Abstract Background Study on the expression of miRNA‐22 in serum Alzheimer's disease (AD) patients and mechanism neuroinflammation regulation. Methods ELISA assay was used to detect level inflammatory factors, including interleukin‐1β (IL‐1β), interleukin‐18 (IL‐18), tumor necrosis factor‐α AD patients. TargetScan database luciferase reporter gene indicated that gasdermin D (GSDMD) target miRNA‐22. mimic transfected into microglia, followed by administration LPS Nigericin induce pyroptosis....

Takayasu arteritis (TAK) is an inflammatory vasculitis that affects the aorta and its primary branches. The pathogenesis of TAK remains elusive, yet identifying key cell types in patients crucial for uncovering cellular heterogeneity discovering potential therapeutic targets. This study utilized single-cell transcriptome analysis on aortic specimens from three patients, with control data sourced a publicly available database (GSE155468). Additionally, bulk RNA sequencing was performed...

Abstract Objective To detect the expression of high‐mobility group box protein 1 (HMGB1) and toll‐like receptor 4 (TLR4) their downstream signaling factors—myeloid differentiation factor 88 (MyD88), nuclear kappa B (NF‐κB), tumor necrosis alpha (TNF‐α)—in sera patients with Parkinson's disease (PD) in order to evaluate relationship HMGB1–TLR4 axis PD development progression. Methods The serum HMGB1 TLR4 levels 120 100 healthy volunteers were measured using double‐antibody sandwich ELISA,...

Coronaviruses are a family of large positive-sense RNA viruses that responsible for wide range important veterinary and human diseases. Nsp1 has been shown to have an role in the pathogenetic mechanisms coronaviruses vivo. To assess function relatively conserved domain (LLRKxGxKG) MHV nsp1, mutant virus, MHV-nsp1-27D, with 27 nts deletion was constructed using reverse genetic system vaccinia virus vector. The had similar growth kinetics MHV-A59 wild-type 17CI-1 cells, but highly attenuated...

Highly active anti-retroviral therapy (HAART) is very effective in suppressing HIV-1 replication patients. However, continuous HAART required to prevent viral rebound, which may have detrimental effects various tissues, including persistent neuroinflammation the central nervous system (CNS). Here, we show that quercetin (3,5,7,3',4'-pentahydroxy flavones), a natural antioxidant used Chinese traditional medicines, suppresses induced by chronic exposure Zidovudine (azidothymidine, AZT),...

Parkinson's disease (PD) is a common degenerative of the central nervous system (CNS). The main pathological change apoptosis dopaminergic neurons in substantia nigra pars compacta (SNPc), thereby leading to dopamine reduction nigral striatum. 6-Hydroxydopamine (6-OHDA), neurotoxic substance, mediates and causes Parkinson-like symptoms mice.

Endoplasmic reticulum (ER) perturbations are novel subcellular effectors involved in the ischaemia-reperfusion injury. As an ER stress-inducible protein, mesencephalic astrocyte-derived neurotrophic factor (MANF) has been proven to be increased during ischaemic brain However, role of MANF liver ischaemia reperfusion (I/R) injury not yet studied.To investigate process ischaemia-reperfusion, Hepatocyte-specific knockout (MANFhep-/- ) mice and their wild-type (WT) littermates were used our...

Abstract In this study, FoxO1 transgenic mice (transgenic, FoxO1‐Tg) and C57BL/6 wild‐type (wild‐type, FoxO1‐WT) were used to establish chronic colitis by drinking water containing dextran sulphate sodium (DSS). Afterwards, we observed the life changes in assessed pathological H&E tissue staining. addition, TLR4/MyD88/MD2‐NF‐κB inflammatory signals detected. As a result, under DSS treatment, activation level of signal was higher FoxO1‐Tg than that FoxO1‐WT mice. Meanwhile, intestinal...

Abstract This study aimed to investigate the role of protein kinase HIPK2 in depression and its associated mechanism. The chronic unpredictable mild stress (CUSM) model was constructed simulate mice with detect mouse behaviors. Moreover, by using microglial cells BV2 as model. After conditional knockdown HIPK2, depressive behavior disorder improved, meanwhile, neuroinflammation alleviated, M1 cell proportion reduced. Similar results were obtained after applying inhibitor tBID or ASO‐HIPK2...

Background: Traditional Chinese Medicine categorizes melasma into various syndromes, one of which is the kidney-deficiency and blood-stasis syndrome. Different medicines are used for each syndrome to achieve a personalized treatment approach, resulting in more effective outcome. The study aimed investigate efficacy safety Lutai Danshen Baishao Granules (LDBG) alleviating compare effects LDBG patients with without through real-world, large-sample investigation. Methods: A multicenter,...

Atrial fibrillation (AF) is a common arrhythmia in clinical. Its most important pathophysiological factor atrial fibrosis. Transferrin receptor (TFRC) promotes ferroptosis by facilitating iron uptake. role AF unknown. TFRC expression Angiotensin II (Ang II)-induced mice was significantly upregulated. knockdown reduced occurrence. silence ameliorated myocardial fibrosis inhibiting TGF-β1/Smad2 pathway vivo. interference lipid oxidation product generation Ang II-induced HL-1 cardiomyocyte...

The interplay between green innovation and corporate value is critical in contemporary business research, due to environmental challenges market shifts toward sustainability. A review of 10 years studies across industries identifies three dimensions: innovation's dual role, ESG integration, regional/stakeholder factors. Key findings show enhances competitive advantage, but overinvestment risks exist. Hybrid portfolios balance creation. reconciles Porter Hypothesis with resource-based...

Insufficient physical activity among adolescents is a major global public health concern, and schools are considered key venues for promoting activity. Teachers play crucial role in the implementation of policies. This study adapted validated COM-PASS scale, based on COM-B model, to assess its reliability validity Chinese context. validates appropriateness scale assessing within cultural A three-phase design was employed: (1) Delphi method involving three rounds surveys with 15 experts...

Parkinson's disease (PD) is a neurodegenerative disease, and the role of neuroinflammation in pathogenesis progression PD has been confirmed. The polysaccharides triterpenoids antrodia camphorata (a polyporous fungus) harbor diverse powerful pharmacological effects. In this study, 6-hydroxydopamine was used to construct mouse model. After polysaccharide (ACP) intervention, neurobehavioral changes were detected, neurotransmitter striatum determined by high-performance liquid chromatography,...

Abstract We mainly study the role and regulatory mechanism of double‐negative T cells (DNTs) in Alzheimer's disease (AD). The mice splenic DNTs were separated amplified by Rosettesep antibody adsorption method Easysep magnetic activated cell sorting. intraperitoneally injected into APP/PS1‐AD model, which was found to aggravate cognitive impairment mice. secreted tumor necrosis factor α (TNF‐α) promote activation NLRP3 M1 polarization microglial cells, silencing with small interfering RNA...

This study illustrated the liver protection mechanism of ACP from perspective autophagy activation. suppressed inflammatory injury KCs, and decreased cell apoptosis rate. After LTG LC3 staining, promoted lysosomal production, increased expression, activated autophagy, expression NLRP3 factors. Under electron microscope, accelerated production autophagosomes. simultaneous treatment with 3-MA ACP, effect on resisting KC decreased, was up-regulated, suppressed. As discovered in mouse model...

Abstract Background Trastuzumab, a monoclonal antibody for breast cancer, faces global accessibility challenges, primarily due to high costs. This study examines how changes in medical insurance policies and price adjustments influence Trastuzumab utilization China, focusing on implementation challenges their impact drug affordability. Methods retrospective investigated among HER2-positive cancer patients at tertiary hospital from May 2013 December 2022 JX, prefecture-level city eastern...

This study aimed to investigate the mechanism in regulating cross talk between glomerular endothelial cells and podocytes "occupational medicamentosa-like dermatitis induced by trichloroethylene (OMLDT)" patients.Totally 6 OMLDT patients, 18 controls, 102 BALB/c female mice were involved this study. Patient's serum endothelin-1 (ET-1), angiopoietin-1 (Ang-1) angiopoietin-2 (Ang-2), blood urea nitrogen (BUN), podocalyxin (PCX) detected. All used establish (TCE) sensitized mouse model....

Background: Glaucocalyxin B (Gla B) is a type of sesquiterpenoids. At present, there are rare studies on the pharmacological effects and targets sesquiterpenoids, while multiple sesquiterpenoids have good anti-inflammatory properties. Therefore, in this study, we aimed to investigate mechanism Gla macrophages rheumatoid arthritis. Methods: LPS/IFN-γ was used induce M1 polarization synovial macrophage (SMG) vitro, followed by pretreatment (5 μM 15 μM). Afterwards, flow cytometry performed...

Outbreaks of short beak dwarf syndrome caused by novel goose parvovirus (NGPV) have been prevalent in China since 2015, resulting a high mortality rate ducks. Herein we evaluated differences between two NGPV strains: Muscovy duck-origin (AH190917-RP: MD17) and Cherry Valley (JS191021-RP: CVD21) NGPV. Both them showed certain level pathogenicity to primary duck embryo fibroblasts, embryos ducklings. CVD21 comparatively stronger than MD17. Only obvious cytopathic effect (CPE), characterized...

Our previous study has found that aureusidin can inhibit inflammation by targeting myeloid differentiation 2 (MD2) protein. Structural optimization of gave rise to a derivative named CNQX. LPS was used induce in intestinal macrophages; flow cytometry, PI staining and Hoechst 33342 were detect the apoptotic level enzyme-linked immunosorbent assay (ELISA) utilized expression inflammatory factors (including IL-1β, IL-18 TNF-α); immunofluorescence investigate MD2; Western blot employed measure...

Inflammatory bowel disease (IBD) is a chronic nonspecific inflammatory with an unclear etiology. The active ingredients of traditional Chinese medicines (TCMs) exert anti-inflammatory, antitumor, and immunomodulatory effects, their multitarget characteristics provide them unique advantage for treating IBD. However, the therapeutic effects underlying mechanisms Xi Lei San in treatment IBD remain unknown. This study was designed to investigate whether exerted anti-inflammatory effect via...