A5026177743- Neuroscience of respiration and sleep
- Spinal Cord Injury Research
- Respiratory Support and Mechanisms
- Lysosomal Storage Disorders Research
- Sleep and Wakefulness Research
- Neurogenetic and Muscular Disorders Research
- Neonatal Respiratory Health Research
- Glycogen Storage Diseases and Myoclonus
- Neuroendocrine regulation and behavior
- Congenital Diaphragmatic Hernia Studies
- Infant Health and Development
- Child Nutrition and Feeding Issues
- Heart Rate Variability and Autonomic Control
- Neonatal and fetal brain pathology
- Obstructive Sleep Apnea Research
- High Altitude and Hypoxia
- Spinal Dysraphism and Malformations
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cardiac Arrest and Resuscitation
- Urinary Bladder and Prostate Research
- Urological Disorders and Treatments
- Virus-based gene therapy research
- Genetics and Neurodevelopmental Disorders
- Infant Development and Preterm Care
- Nerve Injury and Rehabilitation
Allen Institute for Brain Science
2011-2025
University of Florida
2016-2025
Institute for Breathing and Sleep
2023-2024
Glenn Research Center
2024
Breathe California of Sacramento Emigrant Trails
2023
Florida Center for Gastroenterology
2016-2019
American Physical Therapy Association
2008-2017
Florida College
2008-2017
University of Wisconsin–Madison
2000-2011
Diocese of Brooklyn
2007
We tested the hypothesis that chronic intermittent hypoxia (CIH) elicits plasticity in central neural control of breathing via serotonin-dependent effects on integration carotid chemoafferent inputs. Adult rats were exposed to 1 week nocturnal CIH (11-12% O(2)/air at 5 min intervals; 12 hr/night). and untreated then anesthetized, paralyzed, vagotomized, artificially ventilated. Time-dependent hypoxic responses assessed phrenic neurogram during after three episodes isocapnic hypoxia....
1 The purpose of these experiments was to examine the mechanisms by which either co-activation or independent activation tongue protrudor and retractor muscles influence upper airway flow mechanics. We studied selective hypoglossal (XIIth) nerve stimulation on movements mechanics in anaesthetized rats that were prepared with an isolated airway. In this preparation, both nasal oral pathways are available. 2 Inspiratory limitation achieved rapidly lowering hypopharyngeal pressure (Php) a...
Episodic hypoxia evokes a sustained augmentation of respiratory motor output known as long-term facilitation (LTF). Phrenic LTF is prevented by pretreatment with the 5-hydroxytryptamine (5-HT) receptor antagonist ketanserin. We tested hypothesis that 5-HT activation necessary for induction but not maintenance phrenic LTF. Peak integrated nerve activity (∫Phr) was monitored 1 h after three 5-min episodes isocapnic (arterial Po 2 = 40 ± Torr; hyperoxic intervals) in four groups anesthetized,...
Pompe disease is a severe form of muscular dystrophy due to glycogen accumulation in all tissues, especially striated muscle. Disease severity directly related the deficiency acid α-glucosidase (GAA), which degrades lysosome. Respiratory dysfunction hallmark disease, muscle weakness has been viewed as underlying cause, and possibility an associated neural contribution not evaluated previously. Therefore, we examined behavioral neurophysiological aspects breathing 2 animal models disease—the...
Abstract Although monosynaptic bulbospinal projections to phrenic motoneurons have been extensively described, little is known about the organization of premotor neurons in adult rat spinal cord. Because interneurons may play an important role normal breathing and recovery following cord injury, present study has used anterograde transneuronal retrograde tracing their distribution synaptic relations. Exclusive unilateral, first‐order labeling motoneuron pool with pseudorabies virus...
Pompe disease is an inherited neuromuscular caused by deficiency of lysosomal acid alpha-glucosidase (GAA) leading to glycogen accumulation in muscle and motoneurons. Cardiopulmonary failure infancy leads early mortality, GAA enzyme replacement therapy (ERT) results improved survival, reduction cardiac hypertrophy, developmental gains. However, many children have progressive ventilatory insufficiency need additional support. Preclinical work shows that gene transfer restores phrenic neural...
Rationale: Intermittent stimulation of the respiratory system with hypoxia causes persistent increases in motor output (i.e., long-term facilitation) animals spinal cord injury. This paradigm, therefore, has been touted as a potential rehabilitation strategy.Objectives: To determine whether acute (daily) exposure to intermittent can also evoke facilitation ventilation after chronic injury humans, and repeated daily enhances magnitude this response.Methods: Eight individuals incomplete (>1...
A first-in-human trial of diaphragmatic gene therapy (AAV1-CMV-GAA) to treat respiratory and neural dysfunction in early-onset Pompe disease was conducted. The primary objective this study assess the safety rAAV1-CMV-hGAA vector delivered diaphragm muscle subjects with ventilatory insufficiency. Safety assessed by measurement change serum chemistries hematology, urinalysis, immune response GAA AAV, as well level health. data demonstrate that AAV treatment safe there were no adverse events...
Abstract Recent work shows that certain antibody-based assays for the neurofilament light chain detect informative signals in CSF and blood of human animals affected by a variety CNS injury disease states. Much this has been performed using two mouse monoclonal antibodies to light, UD1 UD2, also known as Clones 2.1 47.3, respectively. These are essential components Uman Diagnostics Neurofilament-Light™ ELISA kit, Quanterix Simoa™ bead-based assay others. We show both bind neighbouring...
Spinal hemisection at C2 reveals caudal synaptic pathways that cross the spinal midline (crossed phrenic pathways) and can restore inspiratory activity in ipsilateral motoneurons. Intermittent hypoxia induces plasticity cervical cord, resulting enhanced motor output. We hypothesized chronic intermittent (CIH) (alternating 11% O(2) air; 5 min periods; 12 hr per night; 7 nights) would strengthen crossed pathways. Experiments were performed on anesthetized, vagotomized, paralyzed, ventilated,...
We tested the hypothesis that unanesthetized rats exhibit ventilatory long-term facilitation (LTF) after intermittent, but not continuous, hypoxia. Minute ventilation (V˙e) and carbon dioxide production (V˙co 2 ) were measured in unanesthetized, unrestrained male Sprague-Dawley via barometric plethysmography before, during, exposure to continuous or intermittent Hypoxia was either isocapnic [inspired O fraction (Fi = 0.08–0.09 inspired CO 0.04] poikilocapnic 0.11 Fi 0.00). Sixty minutes...
Our primary purpose was to test the hypothesis that tongue protrudor (genioglossus, GG) and retractor (styloglossus, SG hyoglossus, HG) muscles are co‐activated when respiratory drive increases, co‐activation will cause retraction of tongue. This addressed by performing two series experiments using a supine, anaesthetized, tracheotomized rat in which muscle force neural could be measured during spontaneous breathing. In first experiments, increased progressively occluding tracheal cannula...
We tested two hypotheses: 1) that the spontaneous enhancement of phrenic motor output below a C2 spinal hemisection (C2HS) is associated with plasticity in ventrolateral inputs to motoneurons; and 2) recovery anesthetized rats after C2HS correlates increased capacity generate inspiratory volume during hypercapnia unanesthetized rats. At 2 4 wk post-C2HS, ipsilateral nerve activity was recorded anesthetized, paralyzed, vagotomized, ventilated Electrical stimulation funiculus contralateral...