge
2012
The distribution of cytosine methylation in 6.2 Mb the mouse genome was tested using cohybridization genomic representations from a methylation-sensitive restriction enzyme and its methylation-insensitive isoschizomer. This assay, termed HELP ( H paII tiny fragment E nrichment by L igation-mediated P CR), allows both intragenomic profiling intergenomic comparisons methylation. profile shows most to be contiguous methylated sequence with occasional clusters hypomethylated loci, usually but...
In addition to the genetic constitution inherited by an organism, developmental trajectory and resulting mature phenotype are also determined mechanisms acting during critical windows in early life that influence establish stable patterns of gene expression. This is crux origins health disease hypothesis suggests undernutrition gestation infancy predisposes ill later life. The periconceptional maternal micronutrient supplementation might affect fetal genome-wide methylation within promoters...
Intrauterine exposures mediated by maternal diet may affect risk of cardiovascular disease, obesity, and type 2 diabetes. Recent evidence, primarily from animal studies observational data in humans, suggests that the epigenome can be altered during periconceptional period these programming events underlie later disease risk. A randomized controlled trial micronutrient supplementation The Gambia, where seasonal nutritional variations fetal growth postnatal outcomes, provided a unique...
Cytosines at cytosine-guanine (CG) dinucleotides are the near-exclusive target of DNA methyltransferases in mammalian genomes. Spontaneous deamination methylcytosine to thymine makes methylated cytosines unusually susceptible mutation and consequent depletion. The loci where CG remain relatively enriched, presumably due their unmethylated status during germ cell cycle, have been referred as CpG islands. Currently, islands solely defined by base compositional criteria, allowing annotation any...
Early-life stress (ELS) is known to be associated with an increased risk of neuropsychiatric and cardiometabolic disease in later life. One the potential mechanisms underpinning this through effects on epigenome, particularly changes DNA methylation. Using a well-phenotyped cohort 83 men from Helsinki Birth Cohort Study, who experienced ELS form separation their parents during childhood, group matched controls, we performed genome-wide analysis methylation peripheral blood. We found no...
Lasting effects of adversity, such as exposure to childhood adversity (CA) on disease risk, may be embedded via epigenetic mechanisms but findings from human studies investigating the main measures, including DNA methylation (DNAm), are inconsistent. Studies in perinatal tissues indicate that variability DNAm at birth is best explained by joint genotype and prenatal environment. Here, we extend these analyses postnatal stressors. We investigated contribution CA, cis (G), their additive (G +...
Representations of the genome can be generated by selection a subpopulation restriction fragments using ligation-mediated PCR. Such representations form basis for number high-throughput assays, including HELP assay to study cytosine methylation. We find that data analysis is complicated not only PCR amplification heterogeneity but also complex and variable distribution To address this, we created an analytical pipeline novel normalization approach improves concordance between...
Background: Preterm birth associates with a substantially increased risk of later cardiovascular disease and neurodevelopmental disorders. Understanding underlying mechanisms will facilitate the development screening intervention strategies to reduce risk. Changes in DNA methylation have been proposed as one mechanism linking early environment We tested hypothesis that preterm altered genes encoding insulin-like growth factor 2 (IGF2) FK506 binding protein 5 (FKBP5), which appear...
Lung carcinogenesis is causally linked to cigarette smoking, in part by epigenetic changes. We tested whether accumulated change smokers apparent bronchial basal cells as of origin squamous cell carcinoma. Using an EM-seq platform covering 53.8 million CpGs (96% the entire genome) at average 7.5 sequencing reads per CpG site a single base resolution, we evaluated cytology-normal bronchoscopically brushed from situ tobacco smoke-exposed 'bronchial epithelial field' and isolated short-term...
Early life exposure to adverse environments affects cardiovascular and metabolic systems in the offspring. These programmed effects are transmissible a second generation through both male female lines, suggesting germline transmission. We have previously shown that prenatal overexposure synthetic glucocorticoid dexamethasone (Dex) rats reduces birth weight first (F1), phenotype which is transmitted (F2), particularly line. hypothesize Dex developing germ cells, resulting alterations DNA...
Altered placental function as a consequence of aberrant imprinted gene expression may be one mechanism mediating the association between low birth weight and increased cardiometabolic disease risk. Imprinted is regulated by epigenetic mechanisms, particularly DNA methylation (5mC) at differentially methylated regions (DMRs). While 5-hydroxymethylcytosine (5hmC) also present DMRs, many techniques do not distinguish 5mC 5hmC. Using human samples, we show that CDKN1C associates with weight....
Basal cells (BCs) are bronchial progenitor/stem that can regenerate injured airway that, in smokers, may undergo malignant transformation. As a model for early stages of lung carcinogenesis, we set out to characterize cytologically normal BC outgrowths from never-smokers and ever-smokers without cancers (controls), as well the epithelial "field" with anatomically remote cancers, including adenocarcinoma (LUAD) squamous cell carcinoma (LUSC) (cases).
Glucocorticoids are widely used in threatened preterm labor to promote maturation many organ systems babies and have significant beneficial effects on morbidity mortality. We performed transcriptional profiling fetal liver a rat model of prenatal glucocorticoid exposure identified marked gene expression changes heme biosynthesis, utilization, degradation pathways late gestation. These associated with alterations DNA methylation reduction hepatic concentration. There were no persistent...
Massive Open Online Course (MOOC) is an emerging method in online education. Sakai a popular open source platform widely used by e-learning providers to offer MOOC. provides set of collaborative tools for teachers, learners and researchers support teaching, learning research. For using Sakai, the provider needs customize adapt interface like, banner, logo, background color etc. suit their requirement. This paper presents development tool named SkinChanger that facilitates customizing...