A5080525079- Leptospirosis research and findings
- Veterinary medicine and infectious diseases
- Viral Infections and Vectors
- Tuberculosis Research and Epidemiology
- Immune responses and vaccinations
- Zoonotic diseases and public health
- Mycobacterium research and diagnosis
- Telomeres, Telomerase, and Senescence
- Antimicrobial Peptides and Activities
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Nanoparticles: synthesis and applications
- CRISPR and Genetic Engineering
- Essential Oils and Antimicrobial Activity
- Bacterial biofilms and quorum sensing
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Virology and Viral Diseases
- Antimicrobial Resistance in Staphylococcus
- Anesthesia and Neurotoxicity Research
- Antibiotic Use and Resistance
- Birth, Development, and Health
- Extracellular vesicles in disease
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 detection and testing
Universidade Federal de Pelotas
2016-2025
Universidade Federal de Juiz de Fora
2021-2024
The identification of potential vaccine candidates against leptospirosis remains a challenge. However, one such candidate is OmpL37, potentially surface-exposed antigen that has the highest elastin-binding ability described to date, suggesting it plays an important role in host colonization. In order evaluate OmpL37's induce protective immune response, prime-boost, DNA and subunit strategies were tested hamster model lethal leptospirosis. humoral response was evaluated using indirect ELISA...
The search for environmental preservation and conservation of natural resources gives rise to new concepts viable technical solutions on the path sustainable development. In this context, study’s main objective is analyse influence recycled concrete aggregates (RCAs) development pervious concrete, whose use as a floor covering represents an excellent device mitigate urban soil sealing phenomena. For this, mechanical hydraulic tests were carried out, in addition microstructural analyses...
ABSTRACT Toward developing an effective vaccine capable of conferring heterologous protection, the putative lipoprotein LemA, which presents M3 epitope similar to that Listeria , was evaluated as a candidate in hamster model leptospirosis. LemA is conserved (>70% pairwise identity) among pathogenic Leptospira spp., indicating its potential stimulating cross-protective immune response. Using different vaccination strategies, including prime-boost, DNA vaccine, and subunit preparation,...
Leptospirosis is a zoonosis that responsible for one million human cases per year. Fusing multiple immunogenic antigens represents promising approach to delivering an effective vaccine against leptospirosis. Mycobacterium bovis bacillus Calmette-Guérin (BCG) potential vector due its adjuvant properties and safety. Two chimeric genes based on genic sequences of ligANI, ligBrep, lipL32, lemA, were individually cloned into five BioBrick vectors with different promoters (pAN, Hsp60, 18 kDa,...
Recombinant subunit vaccines have been extensively evaluated as promising alternatives against leptospirosis. Here, we two proteins in formulations containing the adjuvant AddaVax™ vaccine candidates for prevention and control of leptospirosis.Recombinant rErp Y-like rLemA were characterized by ELISA to assess their ability bind extracellular matrix (ECM) components fibrinogen. Groups eight hamsters immunized intramuscularly with or mixed a squalene-based (AddaVax), then efficacy was...
Developing metal-free electrodes for prototypes of bio-based devices is an essential step in producing non-toxic components implantable and wearables. In particular, the advancement self-powered a hot topic several applications due to possibility creating free-battery sensors. this paper, modification bacterial cellulose by progressive incorporation carbon black (a conductive filler) was explored as prototype triboelectric nanogenerators. This process controlled percolation pathways’...
Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the Leptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated N-terminal region leptospiral immunoglobulin-like B protein (LigBrep) as vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, vaccine, and subunit vaccine. Upon challenge virulent strain ofLeptospira interrogans, prime-boost approaches induced significant protection in hamsters,...
Senescent cells have been linked to the pathogenesis of metabolic dysfunction-associated steatotic liver disease (MASLD). However, effectiveness senolytic drugs in reducing damage mice with MASLD is not clear. Additionally, has reported adversely affect male reproductive function. Therefore, this study aimed evaluate protective effect on and fertility MASLD. Three-month-old were fed a standard diet (SD) or choline-deficient western (WD) until 9 months age. At 6 age randomized within dietary...
Abstract Background Leptospirosis, a zoonosis caused by Leptospira spp., is recognized as an emergent infectious disease. Due to the lack of adequate diagnostic tools, vaccines are attractive intervention strategy. Recombinant proteins produced in Escherichia coli have demonstrated promising results, albeit with variable efficacy. Pichia pastoris alternative host several advantages for production recombinant proteins. Results The vaccine candidates LigANI and LipL32 were cloned expressed P....
Immunisation with the C-terminal region of leptospiral immunoglobulin-like A protein (LigANI) has shown promising results against leptospirosis. We evaluated humoral immune response and protection induced by LigANI associated carboxyl multi-walled carbon nanotubes (COOH-MWCNTs), CpG oligodeoxynucleotides (CpG ODNs), or Alhydrogel. Animals immunised ODNs were unable to develop a response, whereas immunisation COOH-MWCNTs produced high level IgG antibodies, similar that Alhydrogel, but it was...
Araçá (Psidium cattleianum Sabine) is a native fruit of South America, with high levels bioactive compounds as well antimicrobial potential. The aim this study was to evaluate the antibacterial activity hydroalcoholic extract araçá (HEA) against Staphylococcus aureus and its mechanism action. HEA showed S. aureus, inhibition halos 17 mm in diameter. minimum inhibitory concentration (MIC) bactericidal (MBC) values were 9 mg mL−1 18 mL−1, respectively. bacterial multiplication inhibited by...
In December 2019, the Chinese Center for Disease Control (CDC of China) reported an outbreak pneumonia in city Wuhan (Hubei province, that haunted world, resulting a global pandemic. This was caused by betacoronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Several these cases have been observed healthcare professionals working hospitals and providing care on pandemic's frontline. present study, nasopharyngeal swab samples workers were used to assess performance...