A5066484497- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Catalysis and Immobilization
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Interaction Studies and Fluorescence Analysis
- RNA modifications and cancer
- Microfluidic and Bio-sensing Technologies
- Polymer Surface Interaction Studies
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Polyamine Metabolism and Applications
- Biochemical and Molecular Research
- Spectroscopy and Quantum Chemical Studies
- Micro and Nano Robotics
- biodegradable polymer synthesis and properties
- Hydrogels: synthesis, properties, applications
- Advancements in Transdermal Drug Delivery
- Protist diversity and phylogeny
- Ocular Surface and Contact Lens
- 3D Printing in Biomedical Research
- Alzheimer's disease research and treatments
- Biopolymer Synthesis and Applications
- Birth, Development, and Health
- Silk-based biomaterials and applications
National Institute of Advanced Industrial Science and Technology
2016-2022
Suita Municipal Hospital
2022
Biomedical Research Institute
2019
University of Tsukuba
2012-2016
Artificial enzyme activators are of great interest for applications in a wide range research fields. Here, we report an hyperactivation system using polyelectrolytes that complementary to charged substrates. The activity α-chymotrypsin (ChT) cationic substrate increased 7-fold at pH 7.0 the presence anionic poly(acrylic acid) (PAAc) and 18-fold poly(allylamine) (PAA). Analysis salt effects, kinetics, dynamic light scattering (DLS), circular dichroism (CD) indicated activation results from...
The development of technology for on/off switching enzyme activity is expected to expand the applications in a wide range research fields. We have previously developed complementary polymer pair system (CPPS) that enables several enzymes be controlled by oppositely charged polymers. However, it failed control large and unstable α-amylase because aggregation complex between anionic cationic poly(allylamine) (PAA) induced irreversible denaturation enzyme. To address this issue, we herein...
Noncovalent binding of polyethylene glycol (PEG) to a protein surface is unique handling technique control function and stability. A diblock copolymer containing PEG polyelectrolyte chains (PEGylated polyelectrolyte) promising candidate for noncovalent attachment because the through multiple electrostatic interactions without denaturation. To obtain deeper understanding protein–polyelectrolyte interaction at molecular level, we investigated manner in which cationic PEGylated binds anionic...
A bifunctional linker molecule containing nitrogen mustard and a cyclic disulfide group has been developed for the covalent immobilization of intact DNA, which allows quantitative analysis epigenomic modification in immobilized DNA using SPR-based immune sensing.
The determination of epigenetic modification, especially that 5-methylcytosine in the CpG sequence mammals, has attracted attention because it should prove valuable a wide range research fields including diagnosis, drug discovery and therapy. In this review, we introduce recent development bisulfite-free DNA methylation analysis, which classify into two categories, namely labelling-based labelling-free assays.
The protein microswimmer, composed of serum albumin, magnetic nanoparticles, and cell-capturing parts, can capture cells, transport them towards target destinations, release at sites in a controllable manner.
Cypridina luciferase is a bioluminescent enzyme that has been used as reporter either in gene expression assays or immunoassays accompanied by an antibody. To develop novel assay for the detection of 5-hydroxymethylcytosine, we first conjugated to antibody against 5-hydroxymethylcytosine. Next, performed modifications guanine bases genome DNA samples with 4-azidophenylglyoxal and biotinylation via azide-Staudinger ligation, which allowed streptavidin capture immobilize under mild conditions....
Since the discovery of active DNA demethylation pathway in mammals, numerous efforts have been made to distinguish epigenetic cytosine variants, including 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). However, rapid discrimination multiple variants remains challenging because conventional assays require time-consuming pretreatments, such as enzymatical digestion chemical conversion. Here we demonstrated high-throughput four by...
Abstract Immobilization of DNA is an important step in relation to DNA‐based biosensors and bioassays with multiple applications. This unit describes synthesis applications novel bifunctional linker molecules containing nitrogen mustard one two types functional groups: cyclic disulfide or biotin. Two ways immobilizing on a surface are described. With the first method, alkylating molecule reacted target form alkylated then immobilized specific surface. second attached surface, through...
Flow imaging microscopy (FIM) is widely used to characterize biopharmaceutical subvisible particles (SVPs). The segmentation threshold, which defines the boundary between particle and background based on pixel intensity, should be properly set for accurate SVP quantification. However, thresholds are often subjectively empirically set, potentially leading variations in measurements across instruments operators. In present study, we developed an objective method optimize FIM threshold using...