A5057063160- Extracellular vesicles in disease
- Tuberculosis Research and Epidemiology
- Glioma Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- HER2/EGFR in Cancer Research
- Gastric Cancer Management and Outcomes
- Nanoplatforms for cancer theranostics
- Phagocytosis and Immune Regulation
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Cancer, Lipids, and Metabolism
- Galectins and Cancer Biology
- Infectious Diseases and Tuberculosis
- Cancer Immunotherapy and Biomarkers
- Click Chemistry and Applications
- Mitochondrial Function and Pathology
- Cancer Mechanisms and Therapy
- Photochromic and Fluorescence Chemistry
- Axon Guidance and Neuronal Signaling
University Medical Center Hamburg-Eppendorf
2013-2024
Universität Hamburg
2013-2024
Michigan Medicine
2019-2022
Research Center Borstel - Leibniz Lung Center
2016-2022
Leipzig University
2021-2022
German Center for Infection Research
2022
University Medical Center
2022
Institut für Soziale Arbeit
2021
Freie Universität Berlin
2021
Deutsche Nationalbibliothek
2021
ABSTRACT Cells release heterogeneous nano‐sized vesicles either as exosomes, being derived from endosomal compartments, or through budding the plasma membrane so‐called microvesicles, commonly referred to extracellular (EVs). EVs are known for their important roles in mammalian physiology and disease pathogenesis provide a potential biomarker source cancer patients. generally often analysed bulk using Western blotting by bead‐based flow‐cytometry or, with limited parameters, nanoparticle...
Trastuzumab is the only approved targeted drug for first-line treatment of human epidermal growth factor receptor 2-positive (HER2+) metastatic gastric cancer (mGC). However, not all patients respond and most eventually progress. The multicenter VARIANZ study aimed to investigate background response resistance trastuzumab in mGC.Patients receiving medical mGC were prospectively recruited 35 German sites followed up 48 months. HER2 status was assessed centrally by immunohistochemistry...
Abstract Background Extracellular vesicles (EVs) obtained by noninvasive liquid biopsy from patient blood can serve as biomarkers. Here, we investigated the potential of circulating plasma EVs to an indicator in diagnosis, prognosis, and treatment response glioblastoma patients. Methods Plasma samples were collected patients at multiple timepoints before after surgery. EV concentrations measured nanoparticle tracking analysis imaging flow cytometry. Tumor burden edema quantified 3D...
Background.The treatment efficacy of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors like erlotinib has not met expectations for glioblastoma therapy, even EGFR-overexpressing tumors.We determined possible mechanisms therapy resistance using the unique BS153 cell line, which retained amplification egfr gene and expression EGFR variant (v)III.Methods.Functional effects erlotinib, gefitinib, cetuximab on proliferation, migration, EGFR-dependent signal transduction were...
Immunotherapeutic strategies are increasingly important in neuro-oncology, and the elucidation of escape mechanisms that lead to treatment resistance is crucial. We investigated impact immune pressure on clonal dynamics signature by comparing glioma growth immunocompetent versus immunodeficient mice. Glioma-bearing WT Pd-1-/- mice survived significantly longer than Pfp-/- Rag2-/- While tumors were highly polyclonal, immunoedited displayed reduced clonality with emergence clones. Tumor cells...
Extracellular vesicles (EVs) are known for their important role in cancer progression and hold considerable potential as a source tumor biomarkers. However, purification of tumor-specific EVs from patient plasma is still an urgent unmet need due to contamination by normal host cell-derived EVs, that results compromised analytical sensitivity. Here we identified fatty acid synthase (FASN), key lipogenic enzyme which highly expressed malignant glioma cells, be elevated CD63- CD81-positive...
Extracellular vesicles (EVs) play an important role in cell-cell communication, and tumor-derived EVs circulating patient blood can serve as biomarkers. Here, we investigated the potential of plasma meningioma patients for tumor detection determined whether secreted by cells reflect epigenetic, genomic, proteomic alterations original tumors.EV concentrations were quantified (n = 46). Short-term cultures established 26) isolated. Methylation copy number profiling was performed using 850k...
Abstract A series of precision glycomacromolecules is prepared following previously established solid phase synthesis allowing for controlled variations interligand spacing and the overall number carbohydrate ligands. In addition, now also different linkers are installed between ligand macromolecular scaffold. The lectin binding behavior these then evaluated in isothermal titration calorimetry (ITC) kinITC experiments using Concanavalin (Con A) its dimeric tetrameric form. results indicate...
In order to allow spatial and temporal control of carbohydrate-specific bacterial adhesion, it has become our goal synthesise azobenzene mannosides as photoswitchable inhibitors type 1 fimbriae-mediated adhesion E. coli. An mannobioside 2 was prepared its photochromic properties were investigated. The E→Z isomerisation found be highly effective, yielding a long-lived (Z)-isomer. Both isomers, E Z, show excellent water solubility tested mannoside-specific in solution. Their inhibitory potency...
Mycobacterium tuberculosis resides in the lungs various lesion types with unique microenvironmental conditions. This diversity is line heterogeneous disease progression and divergent drug efficiency. Fluorescent reporter strains can be used to decipher micromilieu guide future treatment regimens. Current reporters using replicating plasmids, however, are not suitable for long-term mouse infections or studies non-human primates. Using a combination of recombinant DNA protein optimization...
The CRISPR/Cas system has a broad range of possible medical applications, but its clinical translation been hampered, particularly by the lack safe and efficient vector systems mediating short-term expression components. Recently, different virus-like particles (VLPs) have introduced as promising vectors for delivery genome editing Here, we characterized directly compared three types retrovirus-based (R) VLPs, two derived from γ-retrovirus murine leukemia virus (gRVLPs "enhanced" egRVLPs)...
Abstract Mycobacterium tuberculosis (Mtb), the main causative agent of (Tb), has a complex cell envelope which forms an efficient barrier to antibiotics, thus contributing challenges anti‐tuberculosis therapy. However, unique Mtb wall can be considered advantage and utilized selectively label bacteria. Here we introduce three azido pentoses as new compounds for metabolic labeling Mtb: 3‐azido arabinose (3AraAz), ribose (3RiboAz), 5‐azido arabinofuranose (5AraAz). 5AraAz demonstrated highest...
Lalistat inhibits growth of<italic>Mycobacterium tuberculosis</italic>in bacterial culture as well in infected macrophages.
Glycoarrays—easier than ever: Glycoarrays were fabricated on polystyrene microtiter plates with great ease by using a tandem process that combined hydrophobic adsorption and thiourea bridging. They validated testing specific bacterial adhesion its inhibition.
BRI family proteins are ubiquitous type II transmembrane but BRI2 is highly expressed in some neuronal tissues. Possible functions include maturation and differentiation. Protein complexes appear to be important mediating its functions. Previously described interactors the Alzheimer's amyloid precursor protein phosphatase 1, clearly identification of novel provides an tool understand role function BRI2. To this end three rat brain regions (cerebellum, hippocampus, cerebral cortex) were...
Background: Hard-to-heal wounds are associated with high treatment costs and, in Germany, mostly treated the outpatient care sector. Wound dressings main cost-drivers venous leg ulcer (VLU) which prescription is budget-restricted.Objective: To determine to what extent choice of antimicrobial dressing affects spending by investigating budget impact bioburden-reducing Cutimed Sorbact.Methods: The analysis was performed comparing three different scenarios intervention mix dressings. A Markov...
Rhizochalinin (Rhiz) is a recently discovered cytotoxic sphingolipid synthesized from the marine natural compound rhizochalin. Previously, Rhiz demonstrated high in vitro and vivo efficacy various cancer models. Here, we report to be highly active human glioblastoma cell lines as well patient-derived glioma-stem like neurosphere counteracted proliferation by inducing apoptosis, G2/M-phase cycle arrest, inhibition of autophagy. Proteomic profiling followed bioinformatic analysis suggested...
BackgroundFixed-dose combinations of inhaled corticosteroids and long-acting β2 agonists are commonly used for the treatment asthma COPD. However, most frequently prescribed dry powder inhaler delivering this medicine – Symbicort® (budesonide formoterol, BF) Turbuhaler® is associated with poor inhalation technique, which can lead to disease control high management costs. A recent study showed that patients make fewer errors when using novel DuoResp® (BF) Spiromax® inhaler, compared BF...