Human cytomegalovirus (CMV), a herpesvirus that causes congenital disease and opportunistic infections in immunocompromised individuals, encodes functions facilitate efficient viral propagation by altering host cell behavior. Here we show CMV blocks apoptosis mediated death receptors mitochondria-localized inhibitor of apoptosis, denoted vMIA, capable suppressing induced diverse stimuli. product the UL37 gene, inhibits Fas-mediated at point downstream caspase-8 activation Bid cleavage but...

The aim of the present study was to assess effects L-carnitine, an enhancer lipid metabolism and mitochondrial activity, during in vitro maturation (IVM) on nuclear fertilisation porcine follicular oocytes subsequent embryo development. Mitochondrial functions, intracellular content reactive oxygen species (ROS) levels were also investigated. L-carnitine supplementation 0.6-5mgmL(-1) concentration IVM significantly improved (P<0.05) rates metaphase-II (MII) stage compared with control;...

An important step in predicting error prone modules a project is to construct the prediction model by using training data of that project, but resulting depends on data. Therefore it difficult apply other projects. The consists metrics and bug data, these should be prepared for each project. Metrics can computed metric tools, not so easy collect In this paper, we try reuse generated model. By which are from C++ Java projects, have evaluated possibility applying model, based one proposed...

We examined the effects of treatment with histone deacetylase inhibitors (HDACi), trichostatin A (TSA) and scriptaid (SCR), on blastocyst formation rate in bovine somatic cell nuclear transferred (SCNT) embryos derived from fibroblast cells. Three lines (L1, L2 L3) were used as donors to produce SCNT L3 embryos, respectively). In Experiment 1, we compared vitro developmental competence L1 treated various concentrations TSA for different time periods following chemical activation. Embryos 5...

A single-crystal β-Ga2O3 substrate was directly attached to a polycrystalline SiC (poly-SiC) using surface-activated-bonding method enhance heat extraction from devices. The effective thermal conductivity of the n+-Ga2O3/n+-poly-SiC bonded and electrical resistance at heterointerface were characterized by periodic heating radiation thermometry analyzing vertical current–voltage characteristics, respectively. Small resistances interface demonstrated strong prospects substrates for...

There is growing evidence that glutamatergic signaling may be involved in major depressive disorder (MDD). In regard to peripheral blood glutamate changes MDD, inconsistent findings have been reported. The purpose of the present study was evaluate whether levels differed between MDD patients and control participants.We conducted a systematic review meta-analysis 12 association studies total 529 590 controls. Subsequently, we subgroup analyses meta-regression analysis examine sources...

Capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) is a comprehensive, quantitative, and high throughput tool used to analyze metabolite profiles. In the present study, we CE-TOFMS profile metabolites found in blood plasma of 33 medication-free patients with major depressive disorder (MDD) non-psychiatric control subjects. We then investigated changes which occurred levels during an 8-week treatment period. The MDD subjects showed significant differences their mean...

Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay provide a quality parameter for bull semen based on detection sperm damage. Fresh collected two Japanese Black bulls (A, B) several times over course year, and percentage TUNEL-positive spermatozoa (sperm TUNEL index) determined. Individual differences in were detected...

To obtain the data concerning death losses due to stillbirth, neonatal and diseases in cloned cattle derived from somatic cell nuclear transfer (SCNT) their progeny produced by Japanese institutions, a nationwide survey was carried out July-August, 2006. As result, lifetime 482 SCNT (97.5% of country at that time) 202 were accumulated loss these analyzed. Although 1/3 delivered calves died during perinatal period stillbirth death, incidence surviving more than 200 days after birth seems be...

The aim of this study was to examine the effects bovine follicular fluid (bFF) on mitochondrial activity in vitro-matured (IVM) oocytes and assess its importance for fertilisation embryo development. Bovine were subjected IVM medium supplemented either with polyvinylpyrrolidone, serum albumin, calf or bFF. Nuclear maturation, cumulus expansion, distribution ATP content compared between groups along subsequent vitro (IVF) Compared other supplements, bFF generated significantly enhanced...

ABSTRACT Objective Refeeding in patients with anorexia nervosa (AN) is associated a risk of refeeding syndrome, which disruption metabolism variety features including hypophosphatemia. We evaluated the factors for hypophosphatemia (RH) during nutritional replenishment Japanese AN. Methods retrospectively examined clinical data 99 female inpatients (mean age 30.9 ± 10.7 years; range, 9 − 56 years). Results RH (phosphate &lt; 2.3 mg/dL) occurred within 4.8 3.7 days hospital admission and was...

We examined the effects of source recipient oocytes and timing fusion activation on development competence bovine nuclear transferred (NT) embryos derived from fresh cumulus cells isolated immediately after collection by ovum pickup (OPU). As cytoplasts, we used in vivo-matured collected hormone-treated heifers OPU, or vitro-matured slaughterhouse-derived ovaries. NT were chemically activated (simultaneous activation, FA) 2 h (delayed DA) fusion. When as rate to blastocyst stage produced DA...

Background Suicide has been a leading cause of death among young adult populations in Japan. The aim this study was to predict major depressive episodes (MDEs) and suicide-related ideation university students using the Patient Health Questionnaire-9 (PHQ-9) Temperament Character Inventory (TCI). Methods subjects were 2194 who completed PHQ-9 TCI 1st year (T1) 4th (T2) university. Multiple logistic regression analysis performed MDEs at T2. Moreover, recursive partitioning analyses conducted...

ABSTRACT To obtain data concerning the survival of embryos and calves derived from somatic cell nuclear transfer (SCNT) in Japan, a nationwide survey was carried out April, 2009. As result, 3264 embryo transfers (ETs) with SCNT which produced 301 were accumulated their analyzed. The present revealed that rates transferred bovine had not improved over decade (1998–2007). A remarkable feature pregnancies high incidence spontaneous abortions. When divided by occurrence spongiform encephalopathy...

Objectives Vascular endothelial growth factor (VEGF) is thought to be involved in the pathophysiology of mood disorders and target antidepressants. The aim this study was elucidate molecular effects lithium on VEGF expression by using leukocytes healthy subjects patients with bipolar disorder. Methods Eight male participated first study. Lithium prescribed for 2 weeks, enough reach therapeutic serum concentration. Leukocyte counts concentrations were determined at baseline, 1- 2-week...

We examined the influence of recipient oocytes on in vitro development, oxygen consumption, and gene expression resulting cloned bovine embryos. Oocytes derived from slaughterhouse ovaries ovum pickup (OPU)-derived were used as cytoplasts for production A series OPU sessions was conducted Holstein cows without follicular growth treatment (FGT). In same cows, we then performed dominant follicle ablation subsequently administered follicle-stimulating hormone prostaglandin F2α with controlled...

Purpose: The purpose of the present study was to examine clinical factors related social function in people with schizophrenia. Patients and methods: participants were 55 stabilized outpatients Their mean age 39.36 (SD =10.65) years. Social assessed using Quality Life Scale (QLS). Cognitive evaluated Measurement Treatment Research Improve Cognition Schizophrenia Consensus Battery (MCCB). Clinical symptoms Positive Negative Syndrome (PANSS), Calgary Depression for Schizophrenia, Drug-Induced...

: Suicide is a serious social health problem, especially among young adults. This study aimed to investigate the link between self-esteem, anxiety, hopelessness, and suicidal ideation Japanese university students. Data were collected from 322 students (Mage = 21; 61.8% male) who visited Hokkaido University Health Care Center in Japan for psychiatric consultation. Participants completed questionnaires including Rosenberg Selfesteem Scale, Liebowitz Social Anxiety Beck Hopelessness Patient...

In order to obtain control data on the temperature sense (warm and cool threshold values) of fingertips, relationships between room either skin temperature, warm or middle fingertips were investigated in healthy subjects (6 males), using our thermo-esthesiometer. The changes a sigmoidal response with variation temperature. A point inflection for this was observed at 15 degrees C, which greatest standard deviation occurred. thresholds, other hand, also affected by variations Warm bore linear...

Objectives To elucidate the molecular effects of lithium, we studied global gene expression changes induced by lithium in leukocytes from healthy subjects. Methods Eight male subjects participated this study. Lithium was prescribed for weeks to reach a therapeutic serum concentration. Leukocyte counts and concentrations were determined at baseline (before medication), after 1 2 medication stopping medication. Gene profiling performed each time point using Agilent G4112F Whole Human Genome...