A5000166558- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Plant Taxonomy and Phylogenetics
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Food composition and properties
- Garlic and Onion Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Genetic and Mutation Studies
- Phytase and its Applications
- Legume Nitrogen Fixing Symbiosis
- Genetics and Plant Breeding
- Transgenic Plants and Applications
- Peanut Plant Research Studies
- Plant nutrient uptake and metabolism
- Agriculture, Plant Science, Crop Management
- Crop Yield and Soil Fertility
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Plant and fungal interactions
- Nitrogen and Sulfur Effects on Brassica
- Plant Pathogens and Resistance
Osaka Kyoiku University
2010-2024
Plant Industry
2004-2011
Leibniz Institute of Plant Genetics and Crop Plant Research
2011
University of Lucknow
2004
Central Institute of Medicinal and Aromatic Plants
2004
Akita Prefectural University
2004
Commonwealth Scientific and Industrial Research Organisation
2004
Japan Science and Technology Agency
2004
University of Delhi
2001
Kansas State University
1990-1993
Common wheat, Triticum aestivum, is an allohexaploid species consisting of three different genomes (A, B, and D). The were simultaneously discriminated with colors. Biotinylated total genomic DNA the diploid A genome progenitor urartu, digoxigenin-labeled D Aegilops squarrosa, nonlabeled one possible B progenitors Ae. speltoides hybridized in situ to metaphase chromosome spreads aestivum cv. Chinese Spring. For detection, only two fluorochromes, fluorescein rhodamine, used. A, detected by...
In situ hybridization in conjunction with deletion mapping was used to physically map the 5S rRNA multigene family Triticum aestivum L. cv. 'Chinese Spring.' Twelve loci were mapped on chromosomes of homoeologous group 1 (arms 1AS, BS, and 1DS) 5 5AS, 5BS, 5DS). The (fraction distance from centromere) at positions 0.77, 0.96, 0.76, 0.63, 0.64 arms 1DS, 5DS, respectively. locus 1BS position ca. 0.5 satellite length. Deletions further chromosome bands 1AS12, 1BS32, 1DS22, 5AS22, 5BS22, 5DS22....
Whole genome duplication leads to autopolyploidy and brings about an increase in cell size, concentration of secondary metabolites enhanced cytosine methylation. The increased size offers a positive advantage polyploids for cell-surface-related activities, but there is differential response change body across species taxonomic groups. Although polyploidy has been very extensively studied, having genetic, ecological evolutionary implications, no report that underscores the significance native...
We are attempting to produce nucleus-cytoplasm hybrids in all possible combinations between the nuclei of twelve strains common wheat covering wide range its genetic variation, and cytoplasms 22 species Triticum Aegilops (including one synthetic amphiploid) representing almost basic genome types known two genera. Though their production is still far from completion, comparative studies under development indicated that great diversity cytoplasm exists among genera, can be classified into...
Asexual seed formation, or apomixis, in the Hieracium subgenus Pilosella is controlled by two dominant independent genetic loci, LOSS OF APOMEIOSIS (LOA) and PARTHENOGENESIS (LOP). We examined apomixis mutants that had lost function one both loci to establish their developmental roles during formation. In apomicts, sexual reproduction initiated first. Somatic aposporous initial (AI) cells differentiate near meiotic cells, pathway terminated as AI undergo mitotic embryo sac Seed initiation...
Abstract Genes and cDNAs for starch-branching enzyme II (SBEII) have been isolated from libraries constructed Aegilops tauschii wheat (Triticum aestivum) endosperm, respectively. One class of genes has termedwSBEII-DA1 encodes the N terminus reported an SBEII endosperm. On basis phylogenetic comparisons with other branching sequences, wSBEII-DA1 is considered to be a member SBEIIa class. ThewSBEII-DA1 gene consists 22 exons 4 21 being identical in length maize (Zea mays) SBEIIb gene, located...
Abstract The diploid-like chromosome pairing in polyploid wheat is controlled by the Ph1 (pairing homoeologous) gene that located on arm 5BL. By using a combination of cytogenetic and molecular techniques, we report physical location to submicroscopic region (Ph1 region) flanked breakpoints two deletions (5BL-1 ph1c) marked DNA probe (XksuS1). present distal breakpoint deletion 5BL-1 but proximal C-band 5BL2.1. Two other probes (Xpsr128 Xksu75) flank region-Xpsr128 being Xksu75 distal....
A technique for in situ hybridization is reported that can be used to detect barley chromatin wheat background using total genomic DNA as a probe. 1:2 ratio of biotin-labeled blocking (unlabeled, sheared) was sufficient reveal brownish labeled chromosome domains against bluish unlabeled metaphase, prophase, and interphase nuclei wheat-barley addition lines. Using this procedure, the behavior specific chromosomes analyzed prophase cells. In cells, 6H always associated with nucleolus. clone...
To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis plants, a genomic DNA fragment from Aegilops tauschii was introduced into ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, which endosperm starch is completely replaced by phytoglycogen. A. D genome donor wheat (Triticum aestivum), and effectively included gene for ISA1 (TaISA1) that encoded on genome. In TaISA1-expressing endosperm, phytoglycogen synthesis substantially synthesis, leaving only residual...
In order to obtain new information on the genome organization of Arachis ribosomal DNA, more particularly among A. hypogaea and its close relatives, distribution 18S-5.8S-26S 5S RNA gene families chromosomes 21 diploid tetraploid species, selected from six nine taxonomic sections, was analyzed by in situ hybridization with pTa71 (18S-5.8S-26S rDNA) pTa794 (5S clones. Two major rDNA loci intense signals were found nucleolus organizer regions (NOR) each species. addition extended at NORs, two...
C-banding patterns of the seven `Imperial' rye chromosomes and 11 derived telocentrics added to `Chinese Spring' were analyzed compared with in situ hybridization (ISH) using biotin labeled highly repetitive DNA sequences pSc119, pSc74, total genomic as probes. ISH analyses allow identification all individual most chromosome arms these A C-banded karyotype was constructed data on lengths, arm ratios, fraction lengths diagnostic C-bands are given following nomenclature system recently...
Abstract The LOSS OF APOMEIOSIS (LOA) locus is one of two dominant loci known to control apomixis in the eudicot Hieracium praealtum. LOA stimulates differentiation somatic aposporous initial cells after initiation meiosis ovules. Aposporous undergo nuclear proliferation close sexual megaspores, forming unreduced embryo sacs, and program ceases. LOA-linked genetic markers were used isolate 1.2 Mb LOA-associated DNAs from H. Physical mapping defined genomic region essential for function...
Summary Apomixis or asexual seed formation in Hieracium praealtum (Asteraceae) is controlled by two independent dominant loci. One of these, the LOSS OF APOMEIOSIS ( LOA ) locus, controls apomixis initiation, mitotic embryo sac (apospory) and suppression sexual pathway. The locus found near end a hemizygous chromosome surrounded extensive repeats extending along arm. Similar apomixis‐carrying structures have been some apomictic grasses, suggesting that repetitive sequences may be...