A5066784367- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- RNA regulation and disease
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- interferon and immune responses
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Immune Response and Inflammation
- Renal Transplantation Outcomes and Treatments
- Toxin Mechanisms and Immunotoxins
- Galectins and Cancer Biology
- Musicology and Musical Analysis
- Music History and Culture
- Mosquito-borne diseases and control
- Diabetes and associated disorders
- Protein Tyrosine Phosphatases
- Immunodeficiency and Autoimmune Disorders
- Ocular Diseases and Behçet’s Syndrome
- Medical History and Innovations
- Atherosclerosis and Cardiovascular Diseases
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- RNA modifications and cancer
University of Kansas
2023-2024
University of Michigan–Ann Arbor
2020-2023
Albert Einstein College of Medicine
2019-2022
Mayo Clinic in Arizona
2020
University of Iowa
2015
Retinitis pigmentosa (RP) is a highly heterogeneous group of disorders characterized by degeneration the retinal photoreceptor cells and progressive loss vision. While hundreds mutations in more than 100 genes have been reported to cause RP, discovering causative many patients remains significant challenge. Exome sequencing an individual affected with non-syndromic RP revealed two plausibly disease-causing variants TRNT1, gene encoding nucleotidyltransferase critical for tRNA processing. A...
The immune regulatory gene PTPN22 is expressed in all cells and encodes Lyp humans the ortholog PEP mice. alternative allele, 1858C>T, 5-15% of North American population strongly associated with development autoimmune disease while simultaneously capable providing protection during virus infection cancer. In murine models, significant progress has been made elucidating molecular mechanisms that its pro-autoimmune variant (PEP-R619W) modulate T cell function, yet their influence on non-T...
During infection of neurons by alphaherpesviruses including Pseudorabies virus (PRV) and Herpes simplex type 1 (HSV-1) viral nucleocapsids assemble in the cell nucleus, become enveloped body then traffic into down axons to nerve termini for spread adjacent epithelia. The membrane protein US9p glycoprotein heterodimer gE/gI play critical roles anterograde both HSV-1 PRV, several models exist explain their function. Biochemical studies suggest that PRV associates with kinesin-3 motor KIF1A a...
Capsid envelopment during assembly of the neurotropic herpesviruses herpes simplex virus 1 (HSV-1) and pseudorabies (PRV) in infected cell cytoplasm is thought to involve late-acting cellular ESCRT (endosomal sorting complex required for transport) components ESCRT-III VPS4 (vacuolar protein 4). However, HSV-1, unlike members many other families enveloped viruses, does not appear require ESCRT-I subunit TSG101 or Bro1 domain-containing ALIX (Alg-2-interacting X) recruit activate ESCRT-III....
Host genes define the severity of inflammation and immunity but specific loci doing so are unknown. Here we show that TNF receptor superfamily member 13B (TNFRSF13B) variants, which enhance defense against certain pathogens, also control immune-mediated injury transplants, by regulating innate B cells' functions. Analysis TNFRSF13B in human kidney transplant recipients revealed 33% those with antibody-mediated rejection (AMR) fewer than 6% stable graft function had missense mutations. To...
Transcript analyses highlight an important contribution of natural killer (NK) cells to microvascular inflammation (MVI) in antibody-mediated rejection (ABMR), but only few immunohistologic studies have quantified their spatial distribution within graft tissue. This study included 86 kidney transplant recipients who underwent allograft biopsies for a positive donor-specific antibody (DSA) result. NK were visualized and glomeruli peritubular capillaries (PTC), using immunohistochemistry CD34...
Transplantation of allogeneic donor ovarian tissue holds great potential for female cancer survivors who often experience premature insufficiency. To avoid complications associated with immune suppression and to protect transplanted allografts from immune-mediated injury, we have developed an immunoisolating hydrogel-based capsule that supports the function without triggering response. Encapsulated implanted in naïve ovariectomized BALB/c mice responded circulating gonadotropins maintained 4...
Alphaherpesviruses, including herpes simplex virus 1 (HSV-1) and pseudorabies (PRV), are pathogens of the nervous system. They replicate in nerve cell body then travel great distances along axons to reach termini spread adjacent epithelial cells; however, key aspects how these viruses remain controversial.
Abstract The deadly consequences of viral infection were clearly demonstrated by the COVID-19 pandemic. Therefore, identifying new therapeutic targets to enhance our antiviral immunity and combat virus is essential, such as immune-regulatory gene PTPN22. 5-15% North Americans express a PTPN22 alternative allele which linked with multiple autoimmune diseases. However, there significant research gap regarding role its common during infection. To address this, we used CRISPR/Cas9 generated...
Abstract Dysfunctional dendritic cells (DC) lead to inadequate T cell activation causing chronic virus infection. Enhancing both DC and function can prevent An approach enhance immune is harness the immune-activating phenotype driven by autoimmunity-associated allele of PTPN22, which expressed in all cells. We previously found that mice expressing Ptpn22 (Ptpn22-R619W) successfully clear Lymphocytic choriomeningitis clone 13 (LCMV-cl13) unlike wildtype (WT) mice. This correlates with a more...
Abstract The tyrosine phosphatase PTPN22 encodes the enzyme Lyp (PEP in mice) all immune cells. 5–10% of North American population expresses autoimmunity risk allele 1858C>T (rs2476601) causing amino acid substitution R620W (R619W mice). This mutation alters lymphocyte activation, toll-like receptor signaling, and cytokine production. Ptpn22 knockout (PEP-null) alternative allele-expressing (PEP-R619W) mice clear persistent virus LCMV-cl13, have enhanced anti-viral T cell function, a...
Abstract Transplantation of allogeneic donor ovarian tissue holds great potential for female cancer survivors who often experience premature insufficiency. To avoid complications associated with immune suppression and to protect transplanted allografts from immune-mediated injury, we have developed an immuno-isolating hydrogel-based capsule that supports the function without triggering response. Encapsulated implanted in naïve ovariectomized BALB/c mice responded circulating gonadotropins...