A5014210574- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Glioma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cell Image Analysis Techniques
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Single-cell and spatial transcriptomics
- Spinal Cord Injury Research
- Nerve injury and regeneration
- Cancer, Hypoxia, and Metabolism
- COVID-19 diagnosis using AI
- Industrial Automation and Control Systems
- PARP inhibition in cancer therapy
- Pluripotent Stem Cells Research
- Hydrogels: synthesis, properties, applications
- Immune Cell Function and Interaction
- Silk-based biomaterials and applications
San Diego Supercomputer Center
2025
University of California, San Diego
2011-2025
Fluxion Biosciences (United States)
2023-2024
University of California, Los Angeles
2016-2022
UCLA Health
2018-2020
California NanoSystems Institute
2019-2020
La Jolla Bioengineering Institute
2019
Moores Cancer Center
2011
Abstract We present a deep learning-based framework to design and quantify point-of-care sensors. As use-case, we demonstrated low-cost rapid paper-based vertical flow assay (VFA) for high sensitivity C-Reactive Protein (hsCRP) testing, commonly used assessing risk of cardio-vascular disease (CVD). A machine was developed (1) determine an optimal configuration immunoreaction spots conditions, spatially-multiplexed on sensing membrane, (2) accurately infer target analyte concentration. Using...
Abstract Glioblastoma (GBM) tumors exhibit potentially actionable genetic alterations against which targeted therapies have been effective in treatment of other cancers. However, these largely failed GBM patients. A notable example is kinase inhibitors EGFR, display poor clinical efficacy despite overexpression and/or mutation EGFR >50% GBM. In addressing this issue, preclinical models may be limited by the inability to accurately replicate pathophysiologic interactions cells with...
Abstract Neural stem/progenitor cell (NS/PC)‐based therapies have shown exciting potential for regeneration of the central nervous system (CNS) and NS/PC cultures represent an important resource disease modeling drug screening. However, significant challenges limiting clinical translation remain, such as generating large numbers cells required model or transplantation, maintaining physiologically representative phenotypes ex vivo directing differentiation into specific fates. Here, we report...
Abstract The secreted products of cells drive many functions in vivo; however, methods to link this functional information surface markers and transcriptomes have been lacking. By accumulating secretions close secreting held within cavity-containing hydrogel nanovials, we demonstrate workflows analyze the amount IgG from single human B same cells. Measurements using flow cytometry imaging corroborate association between secretion CD38/CD138. oligonucleotide-labeled antibodies find that...
Abstract The easiest and often most useful way to work with experimentally determined or computationally predicted structures of biomolecules is by viewing their three‐dimensional (3D) shapes using a molecular visualization tool. Mol* was collaboratively developed RCSB Protein Data Bank (RCSB PDB, RCSB.org ) in Europe (PDBe, PDBe.org as an open‐source, web‐based, 3D software suite for examination analyses biostructures. It capable displaying atomic coordinates related experimental data...
The spinal cord is unable to regenerate after injury largely due growth-inhibition by an inflammatory response the that fails resolve, resulting in secondary damage and cell death. An approach prevents inhibition attenuating promoting its resolution through transition of macrophages anti-inflammatory phenotypes essential for creation a growth permissive microenvironment. Viral gene delivery induce expression factors provides potential provide localized alter host response. Initially, we...
Chemotherapy resistance to glioblastoma (GBM) remains an obstacle that is difficult overcome, leading poor prognosis of GBM patients. Many previous studies have focused on mechanisms intrinsic cancer cells; the microenvironment surrounding tumor cells has been found more recently significant impacts response chemotherapeutic agents. Extracellular matrix (ECM) proteins may confer cell adhesion-mediated drug (CAMDR). Here, expression ECM laminin, vitronectin, and fibronectin was assessed in...
Biomaterials are being developed as therapeutics for spinal cord injury (SCI) that can stabilize and bridge acute lesions mediate the delivery of transgenes, providing a localized sustained reservoir regenerative factors. For clinical use, direct injection biomaterial scaffolds is preferred to enable conformation unique minimize tissue damage. While an interconnected network cell-sized macropores necessary rapid host cell infiltration into—and thus integration with—implanted scaffolds,...
Antibody discovery technologies, essential for research and therapeutic applications, have evolved significantly since the development of hybridoma technology. Various in vitro (display) vivo (animal immunization B cell-sequencing) workflows led to antibodies against diverse antigens. Despite this success, standard display B-cell sequencing-based technologies are limited targets that can be produced a soluble form. This limitation inhibits screening function-inducing antibodies, which...
Cell-matrix interactions mediate complex physiological processes through biochemical, mechanical, and geometrical cues, influencing pathological changes therapeutic responses. Accounting for matrix effects earlier in the drug development pipeline is expected to increase likelihood of clinical success novel therapeutics. Biomaterial-based strategies recapitulating specific tissue microenvironments 3D cell culture exist but integrating these with 2D methods primarily used screening has been...
ABSTRACT Protein secretion drives many functions in vivo ; however, methods to link secretions with surface markers and transcriptomes have been lacking. By accumulating close secreting cells held within cavity-containing hydrogel nanovials, we demonstrate workflows analyze the amount of IgG secreted from single human antibody-secreting this information marker expression transcriptional profiles same cells. Measurements using flow cytometry imaging corroborated an association between levels...
ABSTRACT We present a deep learning-based framework to design and quantify point-of-care sensors. As its proof-of-concept use-case, we demonstrated low-cost rapid paper-based vertical flow assay (VFA) for high sensitivity C-Reactive Protein (hsCRP) testing, common medical test used quantifying the degree of inflammation in patients at risk cardio-vascular disease (CVD). A machine sensor was developed two key tasks: (1) determine an optimal configuration immunoreaction spots conditions,...
Abstract The therapeutic application of monoclonal antibodies has been invaluable in improving the quality life for countless patients across numerous indications. Despite advances streamlining process, enhanced screening methods still necessitate substantial investments equipment, reagents, and training. To address this gap, we have developed a workflow utilizing Nanovials, hydrogel nanoliter containers, to enable discovery functional from single cells with simple without any new equipment...
Abstract CAR-T therapies, while potent against some cancers, face challenges including toxicities and tumor relapse, particularly in solid tumors with hostile TMEs sub-optimal CAR designs. A major obstacle is the lack of efficient functional screening methods for cells, crucial T-cell-based immunotherapy progress. Our study introduces a novel approach using hydrogel microparticles (Nanovials) to analyze millions individual cells based on antigen specificity cytokine secretion. We modified...
Cell-matrix interactions mediate complex physiological processes through biochemical, mechanical, and geometrical cues, influencing pathological changes therapeutic responses. Accounting for matrix effects earlier in the drug development pipeline is expected to increase likelihood of clinical success novel therapeutics. Biomaterial-based strategies recapitulating specific tissue microenvironments 3D cell culture exist but integrating these with 2D methods primarily used screening has been...
Event Abstract Back to Hyaluronic acid hydrogels for spinal cord regeneration Christopher Walthers1, Jesse Liang1, Joshua Karam1, 2, Arshia Ehsanipour1 and Stepanie K. Seidlits1 1 University of California, Los Angeles, Bioengineering, United States 2 Riverside, Introduction: Many disorders the central nervous system (CNS) cause extensive death highly specialized oligodendrocytes. While endogenous neural stem/progenitor cells (NS/PCs) are recruited diseased areas in adult CNS, their...
<div>Abstract<p>Glioblastoma (GBM) tumors exhibit potentially actionable genetic alterations against which targeted therapies have been effective in treatment of other cancers. However, these largely failed GBM patients. A notable example is kinase inhibitors EGFR, display poor clinical efficacy despite overexpression and/or mutation EGFR >50% GBM. In addressing this issue, preclinical models may be limited by the inability to accurately replicate pathophysiologic interactions...
<p>Supplementary figures S1-S12 providing necessary data on additional animal xenograft experiments, essential information characterizing cell lines used in manuscript, and controls for immunostaining western blots. This supplementary file also provides experiment lines.</p>
<p>Supplementary Methods describing additional information for procedures in manuscript, including hydrogel fabrication, sample cryopreservation, lentivirus preparation, mechanical characterization, diffusion, western blotting, and statistics.</p>
<div>Abstract<p>Glioblastoma (GBM) tumors exhibit potentially actionable genetic alterations against which targeted therapies have been effective in treatment of other cancers. However, these largely failed GBM patients. A notable example is kinase inhibitors EGFR, display poor clinical efficacy despite overexpression and/or mutation EGFR >50% GBM. In addressing this issue, preclinical models may be limited by the inability to accurately replicate pathophysiologic interactions...
<p>Supplementary Methods describing additional information for procedures in manuscript, including hydrogel fabrication, sample cryopreservation, lentivirus preparation, mechanical characterization, diffusion, western blotting, and statistics.</p>