A5066635236- Leprosy Research and Treatment
- Erythrocyte Function and Pathophysiology
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- Autophagy in Disease and Therapy
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Ovarian function and disorders
- Monoclonal and Polyclonal Antibodies Research
- Transplantation: Methods and Outcomes
- T-cell and B-cell Immunology
- Calcium signaling and nucleotide metabolism
- COVID-19 Clinical Research Studies
- Escherichia coli research studies
- Psoriasis: Treatment and Pathogenesis
- Blood disorders and treatments
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Ocular Diseases and Behçet’s Syndrome
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- CAR-T cell therapy research
- Organ and Tissue Transplantation Research
- Sphingolipid Metabolism and Signaling
University of Michigan–Ann Arbor
2018-2024
Michigan United
2021
Fundação Oswaldo Cruz
2014-2021
Central Leprosy Teaching & Research Institute
2017
Faculdades Oswaldo Cruz
2017
Instituto Nacional do Câncer
1992
Leprosy is a chronic infectious disease that may present different clinical forms according to the immune response of host. Levels IFN-γ are significantly raised in paucibacillary tuberculoid (T-lep) when compared with multibacillary lepromatous (L-lep) patients. primes macrophages for inflammatory activation and induces autophagy antimicrobial mechanism. The involvement against Mycobacterium leprae remains unexplored. Here, we demonstrated by autophagic assays LC3-positive autophagosomes...
Abstract The COVID‐19 pandemic continues to be a severe threat human health, especially due current and emerging SARS‐CoV‐2 variants with potential escape humoral immunity developed after vaccination or infection. development of broadly neutralizing antibodies that engage evolutionarily conserved epitopes on coronavirus spike proteins represents promising strategy improve therapy prophylaxis against thereof. Herein, facile multivalent engineering approach is employed achieve large...
ABSTRACT Intracellular Mycobacterium leprae infection modifies host macrophage programming, creating a protective niche for bacterial survival. The milieu regulating cellular apoptosis in the tissue plays an important role defining susceptible and/or resistant phenotypes. A higher density of apoptotic cells has been demonstrated paucibacillary leprosy lesions than multibacillary ones. However, effect cell removal on M. -stimulated yet to be fully elucidated. In this study, we investigated...
The pathways that trigger exacerbated immune reactions in leprosy could be determined by genetic variations. Here, a prospective approach, both and non-genetic variables influencing the amount of time before development reactional episodes were studied using Kaplan-Meier survival curves, effect was estimated Cox proportional-hazards regression model. In sample including 447 patients, we confirmed gender (male), high bacillary clinical forms are risk factors for reactions. From 15 single...
Erythema Nodosum Leprosum (ENL) is an immune reaction in leprosy that aggravates the patient´s clinical condition. ENL presents systemic symptoms of acute infectious syndrome with high leukocytosis and intense malaise clinically similar to sepsis. The treatment patients requires immunosuppression thus needs be early efficient prevent both disabilities permanent nerve damage. Some experience multiple episodes prolonged use immunosuppressive drugs may lead serious adverse effects. Thalidomide...
Abstract Premature ovarian insufficiency (POI) is a significant complication of cytotoxic treatments due to extreme sensitivity chemotherapy and radiation. POI particularly devastating for young girls reaching puberty, because it irreversibly affects their physical cognitive development. Changes occurring during puberty determine height, bone health, insulin responsiveness, lipid metabolism, cardiovascular health cognition. The only available treatment hormone replacement therapy (HRT),...
Abstract Monoclonal antibodies that target SARS-CoV-2 with high affinity are valuable for a wide range of biomedical applications involving novel coronavirus disease (COVID-19) diagnosis, treatment, and prophylactic intervention. Strategies the rapid reliable isolation these antibodies, especially potent neutralizing critical toward improved COVID-19 response informed future to emergent infectious diseases. In this study, single B cell screening was used interrogate antibody repertoires...
Leprosy reactions are responsible for incapacities in leprosy and represent the major cause of permanent neuropathy. The identification biomarkers able to identify patients more prone develop reaction could contribute adequate clinical management prevention incapacities. Reversal may occur unstable borderline also lepromatous patients. To biomarker signature profiles related with reversal onset, multibacillary were recruited classified accordingly occurrence or not during after...
Abs that neutralize SARS-CoV-2 are thought to provide the most immediate and effective treatment for those severely afflicted by this virus. Because coronavirus potentially diversifies mutation, broadly neutralizing especially sought. Here, we report a possibly novel approach rapid generation of potent human anti-SARS-CoV-2 Abs. We isolated spike protein-specific memory B cells panning from blood convalescent subjects after infection with sequenced expressed Ig genes individual as mAbs. All...
Host genes define the severity of inflammation and immunity but specific loci doing so are unknown. Here we show that TNF receptor superfamily member 13B (TNFRSF13B) variants, which enhance defense against certain pathogens, also control immune-mediated injury transplants, by regulating innate B cells' functions. Analysis TNFRSF13B in human kidney transplant recipients revealed 33% those with antibody-mediated rejection (AMR) fewer than 6% stable graft function had missense mutations. To...
Premature loss of ovarian function (POI) is associated with numerous negative side effects, including vasomotor symptoms, sleep and mood disturbances, disrupted urinary function, increased risks for osteoporosis heart disease. Hormone replacement therapy (HRT), the standard care POI, delivers only a subset hormones fails to mimic monthly cyclicity daily pulsatility characteristic healthy tissue in reproductive-aged individuals whose contains thousands follicles. Ovarian allografts have...
Immune-surveillance depends in part on the recognition of peptide variants by T cell antigen receptors. Given that both normal B cells and malignant accumulate mutations we chose a murine model multiple myeloma to test conditions induce cell-mediated immunity targeting plasma (PC) clones but sparing PCs. Revealing novel function for intracellular C3d, discovered C3d engaged responses against bone marrow mice had developed spontaneously. Our results show internalized augments immune...
Transplantation of allogeneic donor ovarian tissue holds great potential for female cancer survivors who often experience premature insufficiency. To avoid complications associated with immune suppression and to protect transplanted allografts from immune-mediated injury, we have developed an immunoisolating hydrogel-based capsule that supports the function without triggering response. Encapsulated implanted in naïve ovariectomized BALB/c mice responded circulating gonadotropins maintained 4...
Leprosy reactional episodes are acute inflammatory events that may occur during the clinical course of disease. Type 1 reaction (T1R) is associated with an increase in neural damage, and understanding molecular pathways related to T1R onset pivotal for development strategies effectively control reaction. Interferon-gamma (IFN-γ) a key cytokine also autophagy induction. Here, we evaluated modulation pathway Mycobacterium leprae-stimulated cells presence or absence IFN-γ. We observed IFN-γ...
The treatment of multibacillary cases leprosy with multidrug therapy (MDT) comprises 12 doses a combination rifampicin, dapsone and clofazimine. Previous studies have described the immunological phenotypic pattern in skin lesions patients. Here, we evaluated effect MDT on cell phenotype Mycobacterium leprae -specific immune response. An analysis demonstrated significant decrease MRS1 (SR-A), CXCL10 (IP-10) IFNG (IFN-γ) gene protein expression after release. Patients were randomized according...
Immune surveillance depends in part on the recognition of peptide variants by T cell antigen receptors. Given that both normal B cells and malignant accumulate mutations we chose a murine model multiple myeloma to test conditions induce cell-mediated immunity targeting plasma (PC) clones but sparing PCs. Revealing previously unknown function for intracellular C3d, found C3d engaged responses against PC bone marrow mice had developed spontaneously. Our results show internalized augments...
Abstract A 35‐year‐old Brazilian woman (gravida 4, para 2) was delivered of a severely anaemic child whose cord red blood cells had strongly positive direct antiglobulin test and who required two exchange transfusions within 24 h birth. Because the emergency situation lack local immunohaematology reference laboratory, phenotype mother specificity relevant antibody could not be determined. Hence, compatible immediately available infant to given repeated with incompatible group 0 Rh‐negative...