A5101734586- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related gene regulation
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- ATP Synthase and ATPases Research
- Mechanisms of cancer metastasis
- Plasmonic and Surface Plasmon Research
- Medical Imaging and Pathology Studies
- Plant Molecular Biology Research
- Caveolin-1 and cellular processes
- Ferroptosis and cancer prognosis
- Lipid Membrane Structure and Behavior
- Turfgrass Adaptation and Management
- Protein Tyrosine Phosphatases
- Biotin and Related Studies
- Cancer, Hypoxia, and Metabolism
Nanjing Medical University
2018-2025
Sichuan Agricultural University
2024
First People's Hospital of Chongqing
2024
Soochow University
2023
Second Affiliated Hospital of Soochow University
2023
University of Pennsylvania
2008-2021
Southeast University
2020
Jilin Medical University
2016-2018
Jilin University
2016-2018
University of California, Los Angeles
2015
Abstract Mounting evidences indicate that circular RNAs (circRNAs) have a vital role in human diseases, especially cancers. More recently, circHIPK3, particularly abundant circRNA, was proposed to be involved tumorigenesis. However, its colorectal cancer (CRC) has not been explored. In this study, we found circHIPK3 significantly upregulated CRC tissues and cell lines, at least part, due c-Myb overexpression positively correlated with metastasis advanced clinical stage. Moreover, Cox...
Recent studies indicate that circular RNA (circRNA) plays a pivotal role in cancer progression. Here, we sought to investigate its breast cancer. CircANKS1B (a circRNA originated from exons 5 8 of the ANKS1B gene, hsa_circ_0007294) was identified by RNA-sequencing and validated qRT-PCR Sanger sequencing. Clinical samples were used evaluate expression circANKS1B associations with clinicopathological features prognosis. Gain- loss-of-function experiments cell lines mouse xenograft models...
Colorectal cancer (CRC) is one of the leading causes tumor-related death worldwide, and its main cause distant metastasis. Methyltransferase-like 14(METTL14), a major RNA N6-adenosine methyltransferase, involved in tumor progression via regulating function. The goal study to uncover biological function molecular mechanism METTL14 CRC.Quantitative real-time PCR (qRT-PCR), western blot immunohistochemical (IHC) assays were employed detect SOX4 CRC cell lines tissues. functions demonstrated...
Mounting evidence demonstrates that long noncoding RNAs (lncRNAs) have critical roles during the initiation and progression of cancers. In this study, we report small nucleolar RNA host gene 1 (SNHG1) is involved in colorectal cancer progression.We analyzed sequencing data to explore abnormally expressed lncRNAs cancer. The effects SNHG1 on were investigated through vitro vivo assays (i.e., CCK-8 assay, colony formation flow cytometry EdU xenograft model, immunohistochemistry, western blot)....
Emerging studies suggest that long non-coding RNAs (lncRNAs) play crucial roles in colorectal cancer (CRC). Here, we report a lncRNA, SATB2-AS1, which is specifically expressed tissue and significantly reduced CRC. We systematically elucidated its functions possible molecular mechanisms LncRNA expression CRC was analyzed by RNA-sequencing RNA microarrays. The level of SATB2-AS1 tissues determined quantitative reverse transcription polymerase chain reaction (qRT-PCR) situ hybridization (ISH)....
Background: Exosomal circular RNAs (circRNAs) in peripheral blood are considered as emerging diagnostic biomarkers of cancers. Owing to the lack sensitive and specific biomarkers, a large number colorectal cancer (CRC) patients were diagnosed advanced stages leading high mortality. This study aimed identify circulating exosomal circRNAs novel CRC. Materials Methods: Candidate circRNA was selected by integrating analysis Gene Expression Omnibus (GEO) database with online program GEO2R. A...
Abnormal expression of long non-coding RNAs (lncRNAs) has been found in almost all human tumors, providing numerous potential diagnostic biomarkers, prognostic and therapeutic targets.
Increasing long non-coding RNAs (lncRNAs) have been reported to play key roles in the development and progression of various malignancies. ZNFX1 antisense RNA1 (ZFAS1) has be aberrant expression suggested as a tumor suppressor or oncogene many cancers. However, biological role underlying molecular mechanism ZFAS1, especially miRNA sponge which CRC remain largely unknown. We found that ZFAS1 was higher tissues, where it associated with poor overall survival (OS), we also showed upregulation...
Epigenetic alterations contributed to human carcinogenesis immensely. N6-methyladenosine (m6A) is one of the most preventive and abundant modifications on RNA molecules present in eukaryotes. However, biological function m6A methylation colorectal cancer (CRC) remains largely unclear. Here, we found that METTL14 was downregulated CRC tissues cell lines, closely correlated with overall survival (OS). knockdown significantly reduced levels total RNAs promoted growth metastasis, whereas...
Background: Colorectal cancer is one of the most common cancers worldwide usually associated with poor prognosis due to advanced stage when diagnosed. This study aimed investigate whether specific circulating exosomal miRNAs could act as biomarkers for early diagnosis colorectal cancer.Methods: A total 369 peripheral blood samples were included in this study. In discovery phase, miR-27a and miR-130a selected after synthetical analysis two GEO datasets TCGA database. The differential...
Background: Hepatocellular carcinoma (HCC) is one of the most aggressive cancers, with limited new diagnostic and therapeutic measures.This study aimed to investigate utility specific serum exosome lncRNAs as biomarkers for early diagnosis HCC.Methods: The relative expression levels eight selected in were evaluated by quantitative reverse transcription-PCR (qRT-PCR) training validation sets HCC patients matched healthy controls.Additionally, stability, specificity efficiency these determine...
MicroRNA-150-5p (miR-150-5p) has been implicated in tumor initiation and progression a variety of cancers. However, its roles colorectal cancer (CRC) remain largely unknown. In our study, decreased miR-150-5p expression CRC tissues was found to be associated with poor overall survival. Moreover, inhibited cell proliferation, migration, invasion angiogenesis vitro vivo, inhibitory effect could reversed by transfection vascular epithelial growth factor A (VEGFA) plasmid. Lastly, we...
Abstract MicroRNAs (miRNAs) are important class of functional regulators involved in human cancers development, including colorectal cancer (CRC). Exploring aberrantly expressed miRNAs may provide us with new insights into the initiation and development CRC by functioning as oncogenes or tumor suppressors. The aim our study is to discover expression pattern miR-1249 investigate its clinical significance well biological role progression. In study, we found that was markedly downregulated...
Clinically, one of the principal factors in failure advanced colorectal cancer (CRC) treatment is chemoresistance to 5-fluorouracil (5FU)-based chemotherapy. Although microRNA-375-3p (miR-375) considered a tumor suppressor multiple cancers, mechanism miR-375 regulation drug resistance CRC remains unclear. In this study, we investigated chemosensitivity 5FU from biological and clinical aspects. We found that was significantly downregulated tissues cell lines, low expression strongly...
Abstract As one of the most common cancers worldwide, colorectal cancer (CRC) causes a large number mortality annually. Aberrant expression microRNAs (miRNAs) is significantly associated with initiation and development CRC. Further investigations regarding regulatory mechanism miRNAs warranted. In this study, we discovered that miR-486-5p was remarkably downregulated in CRC, which partially results from higher DNA methylation promoter region detected by using methylation-specific PCR,...
Accumulating evidence suggests long noncoding RNAs (lncRNAs) play important roles in the initiation and progression of cancers. However, their functions chromophobe renal cell carcinoma (chRCC) are not fully understood.We analyzed expression profiles lncRNA, microRNA, protein-coding RNA, along with clinical information 59 primary chRCC patients collected from The Cancer Genome Atlas database to identify lncRNA biomarkers for prognosis. We also constructed an lncRNA-microRNA-mRNA coexpression...
Hepatocellular carcinoma (HCC), one of the most aggressive malignancies, ranks as fourth leading cause cancer-related deaths worldwide. Emerging evidence indicates that RNA N6-methyladenosine (m6A) plays a critical role in tumor progression. However, biological function YTHDF1 HCC remains unclear. Here, we found expression was strikingly elevated tissues and cell lines significantly associated with prognosis patients. Moreover, transcriptionally regulated by USF1 c-MYC HCC. Functional...
N6-methyladenosine (m6A) RNA methylation is the most prevalent modification of messenger RNAs (mRNAs) and catalyzed by a multicomponent methyltransferase complex (MTC), among which methyltransferase-like 3 (METTL3) METTL14 are two core molecules. However, METTL3 play opposite regulatory roles in hepatocellular carcinoma (HCC). Based on The Cancer Genome Atlas (TCGA) database Gene Expression Omnibus (GEO) database, we conducted multi-omics analysis HCC, including RNA-sequencing,...
Background: The lack of screening methods with high diagnostic utility leads to colorectal cancer (CRC) patients usually diagnosed in advanced stages which results mortality. This study aimed identify novel circulating miRNAs as biomarkers for the early detection CRC. Materials and Methods: Total 205 participants were enrolled this study. First, two dysregulated candidate selected after integrated analysis four GEO datasets. Then, expression these plasma samples tested through qRT-PCR....
Accumulating evidence indicates that microRNA-27a (miR-27a) is involved in carcinogenesis and tumor progression. However, the exact function molecular mechanism of miR-27a gastric cancer remain unclear. Quantitative real-time PCR (qRT-PCR) was used to quantify expression its target gene. The investigated through vitro vivo assays (MTT assay, colony formation flow cytometry wound healing migration invasion immunohistochemistry (IHC), immunofluorescence (IF) Western blot). A luciferase...
Abstract Breast cancer (BC) is the most commonly diagnosed malignant tumour in females worldwide. Although remarkable advances early detection and treatment strategies have led to decreased mortality, recurrence metastasis remain major causes of death BC patients. Increasing evidence has demonstrated that circular RNAs (circRNAs) play critical roles progression. However, detailed biological functions molecular mechanisms circRNAs are unclear. The aim this study was investigate possible role...
Abstract MicroRNA‐142‐3p (miR‐142‐3p) was previously investigated in various cancers, whereas, it's role breast cancer (BC) remains far from understood. In this study, we found that miR‐142‐3p markedly decreased both cell lines and BC tumor tissues. Elevated expression suppressed growth metastasis of via gain‐of‐function assay vitro vivo. Mechanistically, could regulate the ras‐related C3 botulinum toxin substrate 1 (RAC1) protein level, which simultaneously epithelial‐to‐mesenchymal...
Recurrent copy-number alterations, mutations, and transcript fusions of the genes encoding CDKs/cyclins are characterized in >10,000 tumors. Genomic alterations dominantly driven by copy number aberrations. In contrast to cell-cycle-related CDKs/cyclins, which globally amplified, transcriptional recurrently lose numbers across cancers. Although mutations relatively rare events, CDK12 exhibits recurrent multiple Among CDKs, CDK7 show most significant loss mutation, respectively. Their genomic...