A5058020666- Leptospirosis research and findings
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Veterinary medicine and infectious diseases
- Bacterial biofilms and quorum sensing
- Viral Infections and Vectors
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- Escherichia coli research studies
- Mycotoxins in Agriculture and Food
- Viral gastroenteritis research and epidemiology
- Amphibian and Reptile Biology
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Fish biology, ecology, and behavior
- Aquaculture Nutrition and Growth
- Algal biology and biofuel production
- Antifungal resistance and susceptibility
- Humic Substances and Bio-Organic Studies
- Animal Nutrition and Physiology
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial infections and disease research
Monash University
2011-2016
Australian Regenerative Medicine Institute
2011-2016
Institut Pasteur
2008-2011
Instituto Butantan
2010-2011
Universidade Federal de Pelotas
2003-2010
An understanding of why certain Acinetobacter species are more successful in causing nosocomial infections, transmission and epidemic spread healthcare institutions compared with other is lacking. We used genomic, phenotypic virulence studies to identify differences between species. Fourteen strains representing nine were examined. Genomic analysis six showed that the A. baumannii core genome contains many genes important for diverse metabolism survival host. Most also present one or less...
Acinetobacter baumannii is one of the most notorious hospital-acquired pathogens, and novel treatment strategies are desperately required. Two-component regulatory systems represent potential therapeutic targets as they mediate microorganism adaptation to changing environments, often control virulence, specific bacteria. Here we describe first global virulence regulator in A. baumannii.Using transcriptional profiling functional assays a deletion mutant sensor kinase gene, A1S_0574 (termed...
Significance Acinetobacter baumannii is one of the most significant hospital-acquired bacterial pathogens, able to cause life-threatening infections and develop resistance all currently available antibiotic agents. Here, we established zebrafish as a model study real-time interactions between innate immune cells A. during infection. We identified metabolic pathway that, when inhibited, leads enhanced responses toward bacteria, improving clearance reducing severity disease. The response was...
Leptospira interrogans is the most common cause of leptospirosis in humans and animals. Genetic analysis L. has been severely hindered by a lack tools for genetic manipulation. Recently we developed mariner-based transposon Himar1 to generate first defined mutants interrogans. In this study, total 929 independent were obtained location insertion determined. Of these mutants, 721 located protein coding regions 551 different genes. While sequence sites indicated that transposition occurred an...
Leptospira interrogans is the causative agent of leptospirosis, which an emerging zoonotic disease. Resistance to stress conditions largely uncharacterized for this bacterium. We therefore decided analyze a clpB mutant that we obtained by random transposon mutagenesis. The did not produce any two isoforms ClpB. exhibited growth defects at 30° and 37°C in poor nutrient medium showed increased susceptibility oxidative stress, whereas genetically complemented strain was restored ClpB expression...
The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence lig among serovars from a collection strains clinical isolates. Whilst ligA ligC appeared be present in limited number pathogenic serovars, gene was distributed ubiquitously all strains. None were detected intermediate or saprophytic Leptospira species. It also shown that, similar previously characterized secY gene, short specific fragment could correctly...
Multidrug-resistant (MDR) Acinetobacter baumannii is an opportunistic human pathogen that has become highly problematic in the clinical environment. Novel therapies are desperately required. To assist identifying new therapeutic targets, antagonistic interactions between A. and most common fungal pathogen, Candida albicans, were studied. We have observed C. albicans quorum-sensing molecule, farnesol, cross-kingdom interactions, affecting viability of baumannii. gain understanding its...
Background Leptospirosis is one of the most widespread zoonoses in world and with over 260 pathogenic serovars there an urgent need for a molecular system classification. The development multilocus sequence typing (MLST) schemes Leptospira spp. addressing this issue. aim study was to identify loci potential enhance strain discrimination by sequencing-based methods. Methodology Principal Findings We used bioinformatics evaluate pre-existing increase outbreak strains. Previously deposited data...
The purpose of this study was to perform a 16S sequence-based quality control two Leptospira strain collections. rRNA gene sequencing used verify reference collections provided by the World Health Organization and maintained at laboratory for leptospirosis in Brazil. Among 89 serovars evaluated, four conflicting strains were identified one Although cannot identify beyond species level, it is suitable identification contamination leptospiral This highlights importance availability...
ABSTRACT Acinetobacter baumannii has emerged as a notorious multidrug-resistant pathogen, and development of novel control measures is the utmost importance. Understanding factors that play role in drug resistance may contribute to identification therapeutic targets. Pili are essential for A. adherence biofilm formation on abiotic surfaces well virulence. In present study, we found was significantly induced an imipenem-resistant (Imp r ) strain treated with subinhibitory concentration...
Human and animal leptospirosis caused by Leptospira spp. belonging to serogroup Ballum has increased worldwide in the past decade. We report isolation serologic molecular characterization of four L. borgpetersenii isolates obtained from Mus musculus, preliminary virulence studies. These are useful for diagnosis epidemiologic studies its pathogenic mechanisms.
Background Leptospirosis is a zoonotic infectious disease that affects both humans and animals. The existing genetic tools for Leptospira spp. have improved our understanding of the biology this spirochete as well interaction pathogenic leptospires with mammalian host. However, new are necessary to provide novel useful information field. Methodology Principal Findings A series promoter-probe vectors carrying reporter gene encoding green fluorescent protein (GFP) were constructed use in L....
Our knowledge of the genetics and molecular basis pathogenesis associated with Leptospira, in comparison to those other bacterial species, is very limited. An improved understanding pathogenic mechanisms requires reliable genetic tools for functional analysis. Here, we report expression gfp mRFP1 genes under control constitutive spirochetal promoters both saprophytic Leptospira strains. We were able reliably measure fluorescence by microscopy a fluorometric microplate reader-based assay....
In this study, we observed the presence of antileptospiral agglutinins in freshwater turtles two urban lakes Pelotas, Southern Brazil. Forty animals (29 Trachemys dorbigny and 11 Phrynops hilarii) were captured studied. Attempts to isolate leptospires from blood urine samples unsuccessful. Serum (titer > 100) reactive pathogenic strains animals. These data encourage surveys pet evaluate risk transmission humans.
Mycobacterium bovis BCG has been proposed as an effective live vector for multivalent vaccines.The development of mycobacterial genetic systems to express foreign antigens and the adjuvanticity are basis potential use this attenuated mycobacterium a recombinant vaccine vector.Stable plasmid vectors without antibiotic resistance markers needed heterologous antigen expression in BCG.Our group recently described construction system using auxotrophic complementation selectable marker.In work,...
Rhamdia quelen is an important Neotropical catfish species for fisheries and aquaculture in southern Brazil, where it called Jandia.Like other native Brazilian of economic importance, R. genetics needs more attention animal breeding programs.The growth hormone gene known to be linked a number molecular markers quantitative trait loci.We sequenced the coding region with primer walking technique.As Siluriformes, has four introns five exons, 1465-bp region.The tertiary structure encoded protein...