A5087264992- Smoking Behavior and Cessation
- Heat shock proteins research
- Carcinogens and Genotoxicity Assessment
- Analytical Chemistry and Chromatography
- Indoor Air Quality and Microbial Exposure
- Potato Plant Research
- Nutrition, Genetics, and Disease
- Toxic Organic Pollutants Impact
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Biochemical effects in animals
- Analytical chemistry methods development
- Cardiac Arrest and Resuscitation
- Autoimmune and Inflammatory Disorders Research
- Acute Myocardial Infarction Research
- Pharmacology and Obesity Treatment
- Pain Mechanisms and Treatments
- Endoplasmic Reticulum Stress and Disease
- Effects and risks of endocrine disrupting chemicals
- Multisensory perception and integration
- Pesticide Residue Analysis and Safety
- Calpain Protease Function and Regulation
- Traumatic Brain Injury Research
- Pharmacogenetics and Drug Metabolism
- Neuroscience and Music Perception
Klinikum rechts der Isar
2024
Technical University of Munich
2024
Virginia Tech
2020-2021
Mechanics' Institute
2020
Austrian Competence Centre of Food Safety
2017-2019
Bemidji State University
2018
University of Kansas
2008-2013
Biocrates Life Sciences (Austria)
2010-2011
Slovak University of Technology in Bratislava
2011
Analytisches Laboratorium
2003-2010
Acrylamide, used in the manufacture of polyacrylamide and grouting agents, is also present diet tobacco smoke. It a neurotoxin probable human carcinogen. Analytical methods were established to determine mercapturic acids acrylamide (N-acetyl-S-(2-carbamoylethyl)-L-cysteine, AAMA) its metabolite glycidamide (N-(R/S)-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-L-cysteine, GAMA) by high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS), as well N-terminal valine adduct...
Increasing the expression of Hsp70 (heat-shock protein 70) can inhibit sensory neuron degeneration after axotomy. Since onset DPN (diabetic peripheral neuropathy) is associated with gradual decline function, we evaluated whether increasing was sufficient to improve several indices neuronal function. Hsp90 master regulator heat-shock response and its inhibition up-regulate Hsp70. KU-32...
Impaired neuronal mitochondrial bioenergetics contributes to the pathophysiologic progression of diabetic peripheral neuropathy (DPN) and may be a focal point for disease management. We have demonstrated that modulating heat shock protein (Hsp) 90 Hsp70 with small-molecule drug KU-32 ameliorates psychosensory, electrophysiologic, morphologic, bioenergetic deficits DPN in animal models type 1 diabetes. The current study used mouse 2 diabetes determine relationship changes sensory neuron onset...
The objective was to evaluate the utility of urinary 1-hydroxypyrene (1-OHP), S-phenylmercapturic acid (S-PMA), trans,trans-muconic (t,t-MA), 3-methyladenine (3-MeAd), 3-ethyladenine (3-EtAd), 8-hydroxy-2′-deoxyguanosine (8-OHdG) and thioethers as biomarkers for assessing exposure in adult smokers who switched from smoking conventional cigarettes candidate potential reduced products (PREP) or stopped smoking. Two electrically heated systems (EHCSS) were used prototype that have significant...
In this study, a two-dimensional liquid chromatography tandem mass spectrometry method was developed and validated for the determination of pesticide residues contaminants in whole wheat grains oats. The samples were extracted with mixture acetonitrile water injected into LC-MS/MS system without any further clean-up or sample preparation. Samples analyzed four different matrix matched calibrations. Matrix effects evaluated by comparing analyte signals respective standard neat solvent...
Cytochrome P450s (P450s) metabolize a large number of diverse substrates with specific regio- and stereospecificity. A compounds, including nicotine, cotinine, aflatoxin B<sub>1</sub>, are metabolites the 94% identical CYP2A13 CYP2A6 enzymes but at different rates. Phenacetin 4-aminobiphenyl were identified as human cytochromes P450 1A2 2A13 not CYP2A6. The purpose this study was to identify active site amino acids that responsible for CYP2A substrate specificity using phenacetin structural...
Crotonaldehyde, an αβ-unsaturated aldehyde, and a potent alkylating agent, is present in many foods beverages, ambient air tobacco smoke. A previous study indicated that two metabolites, 3-hydroxy-1- methylpropylmercapturic acid (HMPMA) 2-carboxy1-1-methylethylmercapturic (CMEMA), were excreted rat urine after subcutaneous injection of crotonaldehyde. Herein, we report the development method based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) deuterated analytes as...
Long-term neuropsychiatric impairments have become a growing concern following blast-related traumatic brain injury (bTBI) in active military personnel and Veterans. Neuropsychiatric such as anxiety depression are common comorbidities that Veterans report months, even years injury. To understand these chronic behavioral outcomes blast injury, there is need to study the link between anxiety, depression, neuropathology. The hippocampus motor cortex (MC) been regions of interest when studying...
Charcoal (CC) filters of cigarettes are known to significantly reduce a series volatile constituents in mainstream smoke, including reactive α,β-unsaturated aldehydes such as acrolein and crotonaldehyde. We performed randomized, crossover, 2-wk brand-switching study with 39 smokers. Twenty the subjects smoked cellulose acetate (CA) filter tipped during wk 1 study; remaining 19 CC 1. In 2, switched corresponding brand other type, similar smoking machine-derived tar nicotine yields. Daily...
Vestibular impairment has become a frequent consequence following blast-related traumatic brain injury (bTBI) in military personnel and Veterans. Behavioral outcomes such as depression, fear anxiety are also common comorbidities of bTBI. To accelerate pre-clinical research therapy developments, there is need to study the link between behavioral patterns neuropathology. The transmission neurosensory information often involves pathway from cerebral cortex thalamus, thalamus serves crucial...
Smoking conventional lit-end cigarettes results in exposure of nonsmokers to potentially harmful cigarette smoke constituents present environmental tobacco (ETS) generated by sidestream emissions and exhaled mainstream smoke. ETS constituent concentrations a newly developed electrically heated smoking system (EHCSS) that produces only no were investigated simulated "office" "hospitality" environments with different levels baseline indoor air quality. the EHCSS (International Organisation for...
The tobacco-specific nitrosamine N′-nitrosonornicotine (NNN) is carcinogenic to humans (IARC Group 1). Assessing the tobacco smoke-related exposure NNN by suitable biomarkers of interest for risk evaluation. Recently, and NNN-N-glucuronide have been quantified in urine smokers. However, it unknown what percentage absorbed dose excreted as total (sum free conjugated NNN) We developed a sensitive method based on liquid chromatography with tandem mass spectrometry deuterium-labeled internal...
This mixed-method study investigates the effects of interactivity in animationsof a molecular-level process and explores perceptions aboutthe animated learning tool used. Treatments were based on principlesof cognitive psychology designed to main treatmentand spatial ability their interaction. Results with students (n=189)showed that science majors scored higher than non-science inretention measures (i.e., structure function) but not transfer.Significant found for treatment function...