Abstract Mounting evidences indicate that circular RNAs (circRNAs) have a vital role in human diseases, especially cancers. More recently, circHIPK3, particularly abundant circRNA, was proposed to be involved tumorigenesis. However, its colorectal cancer (CRC) has not been explored. In this study, we found circHIPK3 significantly upregulated CRC tissues and cell lines, at least part, due c-Myb overexpression positively correlated with metastasis advanced clinical stage. Moreover, Cox...

// Feng Wang 1, * , Hou-Qun Ying 2, Bang-Shun He 1 Yu-Qin Pan Qi-Wen Deng Hui-Ling Sun 3 Jie Chen Xian Liu Shu-Kui Central Laboratory, Nanjing First Hospital, Medical University, Nanjing, Jiangsu, China 2 college, Southeast Department of Life Sciences, Normal These authors have contributed equally to this work Correspondence to: Wang, e-mail: shukwangnj@163.com Keywords: hepatocellular carcinoma, lncRNA, UCA1, miR-216b, FGFR1 Received: November 17, 2014 Accepted: January 26, 2015 Published:...

Recent studies indicate that circular RNA (circRNA) plays a pivotal role in cancer progression. Here, we sought to investigate its breast cancer. CircANKS1B (a circRNA originated from exons 5 8 of the ANKS1B gene, hsa_circ_0007294) was identified by RNA-sequencing and validated qRT-PCR Sanger sequencing. Clinical samples were used evaluate expression circANKS1B associations with clinicopathological features prognosis. Gain- loss-of-function experiments cell lines mouse xenograft models...

Colorectal cancer (CRC) is one of the leading causes tumor-related death worldwide, and its main cause distant metastasis. Methyltransferase-like 14(METTL14), a major RNA N6-adenosine methyltransferase, involved in tumor progression via regulating function. The goal study to uncover biological function molecular mechanism METTL14 CRC.Quantitative real-time PCR (qRT-PCR), western blot immunohistochemical (IHC) assays were employed detect SOX4 CRC cell lines tissues. functions demonstrated...

Mounting evidence demonstrates that long noncoding RNAs (lncRNAs) have critical roles during the initiation and progression of cancers. In this study, we report small nucleolar RNA host gene 1 (SNHG1) is involved in colorectal cancer progression.We analyzed sequencing data to explore abnormally expressed lncRNAs cancer. The effects SNHG1 on were investigated through vitro vivo assays (i.e., CCK-8 assay, colony formation flow cytometry EdU xenograft model, immunohistochemistry, western blot)....

Emerging studies suggest that long non-coding RNAs (lncRNAs) play crucial roles in colorectal cancer (CRC). Here, we report a lncRNA, SATB2-AS1, which is specifically expressed tissue and significantly reduced CRC. We systematically elucidated its functions possible molecular mechanisms LncRNA expression CRC was analyzed by RNA-sequencing RNA microarrays. The level of SATB2-AS1 tissues determined quantitative reverse transcription polymerase chain reaction (qRT-PCR) situ hybridization (ISH)....

Background: Exosomal circular RNAs (circRNAs) in peripheral blood are considered as emerging diagnostic biomarkers of cancers. Owing to the lack sensitive and specific biomarkers, a large number colorectal cancer (CRC) patients were diagnosed advanced stages leading high mortality. This study aimed identify circulating exosomal circRNAs novel CRC. Materials Methods: Candidate circRNA was selected by integrating analysis Gene Expression Omnibus (GEO) database with online program GEO2R. A...

Abnormal expression of long non-coding RNAs (lncRNAs) has been found in almost all human tumors, providing numerous potential diagnostic biomarkers, prognostic and therapeutic targets.

Increasing long non-coding RNAs (lncRNAs) have been reported to play key roles in the development and progression of various malignancies. ZNFX1 antisense RNA1 (ZFAS1) has be aberrant expression suggested as a tumor suppressor or oncogene many cancers. However, biological role underlying molecular mechanism ZFAS1, especially miRNA sponge which CRC remain largely unknown. We found that ZFAS1 was higher tissues, where it associated with poor overall survival (OS), we also showed upregulation...

Inflammation plays an integral role in carcinogenesis and tumor progression. Inflammatory response biomarkers have shown to be promising prognostic factors for improving the predictive accuracy various cancers. The aim of this study is investigate significance pre-operative neutrophil lymphocyte ratio (NLR), derived (dNLR), platelet (PLR) monocyte (LMR) gastric cancer (GC).389 patients who had undergone gastrectomy were enrolled from 2007 2009 study. NLR, dNLR, PLR LMR calculated peripheral...

Epigenetic alterations contributed to human carcinogenesis immensely. N6-methyladenosine (m6A) is one of the most preventive and abundant modifications on RNA molecules present in eukaryotes. However, biological function m6A methylation colorectal cancer (CRC) remains largely unclear. Here, we found that METTL14 was downregulated CRC tissues cell lines, closely correlated with overall survival (OS). knockdown significantly reduced levels total RNAs promoted growth metastasis, whereas...

ABSTRACT Colorectal cancer (CRC) is one of the most common malignancies. Hypoxia can promote occurrence and development CRC. However, how hypoxia regulates CRC immune microenvironment needs to be further explored. The bulk RNA sequencing data clinicopathological information patients were enrolled from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases. single‐cell (scRNA‐seq) datasets collected analyzed GEO database ArrayExpress database. score gene set was estimated using...

Inflammation is an essential component of pathogenesis and progression cancer. A high neutrophil-to-lymphocyte ratio (NLR) considered as a prognostic indicator for breast This meta-analysis was conducted to establish the overall accuracy NLR test in diagnosis comprehensive search literature by using PubMed, Web Science China National Knowledge Infrastructure (CNKI). Published studies dating up July 2014 4,293 patients were enrolled present study. In order evaluate association between...

Background: Hepatocellular carcinoma (HCC) is one of the most aggressive cancers, with limited new diagnostic and therapeutic measures.This study aimed to investigate utility specific serum exosome lncRNAs as biomarkers for early diagnosis HCC.Methods: The relative expression levels eight selected in were evaluated by quantitative reverse transcription-PCR (qRT-PCR) training validation sets HCC patients matched healthy controls.Additionally, stability, specificity efficiency these determine...

MicroRNA-150-5p (miR-150-5p) has been implicated in tumor initiation and progression a variety of cancers. However, its roles colorectal cancer (CRC) remain largely unknown. In our study, decreased miR-150-5p expression CRC tissues was found to be associated with poor overall survival. Moreover, inhibited cell proliferation, migration, invasion angiogenesis vitro vivo, inhibitory effect could reversed by transfection vascular epithelial growth factor A (VEGFA) plasmid. Lastly, we...

Abstract MicroRNAs (miRNAs) are important class of functional regulators involved in human cancers development, including colorectal cancer (CRC). Exploring aberrantly expressed miRNAs may provide us with new insights into the initiation and development CRC by functioning as oncogenes or tumor suppressors. The aim our study is to discover expression pattern miR-1249 investigate its clinical significance well biological role progression. In study, we found that was markedly downregulated...

Clinically, one of the principal factors in failure advanced colorectal cancer (CRC) treatment is chemoresistance to 5-fluorouracil (5FU)-based chemotherapy. Although microRNA-375-3p (miR-375) considered a tumor suppressor multiple cancers, mechanism miR-375 regulation drug resistance CRC remains unclear. In this study, we investigated chemosensitivity 5FU from biological and clinical aspects. We found that was significantly downregulated tissues cell lines, low expression strongly...

Introduction This study investigates the potential of Bifidobacterium breve BB05 (BB05) in mitigating depressive symptoms a mouse model Chronic Unpredictable Mild Stress (CUMS), with fluoxetine as positive control. Methods and Results High-dose (1.0 × 10 9 CFU/kg, BB05H) significantly reduced anxiety- depression-like behaviors CUMS mice, measured by open field test, tail suspension forced swim test. treatment also pathological ileal damage, alleviated inflammation, lowered serum levels...

Abstract As one of the most common cancers worldwide, colorectal cancer (CRC) causes a large number mortality annually. Aberrant expression microRNAs (miRNAs) is significantly associated with initiation and development CRC. Further investigations regarding regulatory mechanism miRNAs warranted. In this study, we discovered that miR-486-5p was remarkably downregulated in CRC, which partially results from higher DNA methylation promoter region detected by using methylation-specific PCR,...

Background Long noncoding RNAs (lncRNAs) play widespread roles in gene regulation and cellular processes. However, the functional of lncRNAs colorectal cancer (CRC) are not yet well elucidated. The aim present study was to measure levels lncRNA 91H expression CRC evaluate its clinical significance biological development progression CRC. Methods copy number variation (CNV) were measured 72 tumor tissues adjacent normal by real-time PCR. evaluated MTT, scratch wound assay, migration invasion...