A5089839600- Food composition and properties
- Phytase and its Applications
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Rice Cultivation and Yield Improvement
- GABA and Rice Research
- Polysaccharides Composition and Applications
- Wheat and Barley Genetics and Pathology
- Probiotics and Fermented Foods
- Legume Nitrogen Fixing Symbiosis
- Plant Genetic and Mutation Studies
- Seed and Plant Biochemistry
- Plant nutrient uptake and metabolism
- Enzyme Production and Characterization
- Potato Plant Research
- Colorectal Cancer Screening and Detection
- Diet, Metabolism, and Disease
- Voice and Speech Disorders
- Bacterial Genetics and Biotechnology
- Soybean genetics and cultivation
- Diet and metabolism studies
- Polysaccharides and Plant Cell Walls
- Folate and B Vitamins Research
- Arsenic contamination and mitigation
- Food Industry and Aquatic Biology
Indian Institute of Rice Research
2021
Commonwealth Scientific and Industrial Research Organisation
2005-2020
Agriculture and Food
2017-2020
Health Sciences and Nutrition
2018
Plant Industry
2004-2017
Animal, Food and Health Sciences
2006-2012
ACT Government
2003-2012
Chris Hani Baragwanath Hospital
2000-2011
Alfred Mann Foundation
2005
University of Southern California
2005
Foods high in resistant starch have the potential to improve human health and lower risk of serious noninfectious diseases. RNA interference was used down-regulate two different isoforms starch-branching enzyme (SBE) II (SBEIIa SBEIIb) wheat endosperm raise its amylose content. Suppression SBEIIb expression alone had no effect on content; however, suppression both SBEIIa resulted containing >70% amylose. When grain fed rats a diet as wholemeal, several indices large-bowel function, including...
The roles of starch branching enzyme (SBE, EC 2.4.1.18) IIa and SBE IIb in defining the structure amylose amylopectin barley (Hordeum vulgare) endosperm were examined. Barley lines with low expression or IIb, both isoforms generated through RNA-mediated silencing technology. These enabled study role each these determining content, distribution chain lengths, frequency amylopectin. In where reduced by >80%, a high phenotype (>70%) was observed, while reduction either alone had minor impact on...
amylose extender (ae−) starches characteristically have modified starch granule morphology resulting from amylopectin with reduced branch frequency and longer glucan chains in clusters, caused by the loss of activity major branching enzyme (SBE), which maize endosperm is SBEIIb. A recent study ae− lacking SBEIIb protein (termed ae1.1 herein) showed that novel protein–protein interactions between enzymes biosynthesis amyloplast could explain phenotype mutant. The present examined an allelic...
Summary Resistant starch ( RS ), a type of dietary fibre, plays an important role in human health; however, the content most modern processed starchy foods is low. Cereal starch, when structurally manipulated through modified biosynthetic pathway to greatly increase amylose content, could be food source . Transgenic studies have previously revealed requirement simultaneous down‐regulation two branching enzyme SBE ) II isoforms both located on long arm chromosome 2, namely SBEII and b ,...
The sugary-2 mutation in maize (Zea mays L.) is a result of the loss catalytic activity endosperm-specific SS (starch synthase) IIa isoform causing major alterations to amylopectin architecture. present study reports biochemical and molecular analysis an allelic variant expressing catalytically inactive form SSIIa sheds new light on its central role protein-protein interactions determination starch granule proteome. mutant revealed two amino acid substitutions, one being highly conserved...
Studies in Arabidopsis and rice suggest that manipulation of starch synthase I (SSI) expression wheat may lead to the production grains with novel structure properties. This work describes suppression SSI using RNAi technology, which leads a low level enzymatic activity for developing endosperm, abundance protein inside granules mature grains. The amylopectin fraction from suppressed lines showed an increased frequency very short chains (degree polymerization, dp 6 7), lower proportion (dp...
Abstract Genes and cDNAs for starch-branching enzyme II (SBEII) have been isolated from libraries constructed Aegilops tauschii wheat (Triticum aestivum) endosperm, respectively. One class of genes has termedwSBEII-DA1 encodes the N terminus reported an SBEII endosperm. On basis phylogenetic comparisons with other branching sequences, wSBEII-DA1 is considered to be a member SBEIIa class. ThewSBEII-DA1 gene consists 22 exons 4 21 being identical in length maize (Zea mays) SBEIIb gene, located...
Previous research has not considered the effect of high amylose wheat noodles on postprandial glycaemia. The aim study is to investigate consumption glycaemia over 2-h periods by monitoring changes in blood glucose concentration and calculating total area under curve. Twelve healthy young adults were recruited a repeated measure randomised, single-blinded crossover trial compare consuming (180 g) containing 15%, 20% 45% Fasting concentrations taken via finger-prick samples. Postprandial at...
Dietary starch that escapes digestion in the small intestine may be quantitatively more important than dietary fiber as a substrate for fermentation. The products of fermentation have implications pathogenesis colorectal cancer and other diseases large bowel, which are uncommon Africans but high prevalence Western populations.Maize porridge is staple most blacks South Africa. Stale maize (high-resistant [HRS]) seems to induce greater bowel fresh (low-resistant [LRS]).In present study,...
Herein, optimized headspace solid phase microextraction with gas chromatography–tandem mass spectrometry (HS-SPME-GC-MS/MS) was used to estimate the 2-acetyl-1-pyrroline (2-AP) in raw and cooked rice samples of ten different traditional improved varieties. Furthermore, HS-SPME-GC-MS-based volatile profiling subjected untargeted analyses identify major odorants samples, understand chemical proximities among profiles. Results showed that 2-AP content remarkably increased compared raw. Among...
The present study was aimed at exploring the effect of soil application different concentrations orthophosphate (P) (0, 10, 20, 30, and 40 mg kg-1) on rice agronomic yield parameters, arsenic (As) species accumulation, polyphenol levels in grain grown under As spiked (10 kg-1). contents (As(V), (III), MMA DMA) polyphenols samples were estimated using LC-ICP-MS LC-MS/MS, respectively. P treatments significantly reduced toxic effects parameters such as root weight length, shoot spike straw,...
The role of starch branching enzyme-I (SBE-I) in determining structure the endosperm has been investigated. Null mutations SBE-I at A, B and D genomes wheat were identified Australian varieties by immunoblotting. By combining individual null through hybridisation, a double-null mutant wheat, which lacks isoforms SBE-I, was developed. Wheat mutants lacking all three generated from doubled haploid progeny cross between BD line Chinese Spring (CS) deletion A genome isoform. Comparison this to...
A line of wheat (Triticum aestivum L.), sgp-1, that does not express starch synthase II (SSII, also known as SGP-1) has previously been reported. In this study, F1 derived doubled haploid lines with homozygous wild type or mutant alleles for SGP-1 genes were identified from a cross between the original and Australian cultivar. Analysis granules showed in they are markedly distorted 15 days postanthesis during grain development. Starch branching patterns an increase proportion short chains...
Wheat starch is considered to have a low paste viscosity relative other starches. Consequently, wheat not preferred for many applications as compared high Increasing the of expected increase functionality range flour-based products in which texture an important aspect consumer acceptance (e.g., pasta, and instant yellow alkaline noodles). To understand molecular basis viscosity, we undertaken comprehensive structural rheological analysis starches from genetically diverse set genotypes,...