A5081706870- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Supramolecular Self-Assembly in Materials
- Virus-based gene therapy research
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Bacteriophages and microbial interactions
- Animal Disease Management and Epidemiology
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- Plant Virus Research Studies
- vaccines and immunoinformatics approaches
- Herpesvirus Infections and Treatments
- Plant Gene Expression Analysis
- Tryptophan and brain disorders
- Parasitic infections in humans and animals
- Quantum Dots Synthesis And Properties
- Protein Structure and Dynamics
Chinese Academy of Agricultural Sciences
2020-2025
Agricultural Genomics Institute at Shenzhen
2025
Ministry of Agriculture and Rural Affairs
2024-2025
Jilin Academy of Agricultural Sciences
2022-2025
Nanyang Technological University
2019-2025
Harbin Veterinary Research Institute
2022-2025
Qingdao Agricultural University
2022-2024
Sichuan Normal University
2024
Collaborative Innovation Center of Chemistry for Energy Materials
2023-2024
Jiangsu University of Science and Technology
2024
Vaccine-induced cellular immunity controls virus replication in simian immunodeficiency (SIV)-infected monkeys only transiently, leading to the question of whether such vaccines for AIDS will be effective. We immunized with plasmid DNA and replication-defective adenoviral vectors encoding SIV proteins then challenged them pathogenic SIV. Although these demonstrated a reduction viremia restricted early phase infection, they showed prolonged survival. This survival was associated preserved...
Liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs) is involved in both intracellular membraneless organelles and extracellular tissues. Despite growing understanding LLPS, molecular-level mechanisms behind this process are still not fully established. Here, we use histidine-rich squid beak (HBPs) as model IDPs to shed light on molecular interactions governing LLPS. We show that LLPS HBPs mediated though specific modular repeats. The morphology separated phases...
Abstract Coacervation, or liquid-liquid phase separation (LLPS) of biomacromolecules, is increasingly recognized to play an important role both intracellularly and in the extracellular space. Central questions that remain be addressed are links between material properties coacervates (condensates) primary secondary structures their constitutive building blocks. Short LLPS-prone peptides, such as GY23 variants explored this study, ideal model systems investigate these because simple sequence...
A vaccine protecting monkeys against mucosal infection by simian immunodeficiency virus sheds light on immune and genetic correlates of protection.
CRISPR/Cas9-based genome editing tools have enormous potential for the development of various therapeutic treatments due to their reliability and broad applicability. A central requirement CRISPR/Cas9 is efficient intracellular delivery machinery, which remains a well-recognized challenge, notably deliver Cas9 in its native protein form. Herein, phase-separating peptide with redox-triggered release properties employed encapsulate all three forms CRISRP-Cas9 namely, pDNA, mRNA/sgRNA,...
Bats are associated with the circulation of most mammalian filoviruses (FiVs), pathogenic ones frequently causing deadly hemorrhagic fevers in Africa. Divergent FiVs have been uncovered Chinese bats, raising concerns about their threat to public health. Here, we describe a long-term surveillance track bat at orchards, eventually resulting identification and isolation FiV, Dehong virus (DEHV), from Rousettus leschenaultii bats. DEHV has typical filovirus-like morphology wide spectrum cell...
Peptide coacervates self-assembling via liquid-liquid phase separation are appealing intracellular delivery vehicles of macromolecular therapeutics (proteins, DNA, mRNA) owing to their non-cytotoxicity, high encapsulation capacity, and efficient cellular uptake. However, the mechanisms by which these viscoelastic droplets cross membranes remain unknown. Here, using multimodal imaging, data analytics, biochemical inhibition assays, identify key steps enter cell. find that uptake follows a...
Abstract Expansion of two monocyte subpopulations expressing CD16 and their correlation with viral load are observed in this cross-sectional study SIV-infected macaques. Infection by HIV is associated the expansion monocytes antigens, but significance pathogenesis largely unknown. In rhesus macaques, at least three blood were identified based on expression CD14 CD16: CD14highCD16−, CD14highCD16low, CD14lowCD16high. The phenotypes functions these subpopulations, including CD16+ monocytes,...
Porcine reproductive and respiratory syndrome viruses (PRRSVs) are significant pathogens that affect the global swine industry. Its virions consist of a central core composed nucleocapsid (N) protein, surrounded by multiple distinct viral envelope proteins. However, mechanisms underlying recognition packaging N protein proteins remain elusive. In this study, we elucidated role nonstructural 2 (nsp2) from highly pathogenic PRRSV-2 (HP-PRRSV-2) in assembly. Firstly, among all tested proteins,...
Gloriosa L. possesses exceptional ornamental value, with its floral hues exhibiting a wide range of variations. In this study, we employed sophisticated colorimetry, Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS), and transcriptome sequencing to investigate the phenotypic expression tepal colors, composition carotenoids anthocyanins, differential gene in four varieties during their full bloom phase. Our findings revealed that redness tepals, indicated by higher...
With the recent demonstration in RV144 Thai trial that a vaccine regimen does not elicit neutralizing antibodies or cytotoxic T lymphocytes may confer protection against human immunodeficiency virus type 1 (HIV-1) infection, attention has turned to nonneutralizing as possible mechanism of protection. In current study, we evaluated kinetics antibody-dependent cell-mediated cytotoxicity (ADCC) response during acute and chronic SIVmac251 infection rhesus monkeys. We first adapted flow...
Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases affecting pig-raising industry. The PRRS virus (PRRSV) has high genetic diversity, partly owing to viral recombination. Some individual recombinant type 2 PRRSV (PRRSV-2) strains have been detected; however, sequence composition characteristics recombination hot spots potential driving forces for PRRSV-2 are still unreported. Therefore, all available genomic sequences (n = 949, including...
ABSTRACT Antibody-dependent cell-mediated viral inhibition (ADCVI) is an attractive target for vaccination because it takes advantage of both the anamnestic properties adaptive immune response and rapid early characteristics innate response. Effective utilization ADCVI in vaccine strategies will depend on understanding natural history during acute chronic human immunodeficiency virus type 1 (HIV-1) infection. We used simian (SIV)-infected rhesus monkey as a model to study kinetics infection,...
Binding of antibodies to their receptors is a core component the innate immune system. Understanding precise interactions between and Fc has led engineering novel mAb biotherapeutics with tailored biological activities. One most significant findings that afucosylated monoclonal demonstrate increased affinity toward receptor FcγRIIIa, commensurate increase in antibody-dependent cellular cytotoxicity. Crystal structure analysis hypothesis afucosylation region results reduced steric hindrance...
Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is considered one of most devastating swine diseases worldwide. PRRSV-1 was first isolated in China 2006. However, there were few reports concerning genetic characteristics China. In this study, three strains (HL85, HeB3, HeB47) detected a general RT-qPCR method from clinical samples 2018. HeB47 identified as recombinant between BJEU06-1 CReSA228-like strains. To further analyze recombination deletion...
Abstract Background The global pork industry is continuously affected by infectious diseases that can result in large-scale mortality, trade restrictions, and major reductions production. Nevertheless, the cause of many pigs remains unclear, largely because commonly used diagnostic tools fail to capture full diversity potential pathogens pathogen co-infection common. Results We a meta-transcriptomic approach systematically characterize 136 clinical cases representing different disease...
ABSTRACT Understanding the characteristics of virus-specific T-lymphocyte response that will confer optimal protection against clinical progression AIDS inform development an effective cellular immunity-based human immunodeficiency virus vaccine. We have recently shown survival in plasmid DNA-primed/recombinant adenovirus-boosted rhesus monkeys are challenged with simian SIVmac251 is associated preservation postchallenge central memory CD4 + T lymphocytes and robust gamma interferon...
Viral metagenomics is the most powerful tool to profile viromic composition for a given sample. Different methods, including amplification-free ones, have been developed, but choosing them different purposes requires comprehensive benchmarks. Here, we assessed performance of four routinely used i.e., multiple displacement amplification (MDA), direct metagenomic sequencing (MTG), sequence-independent single-primer (SIA), and metatranscriptomic (MTT), using marmot rectal samples as templates...
While mycobacteria have been proposed as vaccine vectors because of their persistence and safety, little has done systematically to optimize immunogenicity in nonhuman primates. We successfully generated recombinant Mycobacterium bovis BCG (rBCG) expressing simian immunodeficiency virus (SIV) Gag Pol multigenic, nonintegrating vectors, but rBCG-expressing SIV Env was unstable. A dose route determination study rhesus monkeys revealed that intramuscular administration rBCG associated with...