A5050032465- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Electrospun Nanofibers in Biomedical Applications
- Cell Adhesion Molecules Research
- Pregnancy and preeclampsia studies
- Immunotherapy and Immune Responses
- Drug Transport and Resistance Mechanisms
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- RNA Interference and Gene Delivery
- Psoriasis: Treatment and Pathogenesis
- Atherosclerosis and Cardiovascular Diseases
- Animal Genetics and Reproduction
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- Chemokine receptors and signaling
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Zebrafish Biomedical Research Applications
- Birth, Development, and Health
- Wound Healing and Treatments
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- Immune Response and Inflammation
Polish Academy of Sciences
2007-2022
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2003-2022
Polish Academy of Learning
2022
Institute of Immunology
2017-2020
Hudson Institute
2017
John Wiley & Sons (United States)
2017
University of Wrocław
1994-1999
Mesenchymal stromal/stem cells (MSCs) constitute a promising tool in regenerative medicine and can be isolated from different human tissues. However, their biological properties are still not fully characterized. Whereas MSCs tissue exhibit many common characteristics, activity some markers depend on of origin. Understanding the factors that underlie MSC biology should important points for consideration researchers interested clinical application.To characterize during longterm culture from:...
Abstract Background Mesenchymal stem cells (MSCs) secrete a cocktail of growth factors and cytokines, which could promote tissue regeneration wound healing. Therefore, in clinical practice, post-culture MSC supernatant treatment be more attractive alternative to autologous cell transplantation. In this study, we compared the regenerative properties supernatants harvested from four newly established human adipose mesenchymal lines (HATMSCs) derived chronic patients or healthy donors. Methods...
Cell-based therapies using mesenchymal stem cells (MSCs) are a promising tool in bone tissue engineering. Bone regeneration with MSCs involves series of molecular processes leading to the activation osteoinductive cascade supported by bioactive factors, including fibroblast growth factor-2 (FGF-2) and morphogenetic protein-2 (BMP-2). In this study, we examined biological characteristics osteogenic differentiation potential sheep marrow (BM-MSCs) treated 20 ng/mL FGF-2 100 BMP-2 vitro. The...
Vascular endothelial cells recognize blood-borne circulating and allow them to extravasate in a tissue-specific manner. Because this property determines the selectivity of lymphocyte homing, it is fundamental physiological as well pathological processes (inflammation, autoimmune diseases, metastasis). As tool assess molecular basis endothelium selectivity, microvascular cell lines distinct tissue origin were established. Endothelial cells, isolated from lymphoid tissues (lymph nodes...
Abstract Endothelial progenitor cells (EPCs) modulate postnatal vascularization and contribute to vessel regeneration in adults. Stem were found umbilical cord blood, bone marrow, mobilized peripheral blood cells, from where they isolated cultured. However, the yield of is usually not sufficient for clinical application quality varies. The aim study was immortalization early with high proliferative potential, capable differentiate EPCs and, further, toward endothelial cells. Two cell lines,...
Endothelial progenitor cells (EPCs) and mesenchymal stem/stromal (MSCs) are associated with maintaining tissue homeostasis repair. Both types of contribute to regeneration through the secretion trophic factors (alone or in form microvesicles). This study investigated isolation biological properties microvesicles (MVs) derived from human immortalized MSC line HATMSC1 adipose origin EPC line. The cell a patient venous stasis was established our laboratory using hTERT pSV402 plasmids....
Four human colon adenocarcinoma cell line variants with different metastatic potential were used to examine whether a correlation exists between actin level, state of polymerization and invasiveness tumour cells. Monomeric (G), total (T) filamentous (F) determined in the cytosolic fraction these A statistically significant decrease G level increase (measured by F:G ratio) found cytosol three higher (EB3, 3LNLN, 5W) compared parental (LS180). Our experimental data lead conclusion that there...
Abstract Skin injury leading to chronic wounds is of high interest due the increasing number patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in wound healing processes. For that reason, controlled promotion these processes required. In work, we present portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system gas temperature for biological activities. To make He-DBD-based safe purposes, a multivariate optimization...
Mesenchymal stem cells (MSCs) attract interest in regenerative medicine for their potential application bone regeneration. However, direct transplantation of into damaged tissue is not efficient enough to regenerate large defects. This problem could be solved with a biocompatible scaffold. Consequently, engineering constructs based on biomaterial scaffolds, MSCs, and osteogenic cytokines are promising tools The aim this study was evaluate the effect FGF-2 BMP-2 ovine marrow-derived MSCs...
Active cellular transporters of harmful agents—multidrug resistance (mdr) proteins—are present in tumor, stem and endothelial cells, among others. While mdr proteins are broadly studied tumor their role non-tumor cells the significance action not connected with removal xenobiotics is less extensively documented. Proper assessment expression difficult. Mdr mRNA presence most often evaluated but that does necessarily correlate protein level. The itself difficult to determine; usually...
Transport of bioactive cargo microvesicles (MVs) into target cells can affect their fate and behavior change microenvironment. We assessed the effect MVs derived from human immortalized mesenchymal stem adipose tissue-origin (HATMSC2-MVs) on biological activity ovarian cancer cell lines ES-2 (clear carcinoma) OAW-42 (cystadenocarcinoma). The HATMSC2-MVs were characterized using dynamic light scattering (DLS), transmission electron microscopy, flow cytometry. anti-tumor properties MTT for...
Cells of transplantable MC38 colon carcinoma C57BL/6 mice were adapted to growth in vitro as the MC38/0 cell line. Along establishing process, cells preserved their tumorigenicity. After transduction with a retroviral vector carrying murine interleukin 12 (mIL-12) genes and further selection, stable MC38/IL-12 transductant obtained. These produced IL-12 (approx. 2500 ng/ml/5x10(5) cells/48 h) evaluated optimized bioassay. subcutaneous inoculation into syngeneic mice, IL-12-modified...
Psoriasis is associated with an increased cardiovascular risk. Circulating endothelial progenitor cells (CEPCs) play a significant role in the maintenance of vascular homeostasis.The aim this study was to evaluate number CEPCs patients psoriasis compared controls and assess possible correlations between these plasma levels growth factor (VEGF), soluble receptor-1 (sVEGFR-1) clinical features psoriasis.The CEPCs, identified as CD133+/KDR+ cells, determined flow cytometry peripheral blood...
Mesenchymal stem cells (MSCs) can improve chronic wound healing; however, recent studies suggest that the therapeutic effect of MSCs is mediated mainly through growth factors and cytokines secreted by these cells, referred to as MSC secretome. To overcome difficulties related translation cell therapy into clinical use such efficacy, safety cost, we propose a hydrogel loaded with secretome from recently established human adipose tissue mesenchymal line (HATMSC2) potential treatment for...
Abstract Background Numerous studies have demonstrated increased cardiovascular risk in psoriasis. Circulating endothelial cells (CECs) been proposed as a new marker of dysfunction that plays an important role pathogenesis atherosclerosis. Objective The aim this study was to compare the number CECs psoriatic patients control group and analyze possible correlations between numbers plasma levels classical markers dysfunction, such as: sICAM‐1, sE‐selectin von Willebrand factor (vWF). Methods...
For twenty-five years, attempts have been made to use MSCs in the treatment of various diseases due their regenerative and immunomodulatory properties. However, results are not satisfactory. Assuming that can be replaced some therapies by active factors they produce, immortalized line was established from human adipose tissue (HATMSC1) produce conditioned media test its potential vitro terms possible clinical application. The production biologically primary lower compared HATMSC1 cell...
Abstract Background It is well established that expression of multi‐drug resistance ( MDR ) proteins 1, BCRP , 3, MRP and LRP in leukemic blasts correlates with acute myeloid leukemia AML patients’ clinical response. Assuming stem cells LSC are resistant to chemotherapy responsible for relapse, it might be clinically relevant evaluate the level relate outcome. Methods Bone marrow samples from 26 patients de novo were labeled antibodies distinguish CD 34+ 38− 123+ population against or...
Abstract Rapid endothelialization of cardiovascular stents is critical to prevent major clinical complications such as restenosis. Reconstruction the native endothelium on stent surface can be achieved by capture endothelial progenitor cells (EPCs) or neighboring (ECs) in vivo . In this study, stainless steel were functionalized with recombinant scFv antibody fragments specific for vascular growth factor receptor‐2 (VEGFR2) that expressed EPCs and ECs. Anti‐VEGFR2 scFvs glycosylated form...
Human stem and progenitor cells have recently become objects of intensive studies as an important target for gene therapy regenerative medicine. Retroviral vectors are among the most effective tools genetic modification these cells. However, their transduction efficiency strongly depends on choice ex vivo system. The aim this study was to elaborate a system retroviral vector human CD34 positive isolated from cord blood. pMINV EGFP chosen two erythroblastoid cell lines: KG-1a (CD34 positive)...