A5007198423- Leptospirosis research and findings
- Veterinary medicine and infectious diseases
- Antibiotic Resistance in Bacteria
- Viral Infections and Vectors
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Nanoparticles: synthesis and applications
- Essential Oils and Antimicrobial Activity
- Bacterial Infections and Vaccines
- Probiotics and Fermented Foods
- Pneumonia and Respiratory Infections
- Herbal Medicine Research Studies
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Tuberculosis Research and Epidemiology
- Antibiotic Use and Resistance
- Microbial infections and disease research
- Zoonotic diseases and public health
- Bartonella species infections research
- Vibrio bacteria research studies
- Toxin Mechanisms and Immunotoxins
- Animal Virus Infections Studies
Universidade Federal de Pelotas
2016-2025
Universidade Salvador
2011
Leptospirosis is an important neglected infectious disease that occurs in urban environments, as well rural regions worldwide. Rodents, the principal reservoir hosts of pathogenic Leptospira spp., and other infected animals shed bacteria their urine. During occupational or even recreational activities, humans come into direct contact with a contaminated environment, particularly water, are at risk infection. Prevention leptospirosis largely dependent on sanitation measures often difficult to...
The identification of potential vaccine candidates against leptospirosis remains a challenge. However, one such candidate is OmpL37, potentially surface-exposed antigen that has the highest elastin-binding ability described to date, suggesting it plays an important role in host colonization. In order evaluate OmpL37's induce protective immune response, prime-boost, DNA and subunit strategies were tested hamster model lethal leptospirosis. humoral response was evaluated using indirect ELISA...
ABSTRACT Toward developing an effective vaccine capable of conferring heterologous protection, the putative lipoprotein LemA, which presents M3 epitope similar to that Listeria , was evaluated as a candidate in hamster model leptospirosis. LemA is conserved (>70% pairwise identity) among pathogenic Leptospira spp., indicating its potential stimulating cross-protective immune response. Using different vaccination strategies, including prime-boost, DNA vaccine, and subunit preparation,...
ABSTRACT The leptospiral immunoglobulin-like (Lig) proteins LigA and LigB possess domains with 90-amino-acid repeats are adhesion molecules involved in pathogenicity. They conserved pathogenic Leptospira spp. thus of interest for use as serodiagnostic antigens recombinant vaccine formulations. N-terminal amino acid sequences the identical, but C-terminal vary. In this study, we evaluated protective potential five truncated forms from interrogans serovar Canicola DNA vaccines using pTARGET...
Leptospirosis is a zoonosis that responsible for one million human cases per year. Fusing multiple immunogenic antigens represents promising approach to delivering an effective vaccine against leptospirosis. Mycobacterium bovis bacillus Calmette-Guérin (BCG) potential vector due its adjuvant properties and safety. Two chimeric genes based on genic sequences of ligANI, ligBrep, lipL32, lemA, were individually cloned into five BioBrick vectors with different promoters (pAN, Hsp60, 18 kDa,...
Leptospirosis, caused by bacteria of the genus Leptospira , is a direct zoonosis with wide geographical distribution. The implications in terms public health and economical losses leptospirosis justify use vaccine against human or animal populations at risk. In this study, we used external membrane protein LipL32 as model antigen, it highly immunogenic. coding sequence was cloned into several expression vectors: (i) pTarget, to create DNA vaccine; (ii) pUS973, pUS974, pUS977 for BCG (rBCG);...
ABSTRACT Leptospirosis is the most widespread zoonosis in world. Current vaccines are based on whole-cell preparations that cause severe side effects and do not induce satisfactory immunity. In light of leptospiral genome sequences recently made available, several studies aimed at identification protective recombinant immunogens have been performed; however, few such identified. The aim this study was to evaluate 27 antigens determine their potential an immune response against leptospirosis...
Recombinant subunit vaccines have been extensively evaluated as promising alternatives against leptospirosis. Here, we two proteins in formulations containing the adjuvant AddaVax™ vaccine candidates for prevention and control of leptospirosis.Recombinant rErp Y-like rLemA were characterized by ELISA to assess their ability bind extracellular matrix (ECM) components fibrinogen. Groups eight hamsters immunized intramuscularly with or mixed a squalene-based (AddaVax), then efficacy was...
Mycoplasma hyopneumoniae, the etiological agent of swine enzootic pneumonia, is an important pathogen in industry worldwide. Vaccination most cost-effective strategy for controlling and prevention this disease. However, investigations on pathogenicity mechanisms as well current serological detection methods development new recombinant subunit vaccines are hampered by lack known characterized species-specific M. hyopneumoniae antigens. In work, 54 predicted genes encoding proteins with...
Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia and results in significant economic losses swine production worldwide. Vaccination considered to be most cost-effective strategy for control prevention this disease. However, development new recombinant subunit vaccines often hampered by unusual codon usage bacterium. To express M. proteins heterologous systems such as Escherichia coli, TGA codons that encode tryptophan genes need replaced with TGG codon. In study...
Leptospiral immunoglobulin-like (Lig) proteins are of great interest due to their ability act as mediators pathogenesis, serodiagnostic antigens, and immunogens. Purified recombinant LigA protein is the most promising subunit vaccine candidate against leptospirosis reported date, however, purified weak immunogens use a potent adjuvant essential for success vaccine. In present study, we compared xanthan pv. pruni (strain 106), aluminium hydroxide (alhydrogel), CpG ODN adjuvants in...
Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the Leptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated N-terminal region leptospiral immunoglobulin-like B protein (LigBrep) as vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, vaccine, and subunit vaccine. Upon challenge virulent strain ofLeptospira interrogans, prime-boost approaches induced significant protection in hamsters,...