A5047305679- Leprosy Research and Treatment
- Mycobacterium research and diagnosis
- Autophagy in Disease and Therapy
- Erythrocyte Function and Pathophysiology
- T-cell and B-cell Immunology
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Psoriasis: Treatment and Pathogenesis
- Calcium signaling and nucleotide metabolism
- Ocular Diseases and Behçet’s Syndrome
- Systemic Lupus Erythematosus Research
- Biomarkers in Disease Mechanisms
- Extracellular vesicles in disease
- Antimicrobial Peptides and Activities
- Long-Term Effects of COVID-19
- Whipple's Disease and Interleukins
- SARS-CoV-2 and COVID-19 Research
- Ocular Surface and Contact Lens
- Acne and Rosacea Treatments and Effects
- Tuberculosis Research and Epidemiology
- Advanced biosensing and bioanalysis techniques
- Sphingolipid Metabolism and Signaling
- Atherosclerosis and Cardiovascular Diseases
University of California, Los Angeles
2019-2023
Fundação Oswaldo Cruz
2017-2021
University of Southern California
2020
Indiana University School of Medicine
2020
Central Leprosy Teaching & Research Institute
2017
Faculdades Oswaldo Cruz
2017
High-throughput single-cell RNA-sequencing (scRNA-seq) methodologies enable characterization of complex biological samples by increasing the number cells that can be profiled contemporaneously. Nevertheless, these approaches recover less information per cell than low-throughput strategies. To accurately report expression key phenotypic features cells, scRNA-seq platforms are needed both high fidelity and throughput. address this need, we created Seq-Well S3 ("Second-Strand Synthesis"), a...
Acne affects 1 in 10 people globally, often resulting disfigurement. The disease involves excess production of lipids, particularly squalene, increased growth Cutibacterium acnes, and a host inflammatory response with foamy macrophages. By combining single-cell spatial RNA sequencing as well ultrahigh-resolution Seq-Scope analyses early acne lesions on back skin, we identified TREM2 macrophages expressing lipid metabolism proinflammatory gene programs proximity to hair follicle epithelium...
Leprosy is a chronic infectious disease that may present different clinical forms according to the immune response of host. Levels IFN-γ are significantly raised in paucibacillary tuberculoid (T-lep) when compared with multibacillary lepromatous (L-lep) patients. primes macrophages for inflammatory activation and induces autophagy antimicrobial mechanism. The involvement against Mycobacterium leprae remains unexplored. Here, we demonstrated by autophagic assays LC3-positive autophagosomes...
SUMMARY The development of high-throughput single-cell RNA-sequencing (scRNA-Seq) methodologies has empowered the characterization complex biological samples by dramatically increasing number constituent cells that can be examined concurrently. Nevertheless, these approaches typically recover substantially less information per-cell as compared to lower-throughput microtiter plate-based strategies. To uncover critical phenotypic differences among and effectively link scRNA-Seq observations...
Cytosolic detection of nucleic acids elicits a type I interferon (IFN) response and plays critical role in host defense against intracellular pathogens. Herein, global gene expression profile Mycobacterium leprae–infected primary human Schwann cells identified the genes differentially expressed IFN pathway. Among them, encoding 2′-5′ oligoadenylate synthetase-like (OASL) underwent greatest upregulation was also shown to be upregulated M. macrophage cell lineages, monocytes, skin lesion...
Leprosy, the leading infectious cause of disability worldwide, remains a major public health challenge in most severely affected countries despite sharp decline new cases recent years. The search for biomarkers is essential to achieve better understanding molecular and cellular mechanisms underlying disease.Pentraxin-3 (PTX3) analyses sera from 87 leprosy patients with or without reactions were conducted via enzyme-linked immunosorbent assay. In situ identification PTX3 skin lesion was...
Leprosy reactions are responsible for incapacities in leprosy and represent the major cause of permanent neuropathy. The identification biomarkers able to identify patients more prone develop reaction could contribute adequate clinical management prevention incapacities. Reversal may occur unstable borderline also lepromatous patients. To biomarker signature profiles related with reversal onset, multibacillary were recruited classified accordingly occurrence or not during after...
Introduction The COVID-19 pandemic has highlighted the need to identify mechanisms of antiviral host defense against SARS-CoV-2. One such mediator is interferon-g (IFN-γ), which, when administered infected patients, reported result in viral clearance and resolution pulmonary symptoms. IFN-γ treatment a human lung epithelial cell line triggered an activity SARS-CoV-2, yet mechanism for this response was not identified. Methods Given that been shown trigger via generation nitric oxide (NO), we...
Abstract Granulomas are complex cellular structures comprised predominantly of macrophages and lymphocytes that function to contain kill invading pathogens. Here, we investigated single cell phenotypes associated with antimicrobial responses in human leprosy granulomas by applying spatial sequencing biopsy specimens. We focused on reversal reactions (RR), a dynamic process which some patients disseminated lepromatous (L-lep) transition towards self-limiting tuberculoid (T-lep), mounting...
Leprosy reactional episodes are acute inflammatory events that may occur during the clinical course of disease. Type 1 reaction (T1R) is associated with an increase in neural damage, and understanding molecular pathways related to T1R onset pivotal for development strategies effectively control reaction. Interferon-gamma (IFN-γ) a key cytokine also autophagy induction. Here, we evaluated modulation pathway Mycobacterium leprae-stimulated cells presence or absence IFN-γ. We observed IFN-γ...
Langerhans cells (LCs) reside in the epidermis where they are poised to mount an antimicrobial response against microbial pathogens invading from outside environment. To elucidate potential pathways by which LCs contribute host defense, we mined published LC transcriptomes deposited GEO and scientific literature for genes that participate responses. Overall, identified 31 encode proteins activity, ten of were cross-validated at least two separate experiments. Seven these chemokines, CCL1,...
Abstract The immune response to intracellular bacteria is critical for host defense against the invading pathogen. This interaction can be studied in human disease leprosy caused by bacterium Mycobacterium leprae affecting primarily skin compartments. Patients with tuberculoid (T-lep) express a Th1 cytokine pattern lesions and develop protective immunity that eliminates infection, whereas those lepromatous (L-lep) Th2 cytokines sustain progressive infection. In reversal reaction (RR) state,...