A5084591171- Leptospirosis research and findings
- Viral Infections and Vectors
- Syphilis Diagnosis and Treatment
- Reproductive tract infections research
- Veterinary medicine and infectious diseases
- Zoonotic diseases and public health
- Physical Education and Sports Studies
- Yersinia bacterium, plague, ectoparasites research
- Viral gastroenteritis research and epidemiology
- Sex work and related issues
- Business and Management Studies
- Adventure Sports and Sensation Seeking
- RNA and protein synthesis mechanisms
- HIV, TB, and STIs Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Education and Public Policy
- Antimicrobial Peptides and Activities
UConn Health
2020-2025
University of Connecticut
2023-2024
Universidade Federal de Pelotas
2017-2022
The increase in syphilis rates worldwide necessitates development of a vaccine with global efficacy. We aimed to explore Treponema pallidum subspecies (TPA) molecular epidemiology essential for research by analysing clinical data and specimens from early patients using whole-genome sequencing (WGS) publicly available WGS data.
Abstract The development of therapies and vaccines targeting integral membrane proteins has been complicated by their extensive hydrophobic surfaces, which can make production structural characterization difficult. Here we describe a general deep learning-based design approach for solubilizing native while preserving sequence, fold, function using genetically encoded de novo protein WRAPs ( W ater-soluble R Fdiffused A mphipathic P roteins) that surround the lipid-interacting rendering them...
Leptospirosis is a neglected disease of man and animals that affects nearly half million people annually causes considerable economic losses. Current human vaccines are inactivated whole-cell preparations (bacterins) Leptospira spp. provide strong homologous protection yet fail to induce cross-protective immune response. Yearly boosters required, serious side-effects frequently reported so the vaccine licensed for use in humans only handful countries. Novel universal require identification...
Abstract Background The global resurgence of syphilis necessitates vaccine development. Methods We collected ulcer exudates and blood from 17 participants with primary (PS) skin biopsies 51 patients secondary (SS) in Guangzhou, China, for Treponema pallidum subsp (TPA) quantitative polymerase chain reaction, whole genome sequencing (WGS), isolation TPA rabbits. Results DNA was detected 15 3 PS specimens. 50 SS 27 isolated 47 rabbits success rates 71% (12/17) 69% (35/51), respectively,...
Background Venereal syphilis, caused by the spirochete Treponema pallidum subsp. ( TPA ), is surging worldwide, underscoring need for a vaccine with global efficacy. Vaccine development requires an understanding of syphilis epidemiology and clinical presentation as well genomic characterization strains circulating within at-risk populations. The aim this study was to describe clinical, demographic, molecular features early cases in Cali, Colombia. Methods findings We conducted...
Leptospirosis is a zoonosis of worldwide distribution, caused by infection with pathogenic Leptospira species. The vaccines that are currently available bacterins, limited human use, confer short-term, serovar-specific immunity. Lig proteins considered to be the best vaccine candidates date. Here, we aimed construct recombinant chimera (LC) comprised LigAni and LigBrep fragments, evaluate it as subunit or DNA using different administration strategies. Vaccines were formulated 50 µg LC...
Leptospira interrogans, the causative agent of most cases human leptospirosis, must respond to myriad environmental signals during its free-living and pathogenic lifestyles. Previously, we compared L. interrogans cultivated in vitro vivo using a dialysis membrane chamber (DMC) peritoneal implant model. From these studies emerged importance genes encoding Peroxide responsive regulators PerRA PerRB. First described Bacillus subtilis, PerRs are widespread Gram-negative -positive bacteria, where...
The continuing increase in syphilis rates worldwide necessitates development of a vaccine with global efficacy. We conducted multi-center, observational study to explore
As ações municipais no campo do esporte e lazer são multifacetadas. O objetivo desse texto é verificar as de da Prefeitura Municipal Pelotas-RS, na gestão 2009-2012. Recolhemos dados diversas secretarias que alguma forma atuam lazer, bem como Plano Diretor município Pelotas/RS. Entrevistas com envolvidos observação dos espaços públicos práticas esportes também foram realizadas. Concluímos a existência alguns programas não dão conta das necessidades população, precariedade equipamentos praças...
The Omp85 family of outer membrane proteins are ubiquitously distributed among diderm bacteria and play essential roles in (OM) biogenesis. majority orthologs bipartite consist a conserved OM-embedded 16-stranded beta-barrel variable periplasmic functional domains. Here, we demonstrate that Leptospira interrogans encodes four distinct proteins. presumptive leptospiral BamA, LIC11623, contains noncanonical POTRA4 domain is across Leptospiraceae. remaining three proteins, LIC12252, LIC12254...
Abstract Leptospira interrogans , the causative agent of most cases human leptospirosis, must respond to myriad environmental signals during its free-living and pathogenic lifestyles. Previously, we compared L. cultivated in vitro vivo using a dialysis membrane chamber (DMC) peritoneal implant model. From these studies emerged importance genes encoding Peroxide responsive regulators PerRA PerRB. First described Bacillus subtilis PerRs are widespread Gram-negative -positive bacteria, where...
Abstract Leptospirosis is an emerging infectious disease caused by pathogenic Leptospira spp. A universal vaccine against leptospirosis likely to require highly conserved epitopes from leptospires, that are exposed on the bacterial surface, and generate a protective sterilizing immune response. Our group recently identified several genes predicted encode TonB-dependent receptors (TBDR) in interrogans using reverse vaccinology approach. Three leptospiral TBDRs were previously described...