A5056751083- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Plasma Applications and Diagnostics
- Glycosylation and Glycoproteins Research
- Thermoregulation and physiological responses
- Skin Protection and Aging
- Dermatologic Treatments and Research
- melanin and skin pigmentation
- Cellular Mechanics and Interactions
- Textile materials and evaluations
- 3D Printing in Biomedical Research
- Chemical Synthesis and Analysis
- Cell Adhesion Molecules Research
- Cancer, Lipids, and Metabolism
- Nitric Oxide and Endothelin Effects
- Lymphatic System and Diseases
- Cancer therapeutics and mechanisms
- Neurobiology and Insect Physiology Research
- Galectins and Cancer Biology
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Exercise and Physiological Responses
- Vector-Borne Animal Diseases
- Electron Spin Resonance Studies
- Mathematical Biology Tumor Growth
Centre de Biophysique Moléculaire
2015-2024
Centre National de la Recherche Scientifique
2012-2023
Université d'Orléans
1991-2020
Inserm
2017-2020
Jagiellonian University
2013
Le Studium
2013
Institut de Chimie des Substances Naturelles
1990-2009
Institut Gustave Roussy
1998
Tumour microenvironment determines the fate of treatments. Reconstitution tumour conditions is mandatory for alternative in vitro methods devoted to cancer development and selection therapeutic strategies. This work describes a 3D model melanoma growth its environment. Introducing means mimic angiogenesis, which turns on progression, shows that spheroids allow reconstitution solid tumours with stromal cells. Angiogenesis evidenced differential recruitment endothelial cells (EC) from early...
Abstract Several attempts have been made to deliver reactive oxygen and nitrogen species (RONS) produced by non-thermal plasma onto large surfaces in a controllable way compatible with skin treatments. In this paper, the effect of pulse frequency, range 0.5–20 kHz, on discharge behavior was studied tissue models evaluate potential treatment changes. This has done through electrical characterization, visualization helium flow (by Schlieren technique), jet modifications (ICCD imaging) RONS...
It is shown that AcSDKP a new regulator of the hematopoietic system can he generated from thymosin β 4 by one‐step enzymatic cleavage in vitro and vivo. Tβ were both detected bone marrow cells (BMC'). Incubation [ 3 H]Tβ with either intact or lysed BMC led to formation H]AcSDKP whereas labelled tetrapeptide was not degraded under these conditions. Model degradation carried out bacterial enzymes suggests mammalian endoproteinase Asp‐N might be involved through specific Pro‐ 5 Asp peptide bond T .
Tumor hypoxia is a characteristic of cancer cell growth and invasion, promoting angiogenesis, which facilitates metastasis. Oxygen delivery remains impaired because tumor vessels are anarchic leaky, contributing to dissemination. Counteracting by normalizing in order improve drug radio therapy efficacy avoid stem-like selection highly challenging issue. We show here that inositol trispyrophosphate (ITPP) treatment stably increases oxygen tension blood flow melanoma breast syngeneic models....
Abstract VEGFs are found at high levels in hypoxic tumors. As major components directing pathologic neovascularization, they regulate stromal reactions. Consequently, novel strategies targeting and inhibiting VEGF overproduction upon hypoxia offer considerable potential for modern anticancer therapies controlling rather than destroying tumor angiogenesis. Here, we report the design of a vector expressing soluble form receptor-2 (sVEGFR2) driven by hypoxia-responsive element (HRE)-regulated...
Abstract Endothelial progenitor cells (EPCs) modulate postnatal vascularization and contribute to vessel regeneration in adults. Stem were found umbilical cord blood, bone marrow, mobilized peripheral blood cells, from where they isolated cultured. However, the yield of is usually not sufficient for clinical application quality varies. The aim study was immortalization early with high proliferative potential, capable differentiate EPCs and, further, toward endothelial cells. Two cell lines,...
Heparanase is overexpressed by tumor cells and degrades the extracellular matrix proteoglycans through cleavage of heparan sulfates (HS), allowing pro-angiogenic factor release thus playing a key role in angiogenesis metastasis. Here we propose new HS analogs as potent heparanase inhibitors: Heparin positive control, Dextran Sulfate, λ-Carrageenan, modified forms them obtained depolymerization associated to glycol splitting (RD-GS). After activity assessment, 11 kDa RD-GS-λ-Carrageenan...
Podoplanin (PDPN), an O-glycosylated, transmembrane, mucin-type glycoprotein, is expressed by cancer associated fibroblasts (CAFs). In malignant transformation, PDPN subjected to changes and its role yet be established. Here we show that it involved in modulating the activity of CCL21/CCR7 chemokine/receptor axis a hypoxia-dependent manner. present model, breast MDA-MB-231 cells NKL3 express surface CCR7 receptor for CCL21 chemokine which potent chemoattractant able bind PDPN. The impact...
Abstract Pathologic angiogenesis directly responds to tumour hypoxia and controls the molecular/cellular composition of microenvironment, increasing both immune tolerance stromal cooperation with growth. Myo‐inositol‐trispyrophosphate (ITPP) provides a means achieve stable normalization angiogenesis. ITPP increases intratumour oxygen tension (pO 2 ) stabilizes vessel through activation endothelial Phosphatase‐and‐Tensin‐homologue (PTEN). Here, we show that reduction due ITPP‐induced...
Polyphenols are strong antioxidant molecules allowing prevention of skin photo-ageing damages, but their use is limited due to low solubility and toxicity towards cells. We postulated that enzymatic glucosylation could improve solubility, stability and, consequently, efficacy. The aim this work was study changes induced by addition a glucose moiety on two polyphenols displaying very different chemical structures [caffeic acid (CA), epigallocatechin-3-gallate (EGCG) there glucosylated form,...
Hypoxia-inducing pathologies as cancer develop pathologic and inefficient angiogenesis which rules tumor facilitating microenvironment, a key target for therapy. As such, the putative ability of endothelial precursor cells (EPCs) to specifically home hypoxic sites neovascularization prompted design optimized, site-specific, cell-mediated, drug-/gene-targeting approach. Thus, EPC lines were established from aorta-gonad-mesonephros (AGM) murine 10.5 dpc 11.5 embryo when repertoire is...
The biological effects induced by cold atmospheric plasmas (CAPs) on human tissues are mainly due to the production of reactive oxygen and nitrogen species (RONS). Some RONS also responsible for pH lowering treated medium. CAP-induced acidification has beneficial effect tissues, contributing anti-bacterial healing improvement observed in wound. In this work we investigated local a helium CAP treatment using tissue models made agarose gels with adjusted around 7.4 mimic generic organs or 5.5...
Despite the large number of papers showing biological effect cold atmospheric plasmas (CAPs) on skin treatment and although their ability to produce a cocktail reactive oxygen nitrogen species (RONS) is largely known, deep knowledge these ionized gas sources far be uncovered. Many parameters take part in complex mechanisms induced by plasma, such as voltage, frequency, feeding flow rate, temperature, air humidity, composition. In this paper, we focus our attention influence dissolved medium...
Keratinocytes prevent skin photoaging by ensuring the defence against oxidative stress, an excessive production of reactive oxygen species (ROS). They are localized within epidermis where level (1-3% O2), named physioxia, is low compared to other organs. Oxygen essential for life but also generates ROS. Most in vitro studies on keratinocyte antioxidant capacities performed under atmospheric oxygen, normoxia, which very far from physiological microenvironment, thus submitting cells...
Abstract Skin injury leading to chronic wounds is of high interest due the increasing number patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in wound healing processes. For that reason, controlled promotion these processes required. In work, we present portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system gas temperature for biological activities. To make He-DBD-based safe purposes, a multivariate optimization...
The skin constitutes with its microbiota the first line of body defense against exogenous stress including air pollution. Especially in urban or sub-urban areas, it is continuously exposed to many environmental pollutants gaseous nitrogen dioxide (gNO 2 ). Nowadays, well established that pollution has major effects on human skin, inducing various diseases often associated microbial dysbiosis. However, very few known about impact microbiota. In this study, a new approach was adopted, by...
In an attempt to identify cell surface molecules involved in recognition phenomena between cells such as keratinocytes and melanocytes putatively target biological responses modifiers keratinocytes, we undertook the detection of sugar specific receptors: membrane lectins. Keratinocyte lectins were found bind synthetic glycoproteins (neoglycoproteins) carrying either alpha-L-fucosyl or alpha-L-rhamnosyl residues. Fluorescence microscopy observations indicate that cultured are able these two...
Bartonella henselae, a zoonotic agent, induces tumors of endothelial cells (ECs), namely bacillary angiomatosis and peliosis in immunosuppressed humans but not cats. In vitro studies on ECs represent to date the only way explore interactions between henselae vascular endothelium. However, no comparative study human (incidental host) vs feline (reservoir has been carried out because absence any available cell lines. To this purpose, we have developed nine EC lines which allowed comparing...