A5109387929- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Advanced Biosensing Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Pharmacological Effects of Natural Compounds
- Analytical Chemistry and Sensors
- Electrowetting and Microfluidic Technologies
- Graphene and Nanomaterials Applications
- Viral Infections and Vectors
- Nanoplatforms for cancer theranostics
- Ginseng Biological Effects and Applications
- Phytochemistry and biological activity of medicinal plants
- Mass Spectrometry Techniques and Applications
- Temporomandibular Joint Disorders
- Molecular Junctions and Nanostructures
- Nanopore and Nanochannel Transport Studies
- Craniofacial Disorders and Treatments
- Orthodontics and Dentofacial Orthopedics
- SARS-CoV-2 detection and testing
- Inflammatory Bowel Disease
State Key Laboratory of Applied Organic Chemistry
2011-2024
Lanzhou University
2011-2024
Capital Medical University
1996-2024
Southeast University
2015-2024
Beijing Friendship Hospital
2017-2024
National Clinical Research Center for Digestive Diseases
2017-2024
Tianjin Normal University
2023
Hubei University
2020-2022
Beijing Children’s Hospital
2022
Harbin University of Commerce
2017-2021
Immunoassays whose readouts rely on chemiluminescence are increasingly useful for a broad range of analytical applications, but they rarely made into point-of-care (POC) format because the complex reagents required (some have to be stored in low temperatures, and some freshly right before assay). This study reports self-contained chemiluminescent lateral flow assay (CLFA), which prestores all necessary reagents. CLFA contains three parts: normal (LFA) strip, substrate pad, polycarbonate (PC)...
Caused by the tick-borne spirochete Borrelia burgdorferi, Lyme disease (LD) is most common vector-borne infectious in North America and Europe. Though timely diagnosis treatment are effective preventing progression, current tests insensitive early stage LD, with a sensitivity of <50%. Additionally, serological testing currently recommended U.S. Center for Disease Control has high costs (>$400/test) extended sample-to-answer timelines (>24 h). To address these challenges, we created...
Abstract Multiplexed analysis of molecules with different concentrations requires assays a tunable detection range. A strategy is outlined that uses click chemistry to assemble horseradish peroxidase in controlled fashion generate enzyme assemblies as probes for multiplexed bioassays. This controllable assembly enzymes on antibodies allows lab‐on‐a‐chip immunoassays range from pg mL −1 μg . Simultaneous, bioassays clinically relevant inflammatory biomarkers serum are demonstrated one format,...
We report an ultrasensitive, quantitative, and rapid bioluminescent immunosensor (ABS) for point-of-care testing (POCT) of the disease biomarker in clinical samples using double enzymes including alkaline phosphatase (ALP) luciferase. In presence biomarker, ALP attached on surface immuno-nanocomplex dephosphorylates adenine triphosphate (ATP), subsequently inhibiting ATP-luciferin-luciferase reaction. The highly sensitive response ATP (picomolar level) allows ultrasensitive detection via...
The purpose of the present study is to evaluate prevalence temporomandibular disorders (TMD) and their associated biological psychological factors in Chinese university students.A total 754 students were included from Zunyi Medical University, each participant completed questionnaires clinical examinations according Diagnostic Criteria for Temporomandibular Disorders.The overall TMD was 31.7% among medical students. Subjects with had a high bruxism, empty chewing, unilateral chewing gum,...
A new, rapid, and sensitive method was proposed for the determination of sulfonamide residues in milk chicken muscle samples by microchip electrophoresis with laser-induced fluorescence detection. Separation fluorescamine-labeled sulfonamides accomplished using a buffer containing 5 mmol/L boric acid 1% (w/v) polyvinyl alcohol (PVA). The pH, amount PVA, concentration running were found to have great influence on separation. By optimizing these conditions, separation four sulfonamides,...
A novel electrochemical homogeneous immunosensor strategy was developed based on proximity hybridization that regulated the RCA.
The colorimetric immunoassay based on gold nanoparticles (AuNPs) can hardly enable simultaneous detection of multiple biomarkers in vastly different concentrations (e.g., pg/mL−μg/mL) because its narrow dynamic range. In this work, we demonstrate an with tunable range by using peptide-mediated controlled aggregation surface modification-free AuNPs. Alkaline phosphatase (ALP) removes the phosphate group peptide to yield a positively charged product, which triggers negatively AuNPs and color...
Magnetic lateral flow strip (MLFS) based on magnetic bead (MB) and smart phone camera has been developed for quantitative detection of cocaine (CC) in urine samples. CC CC-bovine serum albumin (CC-BSA) could competitively react with MB-antibody (MB-Ab) the surface test line MLFS. The color MB-Ab conjugate relates to concentration target competition immunoassay format, which can be used as a visual signal. Furthermore, density transferred into digital signal (gray value) by phone, linear...
A highly sensitive aptasensor for aflatoxin M1 (AFM1) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method exhibited broad strong absorption in the whole ultraviolet-visible (UV-Vis) range. The coordination interaction nitrogen functional groups of AFM1 aptamer brought FAM close proximity, which resulted quenching to maximum...
Bioinspired nanochannels that manipulate ion transport have shown great potential for understanding complex physiological processes. Herein, inspired by the gating function of biological channels, we designed and constructed artificial hydrogen peroxide (H2O2)-activated decorating inner pore surface with 4-(phenoxymethyl) benzeneboronic acid pinacol ester (PBAE). Benefiting from specific hydrolysis reaction between H2O2 PBAE in confined nanochannels, functionalized exhibited a highly...
Current magnetic relaxation switching (MRS) sensors for detection of trace targets in complex samples still suffer from limitations terms relatively low sensitivity and poor stability. To meet this challenge, we develop a longitudinal time (T1)-based nanosensor by using Mn2+ released the reduction MnO2 nanoassembly that can induce change T1, thus greatly improve overcome "hook effect" conventional MRS. Through specific interaction between antigen antibody-functionalized nanoassembly, T1...