A5033394148- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Leaf Properties and Growth Measurement
- RNA modifications and cancer
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Plant responses to water stress
- Plant Physiology and Cultivation Studies
- Insect-Plant Interactions and Control
- Remote Sensing in Agriculture
- Plant tissue culture and regeneration
- Physiological and biochemical adaptations
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Plant Water Relations and Carbon Dynamics
- Growth and nutrition in plants
- Tree Root and Stability Studies
Institut Jean-Pierre Bourgin
2013-2024
Université Paris-Saclay
2018-2024
AgroParisTech
2013-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Centre National de la Recherche Scientifique
2018-2019
Increased phenotyping accuracy and throughput are necessary to improve our understanding of quantitative variation be able deconstruct complex traits such as those involved in growth responses the environment. Still, only a few facilities known handle individual plants small stature for non-destructive, real-time phenotype acquisition from grown precisely adjusted variable experimental conditions. Here, we describe Phenoscope, high-throughput platform that has unique feature continuously...
Abstract There is renewed interest in whether environmentally induced changes phenotypes can be heritable. In plants, heritable trait variation occur without DNA sequence mutations through epigenetic mechanisms involving methylation. However, it remains unknown this alternative system of inheritance responds to environmental and if provide a rapid way for plants generate adaptive phenotypic variation. To assess potential transgenerational effects by the environment, we subjected four natural...
Abstract Understanding plant responses to individual stresses does not mean that we understand real-world situations, where usually combine and interact. These interactions arise at different levels, from stress exposure the molecular networks of response. Here, built an in-depth multiomic description mild water (W) nitrogen (N) limitations, either individually or combined, among 5 genetically Arabidopsis (Arabidopsis thaliana) accessions. We highlight dynamics in response through...
One of the main outcomes quantitative genetics approaches to natural variation is reveal genetic architecture underlying phenotypic space. Complex architectures are described as including numerous loci (or alleles) with small-effect and/or low-frequency in populations, interactions background, environment or age. Linkage association mapping strategies will be more less sensitive this complexity, so that we still have an unclear picture its extent. By combining high-throughput phenotyping...
Abstract Modern plant breeding, such as genomic selection and gene editing, is based on the knowledge of genetic architecture desired traits. Quantitative trait loci (QTL) analysis, which combines high throughput phenotyping genotyping segregating populations, a powerful tool to identify these determinants decipher underlying mechanisms. However, meiotic recombination, shuffles information between generations, limited: Typically only one two exchange points, called crossovers, occur pair...
Abstract Investigating the evolution of complex phenotypes and underlying molecular bases their variation is critical to understand how organisms adapt environment. Applying classical quantitative genetics on a segregating population derived from Can-0xCol-0 cross, we identify MADS-box transcription factor FLOWERING LOCUS M (FLM) as player phenotypic in plant growth color. We show that allelic at FLM modulates strategy along leaf economics spectrum, trade-off between resource acquisition...
Drought response in plants is complex, involving integration across a range of physiological processes. However, our knowledge how different aspects drought are linked at the genetic level limited. We investigated multi-trait adaptation Arabidopsis thaliana from Cape Verde Islands (CVI). Using high-throughput phenotyping platform that minimizes spatial heterogeneity, we measured variation rosette area, growth rate, leaf color, water-use efficiency (WUE), and stomatal patterning under...
Adjustment to energy starvation is crucial ensure growth and survival. In Arabidopsis thaliana (Arabidopsis), this process relies in part on the phosphorylation of circadian clock regulator bZIP63 by SUCROSE non-fermenting RELATED KINASE1 (SnRK1), a key mediator responses low energy. We investigated effects mutations plant carbon (C) metabolism growth. Results from phenotypic, transcriptomic metabolomic analysis mutants prompted us investigate starch accumulation pattern expression genes...
Abstract Energy production and metabolism are intimately linked to ecological environmental constraints across the tree of life. In plants, which depend on sunlight produce energy, link between primary environment is especially strong. By governing CO2 uptake for photosynthesis transpiration, leaf pores, or stomata, couple energy determine productivity water-use efficiency (WUE). Although evolution known tune physiological traits local environment, we lack knowledge specific links molecular...
Abstract Soil salinization poses a significant threat to crop production impacting one fifth of all cultivated land. The Cape Verde Islands are located 600 km from the coast Africa and characterized by high salinity soils inland water sources. In this study we find that Arabidopsis thaliana plants native these islands accumulate metabolite protects them salt stress. We partially as glucuronyl-mannose. ability produce glucuronyl-mannose evolved independently in two different same archipelago...
Summary Whether environmentally induced changes in phenotypes can be heritable is a topic with revived interest, part because of observations plants that trait variation occur without DNA sequence mutations. This other system inheritance, called transgenerational epigenetics, typically involves differences methylation are stable across multiple generations. However, it remains unknown if such responds to environmental and could therefore provide rapid way for generate adaptive phenotypic...
To fully understand gene regulation, it is necessary to have a thorough understanding of both the transcriptome and enzymatic RNA-binding activities that shape it. While many RNA-Seq-based tools been developed analyze transcriptome, most only consider abundance sequencing reads along annotated patterns (such as genes). These annotations are typically incomplete, leading errors in differential expression analysis. address this issue, we present DiffSegR - an R package enables discovery...
Abstract Sugars, produced through photosynthesis, are at the core of all organic compounds synthesized and used for plant growth response to environmental changes. Their production, transport, utilization highly regulated integrated throughout life cycle. The maintenance sugar partitioning between different subcellular compartments cells is important in adjusting photosynthesis performance abiotic constraints. Here, we investigated, Arabidopsis, consequences disruption four genes coding...
Abstract Loss of DNA methylation over transposable elements (TEs) can affect neighboring genes and be epigenetically inherited in plants, yet the determinants significance this additional system inheritance are unknown. Here, we demonstrate at thousands TE loci across Arabidopsis thaliana genome, that experimentally-induced hypomethylation transmitted transgenerationally reveal role small RNAs derived from related copies counteracting transmission. Using data >700 strains collected...
Abstract Sugars, produced through photosynthesis, are at the core of all organic compounds synthesized and used for plant growth their response to environmental changes. Their production, transport, utilization highly regulated integrated throughout life cycle. The maintenance sugar partitioning between different subcellular compartments cells is important in adjusting photosynthesis performance abiotic constraints. We investigated consequences disruption four genes coding SWEET transporters...
ABSTRACT Investigating the evolution of complex phenotypes and underlying molecular bases their variation is critical to understand how organisms adapt environment. We used leaf growth as a model trait it highly integrative internal external cues relies on functions at different levels plant organization. Applying classical quantitative genetics recombinant inbred line population derived from Can-0 x Col-0 cross, we identified MADS-box transcription factor FLOWERING LOCUS M ( FLM ) player...
ABSTRACT To fully understand gene regulation, it is necessary to have a thorough understanding of both the transcriptome and enzymatic RNA-binding activities that shape it. While many RNA-Seq-based tools been developed analyze transcriptome, most only consider abundance sequencing reads along annotated patterns (such as genes). These annotations are typically incomplete, leading errors in differential expression analysis. address this issue, we present DiffSegR - an R package enables...