A5073179893- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- thermodynamics and calorimetric analyses
- Neonatal Respiratory Health Research
- Inflammatory Bowel Disease
- Advanced Proteomics Techniques and Applications
- Glaucoma and retinal disorders
- Blood properties and coagulation
- Biocrusts and Microbial Ecology
- Force Microscopy Techniques and Applications
- Biotin and Related Studies
- Glycosylation and Glycoproteins Research
- Systemic Lupus Erythematosus Research
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Image Analysis Techniques
- Ubiquitin and proteasome pathways
- Inhalation and Respiratory Drug Delivery
- Microscopic Colitis
- Cardiomyopathy and Myosin Studies
- Erythrocyte Function and Pathophysiology
- Retinal Diseases and Treatments
- Hemoglobin structure and function
- Metabolism, Diabetes, and Cancer
Friedrich-Alexander-Universität Erlangen-Nürnberg
2010-2022
Schiller International University
2013
Fischer (Germany)
2009
Vanderbilt University
2008
The University of Texas Southwestern Medical Center
2008
University of Tübingen
1988-1999
Erasmus University Rotterdam
1992
Max Planck Society
1990
La Roche College
1981-1982
Universidade de Santiago de Compostela
1981
Thymosin beta 4, recently isolated from calf thymus, is present in a number of rat and mouse tissues, including spleen, brain, lung, liver, heart muscle. High concentrations are found peritoneal macrophages, suggesting that its occurrence other tissues may be related to the presence macrophages or macrophage-like cells these tissues. The conclusion "thymosin" 4 does not originate solely thymus gland supported by high athymic (nu/nu) mice.
Prolonged myocardial ischemia results in cardiomyocyte loss despite successful revascularization. We have reported that retrograde application of embryonic endothelial progenitor cells (eEPCs) provides rapid paracrine protection against ischemia-reperfusion injury. Here, we investigated the role thymosin beta4 (Tbeta4) as a mediator eEPC-mediated cardioprotection.In vitro, neonatal rat cardiomyocytes were subjected to hypoxia-reoxygenation absence or presence eEPCs with without Tbeta4 short...
Introduction: Thymosin β4, a low molecular weight, naturally-occurring peptide plays vital role in the repair and regeneration of injured cells tissues. After injury, thymosin is released by platelets, macrophages many other cell types to protect tissues from further damage reduce apoptosis, inflammation microbial growth. β4 binds actin promotes migration, including mobilization, differentiation stem/progenitor cells, which form new blood vessels regenerate tissue. also decreases number...
Eyes with pseudoexfoliation syndrome (PEX) frequently show clinical signs of impairment the blood-aqueous barrier. Herein we give an overview recent studies that analyzed barrier in eyes PEX. Methods: The authors review and summarize including quantification aqueous flare PEX using laser cell meter (LFCM; Kowa FC-1000) comparison normal primary open-angle glaucoma (POAG), without secondary (SOAG), quantitative biochemical determination total protein concentration eyes. In addition,...
Thymosin beta4 is a ubiquitous 43 amino acid, 5 kDa polypeptide that an important mediator of cell proliferation, migration, and differentiation. It the most abundant member beta-thymosin family in mammalian tissue regarded as main G-actin sequestering peptide. angiogenic can promote endothelial migration adhesion, tubule formation, aortic ring sprouting, angiogenesis. also accelerates wound healing reduces inflammation when applied dermal wound-healing assays. Using naturally occurring...
It is shown that AcSDKP a new regulator of the hematopoietic system can he generated from thymosin β 4 by one‐step enzymatic cleavage in vitro and vivo. Tβ were both detected bone marrow cells (BMC'). Incubation [ 3 H]Tβ with either intact or lysed BMC led to formation H]AcSDKP whereas labelled tetrapeptide was not degraded under these conditions. Model degradation carried out bacterial enzymes suggests mammalian endoproteinase Asp‐N might be involved through specific Pro‐ 5 Asp peptide bond T .
Thymosin β4 (Tβ4) was extracted forty years agofrom calf thymus. Since then, it has been identified as a G-actin binding protein involved in blood clotting, tissue regeneration, angiogenesis, and anti-inflammatory processes. Tβ4 also implicated tumor metastasis neurodegeneration. However, the precise roles mechanism(s) of action these processes remain largely unknown, with being insufficient to explain multi-actions. Here we identify for first time important role mechanism ferroptosis, an...
Thymosin β4 is regarded as the main G-actin sequestering peptide in cytoplasm of mammalian cells. It also thought to be involved cellular events like cancerogenesis, apoptosis, angiogenesis, blood coagulation and wound healing. has been previously reported localise intracellularly detected by immunofluorescence. can selectively labelled at two its glutamine-residues with fluorescent Oregon Green cadaverine using transglutaminase; however, this labelling does not interfere interaction...
The beta-thymosins constitute a family of highly conserved and extremely water-soluble 5 kDa polypeptides. Thymosin beta4 is the most abundant member; it expressed in cell types regarded as main intracellular G-actin sequestering peptide. There increasing evidence for extracellular functions thymosin beta4. For example, increases rate attachment spreading endothelial cells on matrix components stimulates migration human umbilical vein cells. Here we show that can be cross-linked to proteins...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolution conformation of thymosin .beta.4: a nuclear magnetic resonance and simulated annealing studyJutta Zarbock, Hartmut Oschkinat, Ewald Hannappel, Hubert Kalbacher, Wolfgang Voelter, Tadeusz A. HolakCite this: Biochemistry 1990, 29, 34, 7814–7821Publication Date (Print):August 28, 1990Publication History Published online1 May 2002Published inissue 28 August 1990https://doi.org/10.1021/bi00486a006RIGHTS & PERMISSIONSArticle...
Objective To study the immediate effects of exogenous surfactant therapy on blood gases, lung volumes, and mechanics in adult rabbits with experimentally induced respiratory distress syndrome. Design Prospective randomized, controlled study. Setting Laboratory animal facility a large university. Subjects Twelve New Zealand white rabbits. Interventions Respiratory failure was by repeated bilateral whole-lung lavage saline (30 mL/kg body weight). After last lavage, animals were randomly...
Two new peptides, designated thymosin beta 8 and 9, respectively, have been isolated their amino acid sequences established. Thymosin 8, from calf thymus fraction 5, has a mass of 4518 daltons contains 39 residues, which 31 are identical to the corresponding residues in 4 same source. The NH2 terminus is acetylalanine, compared with acetylserine 4. fresh-frozen by procedure that minimizes proteolysis, except for presence an additional dipeptide, -Ala-LysOH, at COOH terminus. It 4717 32 its...
Surfactant replacement therapy may be a promising approach for treatment of respiratory failure caused by viral pneumonia. This study in mice demonstrates that during the development lethal influenza A pneumonia, thorax-lung compliance (Ctl/kg) and lung volume at 5 cm H2O PEEP (V5/kg) significantly decrease (28 54%, respectively), whereas water content increases (25%). end stage pneumonia Ctl/kg (31%) V5/kg (21%). Instillation vehicle surfactant control animals does not affect (5% decrease),...
Thymosin beta 4, previously identified as a component of thymosin fraction 5 isolated from calf thymus, is synthesized by peritoneal macrophages derived rats or mice, including cells nu/nu mice. Identification 4 containing [35S]methionine was based on its retention time in reverse-phase HPLC and the recovery radioactivity peptides generated mild acid hydrolysis digestion with trypsin. also rat mouse spleen cells, evidence presented for release these cells.