A5031748666- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Renal and related cancers
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Histone Deacetylase Inhibitors Research
- Cancer Genomics and Diagnostics
- Genetic Syndromes and Imprinting
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Spaceflight effects on biology
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Radioactivity and Radon Measurements
- Xenotransplantation and immune response
- Reproductive biology and impacts on aquatic species
- Prenatal Screening and Diagnostics
- Mesenchymal stem cell research
The University of Tokyo
2023-2024
Tokyo University of Science
2019-2024
University of Tsukuba
2021-2023
Tokyo Medical and Dental University
2022-2023
RIKEN BioResource Research Center
2011-2021
University of Yamanashi
2012-2021
Takeda (Japan)
2014-2017
RIKEN Center for Computational Science
2007-2013
Institute of Livestock and Grassland Science
2010-2012
National Agriculture and Food Research Organization
2011
Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive their scientific medical utilities might prove limited. To evaluate characteristics of NT-ESCs, we established more than 150 NT-ESC lines adult somatic several mouse strains. Here, show that these were able...
Although the somatic cloning technique has been used for numerous applications and basic research of reprogramming in various species, extremely low success rates have plagued this a decade. Further mice, "clonable" strains limited to mainly hybrid F1 such as B6D2F1. Recently, we established new efficient using trichostatin A (TSA) which leads 2-5 fold increase mouse B6D2F1 cumulus cells. To further test validity TSA technique, tried clone adult ICR mouse, an outbred strain, never directly...
Since the birth of Cumulina, first mouse clone produced by somatic cell nuclear transfer (SCNT), success rate cloning in mice has been extremely low compared with other species and most inbred strains have never cloned. Recently, our laboratory found that treatment SCNT embryos trichostatin A, a histone deacetylase inhibitor (HDACi), improved full-term development B6D2F1 clones significantly. However, this was not effective for strains. Here, we show time treating another HDACi, scriptaid,...
Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient. Most SCNT-generated embryos die after implantation because of unidentified, complex epigenetic errors in the process postimplantation embryonic development. Here we identify most upstream level dysfunction leading to impaired development clones using RNAi against Xist, a gene responsible for X chromosome inactivation (XCI). A prior injection Xist-specific siRNA into reconstructed oocytes efficiently corrected...
The aim of the present study was to assess effects L-carnitine, an enhancer lipid metabolism and mitochondrial activity, during in vitro maturation (IVM) on nuclear fertilisation porcine follicular oocytes subsequent embryo development. Mitochondrial functions, intracellular content reactive oxygen species (ROS) levels were also investigated. L-carnitine supplementation 0.6-5mgmL(-1) concentration IVM significantly improved (P<0.05) rates metaphase-II (MII) stage compared with control;...
Cloning animals by nuclear transfer provides an opportunity to preserve endangered mammalian species. However, it has been suggested that the “resurrection” of frozen extinct species (such as woolly mammoth) is impracticable, no live cells are available, and genomic material remains inevitably degraded. Here we report production cloned mice from bodies kept at −20 °C for up 16 years without any cryoprotection. As all were ruptured after thawing, used a modified cloning method examined nuclei...
Since the first mouse clone was produced by somatic cell nuclear transfer, success rate of cloning in mice has been extremely low. Some histone deacetylase inhibitors, such as trichostatin A and scriptaid, have improved full-term development clones significantly, but mechanisms allowing for this are unclear. Here, we found that two other specific suberoylanilide hydroxamic acid oxamflatin, could also reduce apoptosis blastocysts, improve cloned mice, increase establishment transfer-generated...
Significance Radiation on the International Space Station (ISS) is more than 100 times stronger at Earth’s surface, and levels that can cause DNA damage in somatic cell nuclei. The to offspring caused by this irradiation germ cells has not been examined, however. Here we preserved mouse spermatozoa ISS for 9 mo. Although sperm was slightly damaged during space preservation, it could be repaired oocyte cytoplasm did impair birth rate or normality of offspring. Our results demonstrate...
Intra-embryo genome editing by CRISPR/Cas9 enables easy generation of gene-modified animals non-homologous end joining (NHEJ)-mediated frameshift mutations or homology-directed repair (HDR)-mediated point mutations. However, large modifications, such as gene replacement fusions, are still difficult to introduce in embryos without costly micromanipulators. Moreover, micromanipulation techniques for intra-embryo have been established only a small set animals. To overcome these issues, we...
Autologous skin grafting is a standard treatment for defects such as burns. No artificial substitutes are functionally equivalent to autologous grafts. The cultured epidermis lacks the dermis and does not engraft deep wounds. Although reconstituted skin, which consists of epidermal cells on synthetic dermal substitute, can wounds, it requires wound bed be well-vascularized appendages. In this study, we successfully generate complete grafts with pluripotent stem cell-derived appendages p63...
Nuclear transfer can be used to generate embryonic stem cell lines from somatic cells, and these have great potential in regenerative medicine. However, it is still unclear whether any individual or type such lines. Here, we tested seven different male female mouse genotypes three types as sources of nuclei determine the efficiency establishing nuclear Lines were successfully established all sources. Cumulus F1 showed a significantly higher cumulative establishment rate reconstructed oocytes...
Recently, ES cell lines were established from single blastomeres taken eight-cell embryos in mice and humans with success rates of 4% 2%, respectively, which suggests that the method could be used regenerative medicine to reduce ethical concerns over harm embryos. However, those studies other cells as supporting cells. Here, we report a simple highly efficient establishing mouse blastomeres, are simply plated onto feeder layer embryonic fibroblasts modified medium. A total 112 two-cell...
Oocyte growth, maturation, and activation are complex processes that include transcription, heterochromatin formation, chromosome condensation decondensation, two consecutive separations, genomic imprinting. The objective of this study was to investigate changes in histone H3 modifications relation chromatin/chromosome morphology pig oocytes during their activation. During the growth phase, acetylated at lysines 9, 14, 18 (K9, K14, K18), became methylated K9 when follicles developed antral...
We examined the effects of treatment with histone deacetylase inhibitors (HDACi), trichostatin A (TSA) and scriptaid (SCR), on blastocyst formation rate in bovine somatic cell nuclear transferred (SCNT) embryos derived from fibroblast cells. Three lines (L1, L2 L3) were used as donors to produce SCNT L3 embryos, respectively). In Experiment 1, we compared vitro developmental competence L1 treated various concentrations TSA for different time periods following chemical activation. Embryos 5...
Animals cloned by somatic cell nuclear transfer (SCNT) provide a unique model for understanding the mechanisms of epigenetic reprograming to state totipotency. Though many phenotypic abnormalities have been demonstrated in animals, underlying are not well understood. In this study, we performed transcriptome-wide allelic expression analyses brain and placental tissues mice. We found that Gab1, Sfmbt2 Slc38a4 showed loss imprinting all mice analyzed, which might be involved placentomegaly...
The current success rate of cloned mice from adult somatic cell nuclei is very low, whereas it relatively high for ES nuclei. In this experiment, we examined whether the cloning cells could be improved via nuclear transfer embryonic stem (ntES cells) established We obtained 11 and 68 ntES lines 7 mice, 41 were Unexpectedly, overall in series was no better than when using Interestingly, full-term produced only two individuals, but not by direct cells, vice versa. Ultimately, able to obtain...