A5032637148- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- Digital Imaging for Blood Diseases
- Cell Image Analysis Techniques
- Enterobacteriaceae and Cronobacter Research
- Dialysis and Renal Disease Management
- Advanced Chemical Sensor Technologies
- Analytical Chemistry and Sensors
- vaccines and immunoinformatics approaches
- Supramolecular Self-Assembly in Materials
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Integrated Circuits and Semiconductor Failure Analysis
- Cystic Fibrosis Research Advances
- COVID-19 diagnosis using AI
- Identification and Quantification in Food
- Molecular Biology Techniques and Applications
University of California, Los Angeles
2017-2025
California NanoSystems Institute
2019-2023
Samueli Institute
2022
Bioengineering Center
2022
Los Angeles City College
2019
University of California System
2019
Gwangju Institute of Science and Technology
2012-2016
Gwangju University
2014-2015
Government of the Republic of Korea
2015
Korea Research Institute of Bioscience and Biotechnology
2005-2008
We report on chemiluminescence resonance energy transfer (CRET) between graphene nanosheets and chemiluminescent donors. In contrast to fluorescence transfer, CRET occurs via nonradiative dipole-dipole of from a donor suitable acceptor molecule without an external excitation source. designed graphene-based platform for homogeneous immunoassay C-reactive protein (CRP), key marker human inflammation cardiovascular diseases, using luminol/hydrogen peroxide (CL) reaction catalyzed by horseradish...
Paper-based diagnostic devices have many advantages as a one of the multiple test platforms for point-of-care (POC) testing because they simplicity, portability, and cost-effectiveness. However, despite high sensitivity specificity nucleic acid (NAT), development NAT based on paper platform has not progressed much others various specific conditions amplification reactions such pH, buffer components, temperature, inhibitions from technical differences paper-based device. Here, we propose...
One-touch-activated blood multidiagnostic system using a minimally invasive hollow microneedle integrated with paper-based sensor.
Caused by the tick-borne spirochete Borrelia burgdorferi, Lyme disease (LD) is most common vector-borne infectious in North America and Europe. Though timely diagnosis treatment are effective preventing progression, current tests insensitive early stage LD, with a sensitivity of <50%. Additionally, serological testing currently recommended U.S. Center for Disease Control has high costs (>$400/test) extended sample-to-answer timelines (>24 h). To address these challenges, we created...
Abstract We present a deep learning-based framework to design and quantify point-of-care sensors. As use-case, we demonstrated low-cost rapid paper-based vertical flow assay (VFA) for high sensitivity C-Reactive Protein (hsCRP) testing, commonly used assessing risk of cardio-vascular disease (CVD). A machine was developed (1) determine an optimal configuration immunoreaction spots conditions, spatially-multiplexed on sensing membrane, (2) accurately infer target analyte concentration. Using...
A highly rapid, one-step immunoassay of high sensitivity C-reactive protein (hsCRP) using a biosensor with vertical flow (VFA) was developed. The VFA primarily composed sample pad, conjugate FTH film and nitrocellulose (NC) membrane, which were all vertically stacked upon one another. Anti-hsCRP secondary antibodies consecutively immobilized on the NC membrane at position below holes. Gold nanoparticles (AuNPs) conjugated another anti-hsCRP antibody encapsulated in conjugation pad. Various...
We developed a multiplexed point-of-care immunodiagnostic assay for antibody detection in human sera made through the vertical stacking of functional paper layers. In this flow (xVFA), colorimetric signal is generated by gold nanoparticles captured on spatially-multiplexed sensing membrane containing specific antigens. The completed 20 minutes, following which imaged cost-effective mobile-phone reader. images are sent to server, where results rapidly analyzed and relayed back user....
Abstract Multiplexed computational sensing with a point‐of‐care serodiagnosis assay to simultaneously quantify three biomarkers of acute cardiac injury is demonstrated. This sensor includes paper‐based fluorescence vertical flow (fxVFA) processed by low‐cost mobile reader, which quantifies the target through trained neural networks, all within <15 min test time using 50 µL serum sample per patient. fxVFA platform validated human samples biomarkers, i.e., myoglobin, creatine kinase‐MB, and...
We report an interference-free electrochemical lateral-flow immunoassay that enables one-step ultrasensitive detection with serum.
Successful integration of point-of-care testing (POCT) into clinical settings requires improved assay sensitivity and precision to match laboratory standards. Here, we show how innovations in amplified biosensing, imaging, data processing, coupled with deep learning, can help improve POCT. To demonstrate the performance our approach, present a rapid cost-effective paper-based high-sensitivity vertical flow (hs-VFA) for quantitative measurement cardiac troponin I (cTnI), biomarker widely used...
Democratizing biomarker testing at the point-of-care requires innovations that match laboratory-grade sensitivity and precision in an accessible format. Here, high-sensitivity detection of cardiac troponin I (cTnI) is demonstrated through chemiluminescence-based sensing, imaging, deep learning-driven analysis. This chemiluminescence vertical flow assay (CL-VFA) enables rapid, low-cost, precise quantification cTnI, a key protein for assessing heart muscle damage myocardial infarction. The...
We analyzed the aggregation of Alzheimer's β-amyloid (1-42) (Aβ42) peptides from fresh monomers to fully grown fibrils by using in situ surface plasmon resonance (SPR) spectrometry and ex atomic force microscopy (AFM). To immobilize Aβ42 peptide on an SPR chip surface, different carboxy-terminated surfaces were investigated: (1) self-assembled monolayer 11-mercaptoundecanoic acid (2) carboxylated dextran-modified surface. It was found that dextran more appropriate due a much lower degree...
The immunochromatographic (ICA) assay is a highly promising platform for rapid and simple detection of C-reactive protein (CRP) which an indicator the different phases various diseases, as well inflammation infection.However, hook effect in ICA limits quantification CRP levels at high concentrations.Methods: In this study, we developed effect-free (HEF-ICA) to detect over wide concentration range.The results from simultaneous reaction excess target antigens with both immobilized labeled...
Particle agglutination assays are widely adapted immunological tests that based on antigen-antibody interactions. Antibody-coated microscopic particles mixed with a test sample potentially contains the target antigen, as result of which form clusters, size is function antigen concentration and reaction time. Here, we present quantitative particle assay combines mobile lens-free microscopy deep learning for rapidly measuring analyte; its proof-of-concept, demonstrate high-sensitivity...
The rapid spread of SARS-CoV-2 caused the COVID-19 pandemic and accelerated vaccine development to prevent virus control disease. Given sustained high infectivity evolution SARS-CoV-2, there is an ongoing interest in developing serology tests monitor population-level immunity. To address this critical need, we designed a paper-based multiplexed vertical flow assay (xVFA) using five structural proteins detecting IgG IgM antibodies changes immunity levels. Our platform not only tracked...
Point-of-care serological and direct antigen testing offers actionable insights for diagnosing challenging illnesses, empowering distributed health systems. Here, we report a POC-compatible serologic test Lyme disease (LD), leveraging synthetic peptides specific to LD antibodies paper-based platform rapid, cost-effective diagnosis. Antigenic epitopes conserved across Borrelia burgdorferi genospecies, targeted by IgG IgM antibodies, are selected develop multiplexed panel detection of from...